Aligments for a candidate for mctC in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Aligments for a candidate for mctC in Pseudomonas putida KT2440

Align Monocarboxylic acid transporter (characterized)
to candidate PP_3272 PP_3272 acetate permease

Query= SwissProt::Q8NS49
         (551 letters)



>lcl|FitnessBrowser__Putida:PP_3272 PP_3272 acetate permease
          Length = 520

 Score =  434 bits (1117), Expect = e-126
 Identities = 242/537 (45%), Positives = 334/537 (62%), Gaps = 30/537 (5%)

Query: 23  ISVFVVFIIVTMTVVLRVGKSTSESTDFYTGGASFSGTQNGLAIAGDYLSAASFLGIVGA 82
           IS+F+VF+  T+ V       T  ++DFYT G   +G QNGLAIAGD +SAASFLGI   
Sbjct: 6   ISMFMVFVCFTLLVTRWAALRTRSASDFYTAGGGLTGMQNGLAIAGDMISAASFLGISAM 65

Query: 83  ISLNGYDGFLYSIGFFVAWLVALLLVAEPLRNVGRFTMADVLSFRLRQKPVRVAAACGTL 142
           + +NGYDG LY++G    W + L L+AE LRN+G++T ADV+S+RL Q PVR+ +A GTL
Sbjct: 66  MFMNGYDGLLYALGVLAGWPIILFLIAERLRNLGKYTFADVVSYRLAQTPVRLTSAFGTL 125

Query: 143 AVTLFYLIAQMAGAGSLVSVLLDIHEFKWQAVVVGIVGIVMIAYVLLGGMKGTTYVQMIK 202
            V L YL+AQM GAG L+ +L  I         V +VG++M++YV  GGM  TT+VQ+IK
Sbjct: 126 VVALMYLVAQMVGAGKLIELLFGIS----YLYAVMLVGVLMVSYVTFGGMLATTWVQIIK 181

Query: 203 AVLLVGGVAIMTVLTFVKVSGGLTTLLNDAVEKHAASDYAATKGYDPTQILEPGLQYGAT 262
           AV+L+ G + M  +           +   AV  HA       KG     I+ P    G  
Sbjct: 182 AVMLLSGTSFMAFMVLKHFGFSTEAMFASAVAVHA-------KG---QAIMAP----GGL 227

Query: 263 LTTQLDFISLALALCLGTAGLPHVLMRFYTVPTAKEARKSVTWAIVLIGAFYLMTLVLGY 322
           L+  +D ISL L +  GTAGLPH+LMRF+TV  AKEARKSV +A   IG FYL+ +V+G+
Sbjct: 228 LSNPVDAISLGLGMMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYLLLIVVGF 287

Query: 323 GAAALVGP-----DRVIAAPGAANAAAPLLAFELGGSIFMALISAVAFATVLAVVAGLAI 377
           GA  +VG      D   A  G  N  A  LA  +GG++F+  ISAVAFAT+LAVVAGLA+
Sbjct: 288 GAIVMVGTEPSYRDATGAIIGGGNMVAVHLAQAVGGNLFLGFISAVAFATILAVVAGLAL 347

Query: 378 TASAAVGHDIYNAVIRNGQSTEAEQVRVSRITVVVIGLISIVLGILAMTQNVAFLVALAF 437
           + ++AV HD+Y  V+R G++TE E++RVSRI  ++IGL++++LG++  +QN+AFL  L  
Sbjct: 348 SGASAVSHDLYACVMRKGKATEQEEMRVSRIATLLIGLLAVILGLMFESQNIAFLSGLVL 407

Query: 438 AVAASANLPTILYSLYWKKFNTTGAVAAIYTGLISALLLIFLSPAVSGNDSAMVPGADWA 497
           AVAAS N P +L S++WK   T GAV     GL+SA+LL+ L PAV  N    V   + A
Sbjct: 408 AVAASVNFPVLLLSMFWKGLTTRGAVCGSMAGLVSAVLLVVLGPAVWVN----VLHNETA 463

Query: 498 IFPLKNPGLVSIPLAFIAGWIGTLVGKPDNMDDLAAE---MEVRSLTGVGVEKAVDH 551
           +FP  NP L S+ LAF++ W+ ++    +   +         +RS+TG+G   A  H
Sbjct: 464 LFPYSNPALFSMSLAFLSAWVFSVTDTSERAVEERGRYLGQFIRSMTGIGAAGASKH 520


Lambda     K      H
   0.324    0.138    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 667
Number of extensions: 30
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 551
Length of database: 520
Length adjustment: 35
Effective length of query: 516
Effective length of database: 485
Effective search space:   250260
Effective search space used:   250260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources