Align chlorocatechol 1,2-dioxygenase 2 monomer (characterized)
to candidate PP_4656 PP_4656 protocatechuate 3,4-dioxygenase beta chain
Query= metacyc::MONOMER-14665 (254 letters) >FitnessBrowser__Putida:PP_4656 Length = 239 Score = 80.1 bits (196), Expect = 4e-20 Identities = 46/118 (38%), Positives = 67/118 (56%), Gaps = 17/118 (14%) Query: 100 GDTLIFRGEVVDQEGAPLADVLLDMWQADAAGEY-----SFINPTLPDYLFRGKIRTDEN 154 G+ +I G VVDQ G P+ + L++MWQA+A G Y ++ P P++ G+ TD + Sbjct: 76 GERIIVAGRVVDQYGKPVPNTLVEMWQANAGGRYRHKNDRYLAPLDPNFGGVGRCLTDRD 135 Query: 155 GRFTLRTIVPAPYEIPKNGPTGALLAAAGWHAWRPAHLHWIIAKEGYES-LTTQLYFE 211 G ++ RTI P PY +NGP + WRPAH+H+ I+ + L TQLYFE Sbjct: 136 GYYSFRTIKPGPYPW-RNGP----------NDWRPAHIHFAISGPSIATKLITQLYFE 182 Lambda K H 0.316 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 173 Number of extensions: 5 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 254 Length of database: 239 Length adjustment: 24 Effective length of query: 230 Effective length of database: 215 Effective search space: 49450 Effective search space used: 49450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory