Align ABC-type maltose transporter (EC 7.5.2.1) (characterized)
to candidate PP_1722 PP_1722 ABC transporter, ATP-binding protein
Query= BRENDA::Q8NMV1 (376 letters) >FitnessBrowser__Putida:PP_1722 Length = 329 Score = 218 bits (555), Expect = 2e-61 Identities = 122/293 (41%), Positives = 179/293 (61%), Gaps = 18/293 (6%) Query: 1 MATVTFKDASLSYPGAKEPTVKKFNLEIADGEFLVLVGPSGCGKSTTLRMLAGLENVTDG 60 M+ V+ + SY G+ P ++ + I GEF+ L+GPSGCGKST LR +AGL +V G Sbjct: 1 MSFVSVQKLQKSYAGS--PVFERIDCHIERGEFVTLLGPSGCGKSTLLRCIAGLTSVDSG 58 Query: 61 AIFIGDKDVTHVAPRDRDIAMVFQNYALYPHMTVGENMGFALKIAGKSQDEINKRVDEAA 120 I + D+ ++P+ R I MVFQ+YAL+P+MTV +N+ F L++ DE RV E Sbjct: 59 QILLDGHDIVPLSPQKRGIGMVFQSYALFPNMTVEQNVAFGLRMQKVKADESQLRVREVL 118 Query: 121 ATLGLTEFLERKPKALSGGQRQRVAMGRAIVRNPQVFLMDEPLSNLDAKLRVQTRTQIAA 180 + L +F R P LSGGQ QRVA+ R++V P++ L+DEPLS LDA++R R QI A Sbjct: 119 ELVELGKFAGRYPHQLSGGQCQRVALARSLVTRPRLLLLDEPLSALDARIRKHLREQIRA 178 Query: 181 LQRKLGVTTVYVTHDQTEALTMGDRIAVLKDGYLQQVGAPRELYDRPANVFVAGFIGSPA 240 +QR+LG+TT++VTHDQ EALTM DRI ++ G + Q G LY P ++F AGFIG+ Sbjct: 179 IQRELGLTTIFVTHDQEEALTMSDRIFLMNQGRIVQSGDAETLYTAPVDLFAAGFIGN-- 236 Query: 241 MNLGTFSVKDGDATSG------HARIKLSPETLAAMTPEDNGRITIGFRPEAL 287 +++ D D+ S +R+ + PE++ T ++G + R +L Sbjct: 237 -----YNLLDADSASRLLQRPVASRLAIRPESI---TLGEHGELDAEVRSHSL 281 Lambda K H 0.316 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 289 Number of extensions: 13 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 329 Length adjustment: 29 Effective length of query: 347 Effective length of database: 300 Effective search space: 104100 Effective search space used: 104100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory