GapMind for catabolism of small carbon sources

 

Aligments for a candidate for malK_Sm in Pseudomonas putida KT2440

Align MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized)
to candidate PP_5179 PP_5179 spermidine/putrescine ABC transporter - ATP binding subunit

Query= TCDB::Q8DT25
         (377 letters)



>lcl|FitnessBrowser__Putida:PP_5179 PP_5179 spermidine/putrescine
           ABC transporter - ATP binding subunit
          Length = 380

 Score =  211 bits (538), Expect = 2e-59
 Identities = 129/340 (37%), Positives = 197/340 (57%), Gaps = 26/340 (7%)

Query: 4   LKLDNIYKRYPNAKHYSVENFNLDIHDKEFIVFVGPSGCGKSTTLRMIAGLEDITEGNLY 63
           +K+D + K++      +V++ +L+I   E    +G SG GKST LRM+AG E  +EG ++
Sbjct: 23  VKIDRVTKKFDET--VAVDDVSLEIRKGEIFALLGGSGSGKSTLLRMLAGFERPSEGRIF 80

Query: 64  IDDKLMNDASPKDRDIAMVFQNYALYPHMSVYENMAFGLKLRKYKKDDINKRVHEAAEIL 123
           +D   + D  P +R I M+FQ+YAL+PHM+V +N+AFGL+  K  K +I+ RV E  +++
Sbjct: 81  LDGVDITDMPPYERPINMMFQSYALFPHMTVAQNIAFGLQQDKMPKAEIDARVAEMLKLV 140

Query: 124 GLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMDEPLSNLDAKLRVAMRAEIAKIHR 183
            +T++ +RKP  LSGGQRQRVA+ R++ +  K+ L+DEP+  LD KLR  M+ E+ +I  
Sbjct: 141 HMTQYAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRSQMQLELVEIIE 200

Query: 184 RIGATTIYVTHDQTEAMTLADRIVIMSATPNPDKTGSIGRIEQIGTPQELYNEPANKFVA 243
           R+G T + VTHDQ EAMT+A RI IM           +G I QIG+P ++Y  P ++ V 
Sbjct: 201 RVGVTCVMVTHDQEEAMTMAQRIAIM----------HLGWIAQIGSPVDIYETPTSRLVC 250

Query: 244 GFIGSPAMNFFEVTVEKERLVNQDGLSLALPQGQEKILEEKGYL----GKKVTLGIRPED 299
            FIG+  +N FE  V  +    +    +A P+ + KI    G       K +T  +RPE 
Sbjct: 251 EFIGN--VNLFEGDVVDDA---EGYAIIASPELERKIYVGHGITTSVEDKHITYALRPEK 305

Query: 300 --ISSDQIVHETFPNASVTADILVSELLGSESMLYVKFGS 337
             +++ Q   E   +     DI     LG  S+ YV+  S
Sbjct: 306 MLVTTQQPTCEHNWSRGKIHDI---AYLGGHSVFYVELPS 342


Lambda     K      H
   0.318    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 330
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 377
Length of database: 380
Length adjustment: 30
Effective length of query: 347
Effective length of database: 350
Effective search space:   121450
Effective search space used:   121450
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory