GapMind for catabolism of small carbon sources

 

Protein 6938708 in Shewanella amazonensis SB2B

Annotation: Sama_2811 glycerate dehydrogenase (RefSeq)

Length: 317 amino acids

Source: SB2B in FitnessBrowser

Candidate for 11 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arabinose catabolism gyaR lo Glyoxylate reductase; EC 1.1.1.26 (uncharacterized) 39% 91% 190.7 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
D-xylose catabolism gyaR lo Glyoxylate reductase; EC 1.1.1.26 (uncharacterized) 39% 91% 190.7 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
D-lactate catabolism D-LDH lo D-lactate dehydrogenase (EC 1.1.1.28) (characterized) 36% 78% 151.8 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
L-threonine catabolism D-LDH lo D-lactate dehydrogenase (EC 1.1.1.28) (characterized) 36% 78% 151.8 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
D-cellobiose catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
D-gluconate catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
D-glucose catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
lactose catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
D-maltose catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
sucrose catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0
trehalose catabolism kguD lo 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43) (characterized) 33% 89% 134.4 Hydroxypyruvate reductase; EC 1.1.1.81 45% 270.0

Sequence Analysis Tools

View 6938708 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MTAICVLDGFTLNPGDLDWSPLADIGPFDVHDRTPKELILSRAAGARFLLTNKTVLDAAT
LEAMPSLEYIGVLATGTNVVDVAAAKRLGIAVTNVPGYGPDAVAQMGFAHVLHHMSRVSA
HHDAVTQGQWSGQADFCFTLGQLESLSGKTLGLVGFGDIARQMARIGMAFGMKLLVHSRS
KPTDLPEGAKFVALDTLFAESDVLSLHCPLTDDTRDMVNRESLALMKQGALLINTARGGL
VDEVALADALNAGRVRAGVDVLSTEPPAPNNPLLHAANISITPHNAWATVKARQKLLDIA
VENLRAFSRGESVNRVD

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory