Alignments for a candidate for actP in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Pseudomonas putida KT2440

Align Acetate permease; SubName: Full=Actetate permease; SubName: Full=Cation acetate symporter; SubName: Full=Cation/acetate symporter (characterized, see rationale)
to candidate PP_3272 PP_3272 acetate permease

Query= uniprot:A0A1N7U9G2
         (552 letters)



>FitnessBrowser__Putida:PP_3272
          Length = 520

 Score =  724 bits (1870), Expect = 0.0
 Identities = 354/520 (68%), Positives = 434/520 (83%)

Query: 33  LNVSAIVMFVVFVGATLCITYWASKRNKSAADYYAAGGKITGFQNGLAIAGDYMSAASFL 92
           +N +AI MF+VFV  TL +T WA+ R +SA+D+Y AGG +TG QNGLAIAGD +SAASFL
Sbjct: 1   MNWTAISMFMVFVCFTLLVTRWAALRTRSASDFYTAGGGLTGMQNGLAIAGDMISAASFL 60

Query: 93  GISALVYTSGYDGLIYSIGFLVGWPIILFLIAERLRNLGKYTFADVASYRLGQTQIRSLS 152
           GISA+++ +GYDGL+Y++G L GWPIILFLIAERLRNLGKYTFADV SYRL QT +R  S
Sbjct: 61  GISAMMFMNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLAQTPVRLTS 120

Query: 153 ACGSLVVVAFYLIAQMVGAGKLIQLLFGLDYHVAVILVGILMVLYVLFGGMLATTWVQII 212
           A G+LVV   YL+AQMVGAGKLI+LLFG+ Y  AV+LVG+LMV YV FGGMLATTWVQII
Sbjct: 121 AFGTLVVALMYLVAQMVGAGKLIELLFGISYLYAVMLVGVLMVSYVTFGGMLATTWVQII 180

Query: 213 KAVLLLSGASFMALMVMKHVNFDFNALFSEAIKVHPKGEAIMSPGGLVKDPISAFSLGLA 272
           KAV+LLSG SFMA MV+KH  F   A+F+ A+ VH KG+AIM+PGGL+ +P+ A SLGL 
Sbjct: 181 KAVMLLSGTSFMAFMVLKHFGFSTEAMFASAVAVHAKGQAIMAPGGLLSNPVDAISLGLG 240

Query: 273 LMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYILTFIIGFGAILLVSTNPAFK 332
           +MFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFY+L  ++GFGAI++V T P+++
Sbjct: 241 MMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLLIVVGFGAIVMVGTEPSYR 300

Query: 333 DAAGALMGGNNMAAVHLANAVGGSIFLGFISAVAFATILAVVAGLTLAGASAVSHDLYAS 392
           DA GA++GG NM AVHLA AVGG++FLGFISAVAFATILAVVAGL L+GASAVSHDLYA 
Sbjct: 301 DATGAIIGGGNMVAVHLAQAVGGNLFLGFISAVAFATILAVVAGLALSGASAVSHDLYAC 360

Query: 393 VIKKGKANDKDEIRVSKITTIALGVLAIGLGILFESQNIAFMVGLAFSIAASCNFPVLLL 452
           V++KGKA +++E+RVS+I T+ +G+LA+ LG++FESQNIAF+ GL  ++AAS NFPVLLL
Sbjct: 361 VMRKGKATEQEEMRVSRIATLLIGLLAVILGLMFESQNIAFLSGLVLAVAASVNFPVLLL 420

Query: 453 SMYWKNLTTRGAMIGGWLGLVSAVGLMVLGPTIWVSILHHEKAIFPYEYPALFSMIIAFV 512
           SM+WK LTTRGA+ G   GLVSAV L+VLGP +WV++LH+E A+FPY  PALFSM +AF+
Sbjct: 421 SMFWKGLTTRGAVCGSMAGLVSAVLLVVLGPAVWVNVLHNETALFPYSNPALFSMSLAFL 480

Query: 513 GIWFFSITDKSSAAEKERALYFPQFVRSQTGLGSSGAVSH 552
             W FS+TD S  A +ER  Y  QF+RS TG+G++GA  H
Sbjct: 481 SAWVFSVTDTSERAVEERGRYLGQFIRSMTGIGAAGASKH 520


Lambda     K      H
   0.327    0.140    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 953
Number of extensions: 34
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 552
Length of database: 520
Length adjustment: 35
Effective length of query: 517
Effective length of database: 485
Effective search space:   250745
Effective search space used:   250745
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources