GapMind for catabolism of small carbon sources

 

L-tyrosine catabolism in Pseudomonas putida KT2440

Best path

aroP, HPD, hmgA, maiA, fahA, atoA, atoD, atoB

Also see fitness data for the top candidates

Rules

Overview: Tyrosine utilization in GapMind is based on MetaCyc pathway tyrosine degradation I, via homogentisate (link). This pathway requires oxygen. Another pathway via 4-hydroxyphenylacetate is known (link), but the 4-hydroxyphenylpyruvate oxidase has not been linked to sequence. The other MetaCyc pathways do not yield fixed carbon or are not reported in prokaryotes.

19 steps (15 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
aroP L-tyrosine transporter (AroP/FywP) PP_4495 PP_0927
HPD 4-hydroxyphenylpyruvate dioxygenase PP_3433 PP_2554
hmgA homogentisate dioxygenase PP_4621
maiA maleylacetoacetate isomerase PP_4619 PP_1320
fahA fumarylacetoacetate hydrolase PP_4620 PP_3205
atoA acetoacetyl-CoA transferase, A subunit PP_3122 PP_3951
atoD acetoacetyl-CoA transferase, B subunit PP_3123 PP_3952
atoB acetyl-CoA C-acetyltransferase PP_2215 PP_4636
Alternative steps:
aacS acetoacetyl-CoA synthetase PP_3071 PP_3458
Ac3H11_1692 L-tyrosine ABC transporter, ATPase component 2 PP_1137 PP_4863
Ac3H11_1693 L-tyrosine ABC transporter, ATPase component 1 PP_1138 PP_2767
Ac3H11_1694 L-tyrosine ABC transporter, permease component 2 PP_1139 PP_4865
Ac3H11_1695 L-tyrosine ABC transporter, permease component 1 PP_4866 PP_1140
Ac3H11_2396 L-tyrosine ABC transporter, substrate-binding component component PP_4867 PP_1141
CAT L-tyrosine transporter CAT
MCT10 L-tyrosine transporter MCT10
TAT1 L-tyrosine permease TAT1 PP_3727
tyrP Tyrosine permease
tyt1 L-tyrosine:Na+ symporter Tyt1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory