Protein 6939439 in Shewanella amazonensis SB2B
Annotation: FitnessBrowser__SB2B:6939439
Length: 370 amino acids
Source: SB2B in FitnessBrowser
Candidate for 39 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| putrescine catabolism | potA | lo | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) | 40% | 61% | 177.6 | ABC-type molybdate transporter (EC 7.3.2.5) | 30% | 174.1 |
| D-maltose catabolism | thuK | lo | Trehalose/maltose import ATP-binding protein MalK; EC 7.5.2.1 (characterized) | 40% | 63% | 168.7 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| trehalose catabolism | thuK | lo | Trehalose/maltose import ATP-binding protein MalK; EC 7.5.2.1 (characterized) | 40% | 63% | 168.7 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| L-arabinose catabolism | xacK | lo | Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale) | 41% | 59% | 162.2 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-cellobiose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 40% | 63% | 157.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-glucose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 40% | 63% | 157.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| lactose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 40% | 63% | 157.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 40% | 63% | 157.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| sucrose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 40% | 63% | 157.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| trehalose catabolism | gtsD | lo | Sugar ABC transporter ATP-binding protein (characterized, see rationale) | 40% | 63% | 157.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-glucosamine (chitosamine) catabolism | SM_b21216 | lo | ABC transporter for D-Glucosamine, ATPase component (characterized) | 40% | 65% | 157.5 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| L-fucose catabolism | SM_b21106 | lo | ABC transporter for L-Fucose, ATPase component (characterized) | 41% | 58% | 156.8 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| xylitol catabolism | HSERO_RS17020 | lo | ABC-type sugar transport system, ATPase component protein (characterized, see rationale) | 32% | 82% | 154.5 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 40% | 65% | 152.5 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 40% | 65% | 152.5 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| L-proline catabolism | opuBA | lo | BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport (characterized) | 37% | 57% | 151.8 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-cellobiose catabolism | msiK | lo | MsiK protein, component of The cellobiose/cellotriose (and possibly higher cellooligosaccharides), CebEFGMsiK [MsiK functions to energize several ABC transporters including those for maltose/maltotriose and trehalose] (characterized) | 32% | 79% | 151.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | malK1 | lo | MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized) | 35% | 67% | 150.2 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| lactose catabolism | lacK | lo | ABC transporter for Lactose, ATPase component (characterized) | 40% | 60% | 149.8 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | malK_Bb | lo | ABC-type maltose transport, ATP binding protein (characterized, see rationale) | 41% | 62% | 149.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| L-arabinose catabolism | xacJ | lo | Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) | 39% | 54% | 149.1 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| L-arabinose catabolism | araV | lo | AraV, component of Arabinose, fructose, xylose porter (characterized) | 30% | 89% | 146.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-fructose catabolism | araV | lo | AraV, component of Arabinose, fructose, xylose porter (characterized) | 30% | 89% | 146.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | musK | lo | ABC-type maltose transporter (EC 7.5.2.1) (characterized) | 36% | 67% | 146.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| sucrose catabolism | araV | lo | AraV, component of Arabinose, fructose, xylose porter (characterized) | 30% | 89% | 146.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-xylose catabolism | araV | lo | AraV, component of Arabinose, fructose, xylose porter (characterized) | 30% | 89% | 146.4 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | malK_Aa | lo | ABC-type maltose transporter (EC 7.5.2.1) (characterized) | 40% | 57% | 144.8 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| xylitol catabolism | Dshi_0546 | lo | ABC transporter for Xylitol, ATPase component (characterized) | 36% | 66% | 144.1 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-mannitol catabolism | mtlK | lo | SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized) | 38% | 70% | 143.3 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-cellobiose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-glucose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| lactose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | aglK | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| D-maltose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| sucrose catabolism | aglK | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| sucrose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| trehalose catabolism | aglK | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| trehalose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 35% | 70% | 141 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
| trehalose catabolism | treV | lo | TreV, component of Trehalose porter (characterized) | 33% | 81% | 132.9 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 40% | 177.6 |
Sequence Analysis Tools
View 6939439 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
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Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
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Sequence
MSGSTELRVQIRQTKGIRIEADFSCRAGEFLAVVGPSGGGKTTLLRMIAGLAKPENGSIR
CGKRIWFDSDEGIHCSPQNRHIGFVPQHFGLFPKLSALGNIMAALDHLPSQERRPRALAA
LEKVNLHGLTDRLPSQLSGGQKQRVALARALAREPRVLLLDEPFSAVDRETRERLYLELA
RLKAELNIPVIMVTHDLNEALLLADSMLLISQGHMLQHGTPQDVFSRPRNEAVARQMGLR
NIFNAHVVAQDCAKGVTWLKFGDRLIASDSLPRVNIGDKVRWVIPNQGIRFNSIASGRLC
RSFNILPIHIDNMLTLGESVRLEASVQDSGERLNVEIPLHLAHKLALTQGAATEVALKSD
QVHILEMIKA
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory