Alignments for a candidate for dddA in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for dddA in Pseudomonas putida KT2440

Align 3-hydroxypropionate dehydrogenase (EC 1.1.1.59) (characterized)
to candidate PP_5064 PP_5064 choline dehydrogenase

Query= metacyc::MONOMER-15202
         (579 letters)



>FitnessBrowser__Putida:PP_5064
          Length = 565

 Score =  344 bits (883), Expect = 5e-99
 Identities = 220/546 (40%), Positives = 305/546 (55%), Gaps = 33/546 (6%)

Query: 36  FDYIVVGAGTAGCLLANRLSADPANRVLLIEAGGRDNYHW---IHIPVGYLYCINNPRTD 92
           FDYI+VGAG+AG  LA RL+ D    VLL+EAGG D Y +     +P    + +   R +
Sbjct: 5   FDYIIVGAGSAGNTLATRLTEDAGVTVLLLEAGGPD-YRFDFRTQMPAALAFPLQGRRYN 63

Query: 93  WRFRTEPDPGLNGRSLIYPRGKTLGGCSSINGMLYLRGQARDYDGWAELTGDDAWRWDNC 152
           W + T+P+P ++GR +   RGK LGG S INGM Y+RG A D+DGWAEL G + W + +C
Sbjct: 64  WAYETDPEPYMDGRRMECGRGKGLGGSSLINGMCYIRGNAMDFDGWAELPGLEDWTYLDC 123

Query: 153 LPDFMRHEDHYRLDEGGDADPDHYKFHGHGGEWRIEKQRLKW---QVLADFATAAVEAGV 209
           LP       ++R  E  D  P+ Y    HGGE  +     K     +      A V+AG 
Sbjct: 124 LP-------YFRKAETRDIGPNDY----HGGEGPVSVTTPKAGNNPLFHAMVEAGVQAGY 172

Query: 210 PRTRDFNRGDNEGVDAFEVN-QRSGWRWNASKAFLRGVEQRGNLTVWHSTQVLKLDFASG 268
           PRT D N    EG    +    ++G R + ++ +L   ++R NLT+       ++ F   
Sbjct: 173 PRTEDLNGYQQEGFGPMDRTVTKNGRRSSTARGYLDQAKKRPNLTIVTHALTDRVLFDG- 231

Query: 269 EGSEPRCCGVT--VERAGKKVVTTARCEVVLSAGAIGSPQLLQLSGIGPTALLAEHAIPV 326
                R  GVT  V  + ++V   AR EV++S+GAI SPQLLQ SG+GP ALL    IPV
Sbjct: 232 ----KRAIGVTYLVGDSEERVEARARKEVIVSSGAIASPQLLQRSGVGPRALLESLDIPV 287

Query: 327 VADLPGVGENLQDHLQIRSIYKVKGAKTLNTMANSLI--GKAKIGLEYILKRSGPMSMAP 384
           V DLPGVGENLQDHL++   Y      +L     SL+   +  IG E++   +G  +   
Sbjct: 288 VHDLPGVGENLQDHLELYLQYACTQPVSLYP---SLLWWNQPAIGAEWMFNGTGIGASNQ 344

Query: 385 SQLCIFTRSSKEYEHPNLEYHVQPLSLEAFGQPLHDFPAITASVCNLNPTSRGTVRIKSG 444
            +   F R+  E++ PN++YH  P+++   G          A + ++   +RG +++KS 
Sbjct: 345 FEAGGFIRTRPEFKWPNIQYHFLPVAINYNGSNGVKEHGFQAHMGSMRSPARGRIQVKSK 404

Query: 445 NPRQAPAISPNYLSTEEDRQVAADSLRVTRHIASQPAFAKYDPEEFKPGVQYQSDEDLAR 504
           +PRQ P+I  NY+STE+D Q   D +R+TR I +QPA   Y   E  PG   Q+DE L +
Sbjct: 405 DPRQHPSILFNYMSTEQDWQEFRDGIRLTREIMAQPALDPYRGREISPGADVQTDEQLDK 464

Query: 505 LAGDIGTTIFHPVGTAKMGRDDDPMAVVDSHLRVRGVTGLRVVDASIMPTITSGNTNSPT 564
              +   T FHP  + KMG DD  MAVVD   RV G+ GLRVVDASIMP I +GN N+ T
Sbjct: 465 FIREHAETAFHPSCSCKMGTDD--MAVVDGEGRVHGMKGLRVVDASIMPLIITGNLNATT 522

Query: 565 LMIAEK 570
           +MIAEK
Sbjct: 523 IMIAEK 528


Lambda     K      H
   0.318    0.135    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 988
Number of extensions: 52
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 565
Length adjustment: 36
Effective length of query: 543
Effective length of database: 529
Effective search space:   287247
Effective search space used:   287247
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources