GapMind for catabolism of small carbon sources

 

Aligments for a candidate for scrK in Pseudomonas putida KT2440

Align Fructokinase-1; Fructokinase I; OsFKI; EC 2.7.1.4 (characterized)
to candidate PP_3378 PP_3378 putative 2-ketogluconokinase

Query= SwissProt::Q0JGZ6
         (323 letters)



>lcl|FitnessBrowser__Putida:PP_3378 PP_3378 putative
           2-ketogluconokinase
          Length = 316

 Score =  126 bits (317), Expect = 6e-34
 Identities = 101/320 (31%), Positives = 153/320 (47%), Gaps = 13/320 (4%)

Query: 8   VVSFGEMLIDFVPTVAGVSLAEAPAFVKAPGGAPANVAIAVARLGGGAAFVGKLGDDEFG 67
           V+ FGE +  FV   AG  LA    F K   GA +NVAI +ARLG    ++ ++GDD  G
Sbjct: 6   VLCFGETMAMFVAEQAG-DLAGVGQFGKRIAGADSNVAIGLARLGFKVRWLSRVGDDSLG 64

Query: 68  RMLAAILRDNGVDDGGVVFDAGARTALAFVTLRADG-EREFMFYRNPSADMLLTHAELNV 126
           R +   LR  G+D  GV  DA   T         DG +    ++R  SA   L+ A ++ 
Sbjct: 65  RFVLDSLRCEGLDCSGVEVDANYPTGFQLKARSEDGSDPAVEYFRRGSAASRLSAAMVSP 124

Query: 127 ELIKRAAVFHYGSISLIAEPCRSAHLRAMEIAKEAGALLSYDPNLREALWPSREEARTKI 186
             ++   V   G    +++ CR+     ++  + AG  +S+DPNLR +LWP +     +I
Sbjct: 125 VWLQARHVHATGIPLALSDSCRALSHALLDGMRAAGRSISFDPNLRPSLWPDQSSMVREI 184

Query: 187 LSIWDQADIVKVSEVELEFLTGIDSVEDDVVMKLWRPTMKLLLVTLGDQGCKYYARDFRG 246
            ++  +AD V     E   LTG  +  D     L    ++L+++ LGD G  + +    G
Sbjct: 185 NALAAKADWVLPGLEEGRLLTGQHTPADIAAFYL-DQGVELVVIKLGDAGAYFRSAKGEG 243

Query: 247 AVPSYKVQQ-VDTTGAGDAFVGALLRRIVQDPSSLQDQKKLEEAIKFANACGAITATKKG 305
            V    V + VDT GAGDAF   +L       S+L + + + EA+   N CG+     +G
Sbjct: 244 QVAPVPVSRVVDTVGAGDAFAVGVL-------SALLEGRPVAEAVARGNWCGSRAVQSRG 296

Query: 306 AIPSLPT--EVEVLKLMESA 323
            +  LP   E+E   L  SA
Sbjct: 297 DMEGLPLRHELEAYDLRRSA 316


Lambda     K      H
   0.320    0.136    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 237
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 323
Length of database: 316
Length adjustment: 28
Effective length of query: 295
Effective length of database: 288
Effective search space:    84960
Effective search space used:    84960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory