Alignments for a candidate for serP in Pseudomonas putida KT2440

GapMind for catabolism of small carbon sources

Alignments for a candidate for serP in Pseudomonas putida KT2440

Align Serine transporter, SerP2 or YdgB, of 459 aas and 12 TMSs (Trip et al. 2013). Transports L-alanine (Km = 20 μM), D-alanine (Km = 38 μM), L-serine, D-serine (Km = 356 μM) and glycine (Noens and Lolkema 2015). The encoding gene is adjacent to the one encoding SerP1 (TC# 2.A.3.1.21) (characterized)
to candidate PP_4840 PP_4840 D-alanine, beta-alanine, D-serine, glycine permease

Query= TCDB::F2HQ24
         (457 letters)



>FitnessBrowser__Putida:PP_4840
          Length = 468

 Score =  362 bits (930), Expect = e-104
 Identities = 189/438 (43%), Positives = 276/438 (63%), Gaps = 6/438 (1%)

Query: 13  QRGLKNRHIQLIAIAGTIGTGLFLGAGKSIHLTGPSIIFVYLIIGALMYILLRAIGEMLY 72
           QR L NRHIQLIAI G IGTGLF+G+GK+I L GPSIIFVY+IIG +++ ++RA+GE+L 
Sbjct: 15  QRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLL 74

Query: 73  QDPNQHSFLNFVSRYLGEKPGYFIQWSYLLVVVFVAMAELIAIGTYINFWLPDLPIWMTE 132
            + N  SF++F +  LG   GYF  W+Y    V   +A+++AI  Y  FW PDLP W+  
Sbjct: 75  SNLNYKSFIDFSADLLGPWAGYFTGWTYWFCWVVTGIADVVAIAAYTQFWFPDLPQWIPA 134

Query: 133 VFVLVLLTLLNTLNPKFFGETEFWFGMIKIVAIIGLILTAIILIFSHYHTGTD-TVSVTN 191
           +  + LL  LN +  K FGE EFWF ++KIVAI+GL+ T + ++ + + + +  T  + N
Sbjct: 135 LTCVGLLLSLNLVTVKMFGELEFWFALVKIVAILGLVATGLYMVITGFTSPSGRTAQLAN 194

Query: 192 ITKGFEFFPNGLSNFFESFQMVMFAFVSMEFIGMTAAETDNPRPTLKKAINQIPIRIVLF 251
           +      FP+GL  FF  FQ+ +FAFV +E +G TAAE  NP  TL +AIN IPIRI++F
Sbjct: 195 LWNDGGMFPHGLMGFFAGFQIAVFAFVGIELVGTTAAEAKNPERTLPRAINSIPIRIIVF 254

Query: 252 YVGALLAIMSIYQWRDIPADKSPFVTIFQLIGIKWAAALVNFVVLTSAASALNSALFSIT 311
           YV AL+AIM++  WRD+   KSPFV +F L G+  AA+++NFVVLTSAAS+ NS +FS +
Sbjct: 255 YVLALIAIMAVTPWRDVVPGKSPFVELFVLAGLPAAASIINFVVLTSAASSANSGVFSTS 314

Query: 312 RNLYSLSKLNNDKILKPFTKFSKAGVPVNALLFT-SLLILFTPFISMIPAISNSFVFITS 370
           R LY LS+  +    K F K S   VP N L F+ + L+L    I ++P +  +F  +T+
Sbjct: 315 RMLYGLSQEGDAP--KAFEKLSSRSVPANGLYFSCTCLLLGAVLIYLVPNVVEAFTLVTT 372

Query: 371 VATNLFLVVYLMTLITYLKYRK--SSDFDPKGFVLPAAHIFIPLAIAGFVLIFISLFCFK 428
           V+  LF+ V+ + L++YLKYRK  ++      + +P       + ++ F  I + L    
Sbjct: 373 VSAVLFMFVWTLILLSYLKYRKDRAALHQASNYKMPGGRFMCYVCLSFFAFILVLLSLEA 432

Query: 429 DTIVPAIGSVIWVLIFGL 446
           DT    + + IW ++  +
Sbjct: 433 DTRSALVVTPIWFVVLAV 450


Lambda     K      H
   0.330    0.144    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 605
Number of extensions: 30
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 457
Length of database: 468
Length adjustment: 33
Effective length of query: 424
Effective length of database: 435
Effective search space:   184440
Effective search space used:   184440
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources