Align mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17); mannitol-1-phosphatase (EC 3.1.3.22) (characterized)
to candidate PP_2052 PP_2052 putative bifunctional enzyme: sugar-phosphatase/mannitol-1-phosphate 5-dehydrogenase
Query= BRENDA::Q6FBP5 (713 letters) >FitnessBrowser__Putida:PP_2052 Length = 707 Score = 632 bits (1629), Expect = 0.0 Identities = 329/648 (50%), Positives = 440/648 (67%), Gaps = 12/648 (1%) Query: 1 MLIFHNYSVQGALFDMDGTMFDTERLRFQTLKQASQELLGQTFSDDYLMQCLGLSATTAE 60 ML FH + A+FDMDGT+FDTERLRF+TLKQAS E+ G+ S+ L+ LGLSA AE Sbjct: 1 MLFFHGKQIFSAIFDMDGTLFDTERLRFRTLKQASLEIFGKALSEQTLIGSLGLSAKKAE 60 Query: 61 QLAKSQYGDDVPYKAIRKCADDLELEWVRRDGVPIKKGLVQVLERLRKSGLRMAVATSSR 120 LAK+ G+D PY IR+ AD+LELE+VR GVPIK GL++VLERLRKSGL MAVATSSR Sbjct: 61 ALAKAHNGEDFPYAQIRQRADELELEYVRNHGVPIKAGLLEVLERLRKSGLTMAVATSSR 120 Query: 121 RAIAEEYLINANVYKFFDVLVCGDEVQQGKPHPEIFEKAAQKLNLDPAQCLMFEDSENGI 180 RAIAEEYLINANV K+FD+ VCGDEV QGKPHPEIF KAA+ LN +P QC M EDSENG+ Sbjct: 121 RAIAEEYLINANVLKYFDITVCGDEVGQGKPHPEIFLKAARALNCEPGQCFMVEDSENGM 180 Query: 181 SSAHAAGGITILLKDIKSPNEHMLSKANFYFETMYDCLLNLDDYVPIMDMPLLQETFPQT 240 SA A G IL++DIK P + + A + +M++ L +L + VP + MP L E FP + Sbjct: 181 LSAMRAEGQAILIEDIKPPAPEIKAGALKAYRSMHEFLADLSECVPDLGMPALSEPFPAS 240 Query: 241 LNQLTVGIHGFGAIGGGYIAQILSHWDGYTRPQRIIASTRNSLYREAVNAFGTYSIRYGQ 300 +NQ +VGIHGFGAIGGGY+ Q+ SHWDGYTRP+ IIA+TR+ + RE+V+AFG YS+RYG Sbjct: 241 MNQFSVGIHGFGAIGGGYLTQVFSHWDGYTRPREIIAATRSRMLRESVSAFGRYSVRYGA 300 Query: 301 VSYDERIEYMSIIDADNEQHMLDMYLESSLIALCIPEHAVASEAKIIAKGLYARYASQTM 360 S+D+ I+ + +ID D++ ++ MY + +I L +PE A+ S+A++IA+GL R+ Sbjct: 301 TSFDQTIDNVRMIDMDDDDAVISMYTTAEIIGLSLPEQAIRSQARVIAQGLLQRFE---- 356 Query: 361 QNNNPLTFLIILNKVGAKKMVLEHIRKALENLANLEVADQIMAQHYFCDTVVNRMVSKLS 420 + LT LI+LNKVG V H++ L L +++Q++ + +F +TVV+R+VSKL Sbjct: 357 RRGRELTLLIVLNKVGGAAFVRRHVQAELSLLCPPAISEQVLQKTHFAETVVSRIVSKLG 416 Query: 421 EQNLYRQLQIKYNFFKQYQSDVDDENVEIEDTAKLSTAQEHQASLYIDDMRRNFQPSHIL 480 L RQL+IK F Q+ ++DE T K ST L I R QPS + Sbjct: 417 NDALVRQLRIKSQMF---QNSLEDEPA---STRKSSTPLPEYERL-IGHFRPFAQPSSAM 469 Query: 481 QSMDLILFNSEIDMPIYVENNSPLLEKMRQMIVVDNIADIQLIKNRLWNGTHAMLAWYAS 540 + L+LFNSE DMP+Y E S LLE++RQ+ V +IA IQ+IKNRLWNG HA++AWYAS Sbjct: 470 SQLHLVLFNSEADMPLYAERGSDLLERLRQVKTVPDIAQIQIIKNRLWNGPHAIVAWYAS 529 Query: 541 LLGHETIGVAMGDIRIQKMVNNLV-DEIKAGLATQLPHRAKDLDRLAKSFIVSCQNAYKD 599 LLGH +G MGD R+ ++ L+ E+ L + P A+ + R A++F+ C+ ++KD Sbjct: 530 LLGHAWVGQGMGDARVSELAERLIRQEVAPALVAEYPQMAEVVSRFAEAFLERCKTSFKD 589 Query: 600 PCQRVARDPLRKLNPNERVLGSIVNNLRYHQTVDQLLWGASLGYFYAI 647 PC RV RDPLRKL NER+L SI ++ L +GA L +A+ Sbjct: 590 PCARVGRDPLRKLQRNERILSSIDLARKHGIATPALAFGAGLAIHHAL 637 Lambda K H 0.321 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1062 Number of extensions: 37 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 713 Length of database: 707 Length adjustment: 39 Effective length of query: 674 Effective length of database: 668 Effective search space: 450232 Effective search space used: 450232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory