Align phenylacetaldehyde dehydrogenase monomer (EC 1.2.1.39) (characterized)
to candidate PP_3463 PP_3463 phenylacetaldehyde dehydrogenase
Query= metacyc::MONOMER-15732 (497 letters) >FitnessBrowser__Putida:PP_3463 Length = 497 Score = 981 bits (2535), Expect = 0.0 Identities = 486/497 (97%), Positives = 495/497 (99%) Query: 1 MLCELPILPATRAFLERKLKMRIGADWQDAASGRTLSFRNPATGEVLGEVPAADAEDVDR 60 ML ELPILPATRAFLERKLKMRIGADWQDAASGRTLSFRNPATGEVLGEVPAA+AEDVDR Sbjct: 1 MLSELPILPATRAFLERKLKMRIGADWQDAASGRTLSFRNPATGEVLGEVPAAEAEDVDR 60 Query: 61 AVRAARQAFDDSPWSRLRPRERQNLLWRLADLMERDARQLAELECLNNGKSAAVAQVMDV 120 AVRAARQAFDDSPWSR+RPRERQNLLWRLADLMERDARQLAELECLNNGKSAAVAQ+MDV Sbjct: 61 AVRAARQAFDDSPWSRMRPRERQNLLWRLADLMERDARQLAELECLNNGKSAAVAQMMDV 120 Query: 121 QLAIDFLRYMAGWATKIEGSTVEASMPLMPNDQFHGFVRREAIGVVGAIVAWNFPLLLAC 180 QLAIDFLRYMAGWATKIEGSTVEASMPLMPNDQFHGFVRREA+GVVGAIVAWNFPLLLAC Sbjct: 121 QLAIDFLRYMAGWATKIEGSTVEASMPLMPNDQFHGFVRREAVGVVGAIVAWNFPLLLAC 180 Query: 181 WKLGPALATGCTIVLKPADETPLSVLKLAELVDEAGYPAGVFNVVTGTGLNAGAALSRHP 240 WKLGPALATGCT+VLKPADETPLSVLKLAELVDEAGYPAGVFNVVTGTGLNAGAALSRHP Sbjct: 181 WKLGPALATGCTVVLKPADETPLSVLKLAELVDEAGYPAGVFNVVTGTGLNAGAALSRHP 240 Query: 241 GVDKLTFTGSTEVGKLIGKAAMDNMTRVTLELGGKSPTIVMPDANLQEAAAGAATAIFFN 300 GVDKLTFTGSTEVGKLIGKAAMDNMTRVTLELGGKSPTIVMPDANLQEAAAGAATAIFFN Sbjct: 241 GVDKLTFTGSTEVGKLIGKAAMDNMTRVTLELGGKSPTIVMPDANLQEAAAGAATAIFFN 300 Query: 301 QGQVCCAGSRLYVHRKHFDNVVADIAGIANGMKLGNGLDPAVQMGPLISAKQQDRVTGYI 360 QGQVCCAGSRLYVHRKHFDNVVADIAGIAN MKLGNGLDPAVQMGPLISAKQQDRVTGYI Sbjct: 301 QGQVCCAGSRLYVHRKHFDNVVADIAGIANAMKLGNGLDPAVQMGPLISAKQQDRVTGYI 360 Query: 361 ELGRELGATVACGGEGFGPGYFVKPTVIVDVDQRHRLVQEEIFGPVLVAMPFDDLDEVIG 420 ELGRELGAT+ACGGEGFGPGYFVKPTVIVDVDQRHRLVQEEIFGPVLVAMPFDD+DEVIG Sbjct: 361 ELGRELGATIACGGEGFGPGYFVKPTVIVDVDQRHRLVQEEIFGPVLVAMPFDDIDEVIG 420 Query: 421 MANDNPYGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGREVG 480 MANDNPYGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGRE+G Sbjct: 421 MANDNPYGLGASIWSNDLAAVHRMIPRIKSGSVWVNCHSALDPALPFGGYKMSGVGREMG 480 Query: 481 AAAIEHYTELKSVLIKL 497 +AAIEHYTELKSVLIKL Sbjct: 481 SAAIEHYTELKSVLIKL 497 Lambda K H 0.320 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1036 Number of extensions: 29 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 497 Length of database: 497 Length adjustment: 34 Effective length of query: 463 Effective length of database: 463 Effective search space: 214369 Effective search space used: 214369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory