Alignments for a candidate for pad-dh in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for pad-dh in Pseudomonas putida KT2440

Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate PP_2487 PP_2487 putative aldehyde dehydrogenase

Query= BRENDA::P05091
         (517 letters)



>FitnessBrowser__Putida:PP_2487
          Length = 503

 Score =  400 bits (1028), Expect = e-116
 Identities = 213/489 (43%), Positives = 305/489 (62%), Gaps = 10/489 (2%)

Query: 28  PNQQPEVFCNQIFINNEWHDAVSRKTFPTVNPSTGEVICQVAEGDKEDVDKAVKAARAAF 87
           PN  P+  C  +FI+N+W  A   +T   +NP+ G+++  +      DVD+AV+AA+ AF
Sbjct: 13  PNHLPDS-CYGLFIDNQWVTAEYGETLDIINPANGKILTNIPNATAADVDRAVQAAQRAF 71

Query: 88  QLGSPWRRMDASHRGRLLNRLADLIERDRTYLAALETLDNGKPYVISYLVDLDMVLKCLR 147
                WR    + R   L ++ADL+E D    A LETLD GKP   S  VD+ + +   R
Sbjct: 72  VT---WRTTSPAERANALLKIADLLEADADRFAVLETLDVGKPIRESRSVDIPLAIDHFR 128

Query: 148 YYAGWADKYHGKTIPIDGDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVV 207
           Y+AG       + + +D    S    EP+GV GQ+IPWNFPLLM AWK+ PA+A GN VV
Sbjct: 129 YFAGVIRSQSDEAVMLDEQTLSIALSEPLGVVGQVIPWNFPLLMAAWKIAPAIAAGNTVV 188

Query: 208 MKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIG 267
           +K +E TP+T L +A +  +   P GVVNIV G G T G A+  H D+ K+AFTGST +G
Sbjct: 189 IKPSELTPVTILELAKIFAKV-LPAGVVNIVTGLGTTVGQALLDHPDLRKLAFTGSTRVG 247

Query: 268 RVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFV 327
            ++  AA +  +   TLELGGKS NI+  DA+ D AVE A  A+ +NQGQ C +G+R FV
Sbjct: 248 ELVANAA-AKKIIPATLELGGKSANIVFPDANWDKAVEGAVLAILWNQGQVCESGARLFV 306

Query: 328 QEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCG 387
            E IY+ F+     + ++  VG+P +  T  G QV +TQ ++ILGY++  K+EGA++L G
Sbjct: 307 HESIYERFLAELKHKFEAVRVGDPLNPDTMMGAQVSKTQMERILGYVDIAKEEGAEVLIG 366

Query: 388 GGIAA----DRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYG 443
           GG       D G+FIQPT+   V++ M +A EEIFGPV+ ++ FK   EV+  AN+S YG
Sbjct: 367 GGRLTGADYDAGFFIQPTILVGVRNDMRVAYEEIFGPVLCVIPFKDEAEVIAMANDSEYG 426

Query: 444 LAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYT 503
           LA AV+T+D+++A  +++A++ G +WVN Y    A +PFGGYK SG GRE  +  L+AY+
Sbjct: 427 LAGAVWTQDINRALRVARAVETGRMWVNTYHEIPAHAPFGGYKKSGLGRETHKSMLEAYS 486

Query: 504 EVKTVTVKV 512
           + K + V +
Sbjct: 487 QKKNIYVSL 495


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 591
Number of extensions: 26
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 503
Length adjustment: 34
Effective length of query: 483
Effective length of database: 469
Effective search space:   226527
Effective search space used:   226527
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources