Alignments for a candidate for aroP in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for aroP in Pseudomonas putida KT2440

Align Aromatic amino acid transport protein AroP (characterized, see rationale)
to candidate PP_0927 PP_0927 aromatic amino acid transport protein

Query= uniprot:A0A0C4YP23
         (465 letters)



>FitnessBrowser__Putida:PP_0927
          Length = 453

 Score =  675 bits (1741), Expect = 0.0
 Identities = 329/446 (73%), Positives = 388/446 (86%)

Query: 8   NTDATLKRGLKNRHIQLIALGGAIGTGLFLGIAQTIKMAGPSVLLGYAVAGIIAFFIMRQ 67
           +T   L+RGLKNRHIQLIALGGAIGTGLFLGIAQTI++AGPSVLLGYA+AG++AF IMRQ
Sbjct: 5   STPERLQRGLKNRHIQLIALGGAIGTGLFLGIAQTIQLAGPSVLLGYAIAGLMAFLIMRQ 64

Query: 68  LGEMVVDEPVAGSFSHFANKYCGSFAGFMSGWNYWVLYILVSMAELSAVGIYVQYWWPHI 127
           LGEMVV+EPVAGSFSHFA++Y   FAGF+SGWNYWV+Y+LV MAEL+AVGIYVQYWWP  
Sbjct: 65  LGEMVVEEPVAGSFSHFAHQYWSEFAGFVSGWNYWVVYVLVGMAELTAVGIYVQYWWPDF 124

Query: 128 PTWASALGFFLLINAINLTSVKSFGEMEFWFSIVKVLAIVGMIVFGGYLLASGTAGPQAS 187
           PTWA+A  FF++IN INLT VK +GEMEFWF++VKV+AIV MI FG +LL SG  GP AS
Sbjct: 125 PTWATAAIFFVVINLINLTQVKVYGEMEFWFALVKVVAIVSMIGFGAWLLGSGHGGPDAS 184

Query: 188 VSNLWQHGGFFPNGISGLVMAMAVIMFSFGGLELVGITAAEADEPEKTIPKATNQVIYRI 247
           V+NLWQ+GGFFPNG++GLVMA+AVIMFSFGGLELVGITAAEAD P ++IPKATNQV+YRI
Sbjct: 185 VANLWQYGGFFPNGVTGLVMALAVIMFSFGGLELVGITAAEADNPRESIPKATNQVVYRI 244

Query: 248 LIFYVGALGVLLSLYPWEKVVTGGSPFVLIFHAMNSDIVATVLNAVVLTAALSVYNSGVY 307
           LIFY+GAL VLLSLYPW+KVV GGSPFV+IFH ++SD+VAT+LN VVLTAALSVYNS VY
Sbjct: 245 LIFYIGALAVLLSLYPWQKVVQGGSPFVMIFHELDSDLVATILNIVVLTAALSVYNSCVY 304

Query: 308 CNSRMLFGLAKQGNAPKALLKVNKRGIPLAALGVSALATAACVVINYFMPGEAFELLMGL 367
            NSRMLFGLA QG+AP+ LLKV++ G+PL AL VSA AT  CV+INY MPG AF LLM L
Sbjct: 305 ANSRMLFGLASQGDAPRQLLKVSRSGVPLTALAVSAFATGLCVLINYLMPGGAFGLLMAL 364

Query: 368 VVSALIINWAMISIIHLKFRRDKRAAGQETRFKSLGYPLTNYVCLAFLAGILYVMYLTPG 427
            VSAL+INWA ISI HLKFR+ K AAG    +KSLG+PLTNY+CLAF+  IL VMYLTP 
Sbjct: 365 AVSALVINWASISITHLKFRKAKLAAGITPFYKSLGHPLTNYLCLAFIVLILVVMYLTPP 424

Query: 428 LRISVYLIPAWLAVLGLSYRLRQKQK 453
           +RISV LIPAW+AVL ++++L++ ++
Sbjct: 425 IRISVMLIPAWIAVLWVAFKLKKARQ 450


Lambda     K      H
   0.326    0.140    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 688
Number of extensions: 27
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 465
Length of database: 453
Length adjustment: 33
Effective length of query: 432
Effective length of database: 420
Effective search space:   181440
Effective search space used:   181440
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources