Alignments for a candidate for PS417_11890 in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_11890 in Pseudomonas putida KT2440

Align Inositol transport system ATP-binding protein (characterized)
to candidate PP_2455 PP_2455 ribose ABC transporter - ATP-binding subunit

Query= reanno::WCS417:GFF2332
         (517 letters)



>FitnessBrowser__Putida:PP_2455
          Length = 524

 Score =  336 bits (862), Expect = 1e-96
 Identities = 185/475 (38%), Positives = 294/475 (61%), Gaps = 4/475 (0%)

Query: 39  LADVQLRVRPGTVLALMGENGAGKSTLMKIIAGIYQPDAGEIRLRGKPIVFETPLAAQKA 98
           L +V L +R G VLAL GENGAGKSTL K+I+G+  P  G +  RG+     +   A++ 
Sbjct: 31  LGEVSLSLRAGEVLALTGENGAGKSTLSKLISGLEVPTTGHMTYRGQAYAPGSRGEAERL 90

Query: 99  GIAMIHQELNLMPHMSIAENIWIGREQLNSLHMVNHREMHRCTAELLARLRIN-LDPEEQ 157
           G+ M+ QELNL+P +++AEN+++     +    ++H+ + +     +AR+ ++ +DP+  
Sbjct: 91  GVRMVMQELNLLPTLTVAENLFLDNLP-SRFGWISHKRLRQLATAAMARVGLDAIDPDTP 149

Query: 158 VGNLSIAERQMVEIAKAVSYDSDILIMDEPTSAITEKEVAHLFSIIADLKSQGKGIVYIT 217
           VG L I  +QMVEIA+ +  D  +LI+DEPT+ +T +EVA LF+ I  L+++G  IVYI+
Sbjct: 150 VGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVALLFTQIERLRARGVAIVYIS 209

Query: 218 HKMNEVFAIADEVAVFRDGHYIGLQRADSMNSDSLISMMVGRELSQLFPLRETPIGDLLL 277
           H++ E+  +A  + V RDG  +  +     +S  L+++MVGREL +   L    +G  LL
Sbjct: 210 HRLEELQRVAQRIVVLRDGKLVCDEPIQRYSSAELVNLMVGRELGEHIDLGRRQLGAPLL 269

Query: 278 TVRDLTLDGVFKDVSFDLHAGEILGIAGLMGSGRTNVAETIFGITPSSSGQITL--DGKA 335
            V  L      ++VSF++ AGEI GI+GL+G+GRT +   I+G   + SG I L    +A
Sbjct: 270 KVDKLCRGDKVREVSFEVRAGEIFGISGLIGAGRTELLRLIYGADRADSGGIALGQPPQA 329

Query: 336 VRISDPHMAIEKGFALLTEDRKLSGLFPCLSVLENMEMAVLPHYTGNGFIQQKALRALCE 395
           V I  P  A+  G AL+TEDRK  GL    S+  N+ +  L   +  G +  +A +AL E
Sbjct: 330 VSIDSPKAAVRAGIALITEDRKGEGLLLTQSISANIALGNLGAVSRAGVLDSEAEKALAE 389

Query: 396 DMCKKLRVKTPSLEQCIDTLSGGNQQKALLARWLMTNPRLLILDEPTRGIDVGAKAEIYR 455
              + +R+++   +Q +  LSGGNQQK ++ RWL  + ++L+ DEPTRGIDVGAK +IY 
Sbjct: 390 RQIQAMRIRSAGAQQVVGELSGGNQQKVVIGRWLERDCQVLLFDEPTRGIDVGAKFDIYG 449

Query: 456 LIAFLASEGMAVIMISSELPEVLGMSDRVMVMHEGELMGTLDRSEATQEKVMQLA 510
           L+A LA +G A++++SS+L E++ + DR+ V+  G L+ T  R   +Q++++  A
Sbjct: 450 LLAELARQGKALVVVSSDLRELMLICDRIAVLSAGRLIDTFARDHWSQDQLLAAA 504



 Score = 88.6 bits (218), Expect = 5e-22
 Identities = 60/207 (28%), Positives = 109/207 (52%), Gaps = 5/207 (2%)

Query: 287 VFKDVSFDLHAGEILGIAGLMGSGRTNVAETIFGITPSSSGQITLDGKAVRISDPHMAIE 346
           V  +VS  L AGE+L + G  G+G++ +++ I G+   ++G +T  G+A        A  
Sbjct: 30  VLGEVSLSLRAGEVLALTGENGAGKSTLSKLISGLEVPTTGHMTYRGQAYAPGSRGEAER 89

Query: 347 KGFALLTEDRKLSGLFPCLSVLENMEMAVLPHYTGNGFIQQKALRALCEDMCKKLRVKTP 406
            G  ++ ++     L P L+V EN+ +  LP  +  G+I  K LR L      ++ +   
Sbjct: 90  LGVRMVMQE---LNLLPTLTVAENLFLDNLP--SRFGWISHKRLRQLATAAMARVGLDAI 144

Query: 407 SLEQCIDTLSGGNQQKALLARWLMTNPRLLILDEPTRGIDVGAKAEIYRLIAFLASEGMA 466
             +  +  L  G+QQ   +AR L+ +  +LILDEPT  +     A ++  I  L + G+A
Sbjct: 145 DPDTPVGELGIGHQQMVEIARNLIGDCHVLILDEPTAMLTAREVALLFTQIERLRARGVA 204

Query: 467 VIMISSELPEVLGMSDRVMVMHEGELM 493
           ++ IS  L E+  ++ R++V+ +G+L+
Sbjct: 205 IVYISHRLEELQRVAQRIVVLRDGKLV 231


Lambda     K      H
   0.320    0.136    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 611
Number of extensions: 28
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 517
Length of database: 524
Length adjustment: 35
Effective length of query: 482
Effective length of database: 489
Effective search space:   235698
Effective search space used:   235698
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources