Aligments for a candidate for acs in Shewanella amazonensis SB2B

GapMind for catabolism of small carbon sources

Aligments for a candidate for acs in Shewanella amazonensis SB2B

Align Acyl-CoA synthetase short-chain family member 3, mitochondrial; Acetate--CoA ligase 3; Acyl-CoA synthetase short-chain family member 3; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 (characterized)
to candidate 6937194 Sama_1364 acyl-coenzyme A synthetase/AMP-(fatty) acid ligase (RefSeq)

Query= SwissProt::Q9H6R3
         (686 letters)



>lcl|FitnessBrowser__SB2B:6937194 Sama_1364 acyl-coenzyme A
           synthetase/AMP-(fatty) acid ligase (RefSeq)
          Length = 644

 Score =  660 bits (1702), Expect = 0.0
 Identities = 315/612 (51%), Positives = 425/612 (69%), Gaps = 2/612 (0%)

Query: 72  DPERFWGKAAEQISWYKPWTKTLENKHSPSTRWFVEGMLNICYNAVDRHIENGKGDKIAI 131
           +PE++W KAA+ + W+K  +  L++  +P  RW+ +G +N CYNA+DR ++ G+G + A 
Sbjct: 25  NPEQYWRKAADMVDWHKAPSTILDSSQAPLYRWYTDGEINTCYNALDRQVDAGRGSQTAF 84

Query: 132 IYDSPVTNTKATFTYKEVLEQVSKLAGVLVKHGIKKGDTVVIYMPMIPQAMYTMLACARI 191
            Y SPVT T+ + +Y E L QV +LAG+L   G+ KGD VVIYMPM+P+  Y MLACARI
Sbjct: 85  HYVSPVTETEYSISYTECLAQVKRLAGLLKAQGVNKGDRVVIYMPMVPETAYAMLACARI 144

Query: 192 GAIHSLIFGGFASKELSSRIDHVKPKVVVTASFGIEPGRRVEYVPLVEEALKIGQHKPDK 251
           GAIHS++FGGFA+ EL+SRI+  KP +V++AS G+EP   V Y PL++ AL    HK  K
Sbjct: 145 GAIHSVVFGGFAANELASRINDAKPVMVLSASCGVEPSGVVAYKPLLDAALAQASHKVSK 204

Query: 252 ILIYNRPNMEAVPLAPGRDLDWDEEMAKAQSHDCVPVLSEHPLYILYTSGTTGLPKGVIR 311
            LI  RP  EA  + PGRDLDW E +A A   D VPV +  PLY+LYTSGTTG PKGV+R
Sbjct: 205 CLILQRPQYEAA-MTPGRDLDWQESVASAADADWVPVKATDPLYVLYTSGTTGQPKGVVR 263

Query: 312 PTGGYAVMLHWSMSSIYGLQPGEVWWAASDLGWVVGHSYICYGPLLHGNTTVLYEGKPVG 371
             GG+AV L WSM  +Y ++PG+ +WAASD+GWVVGHSYI YGPL+ G T+VLYEGKPVG
Sbjct: 264 DNGGHAVALAWSMRYLYDIEPGDTFWAASDVGWVVGHSYIVYGPLIAGATSVLYEGKPVG 323

Query: 372 TPDAGAYFRVLAEHGVAALFTAPTAIRAIRQQDPGAALGKQYSLTRFKTLFVAGERCDVE 431
           TPDAGA++R + ++GV + FTAPTAIRAI+++DP     +  +L   K +F+AGERCD +
Sbjct: 324 TPDAGAFWRTIKKYGVKSFFTAPTAIRAIKREDPEGFFCRNTNLDCLKQMFLAGERCDPD 383

Query: 432 TLEWSKNVFRVPVLDHWWQTETGSPITASCVGLGNSKTPPPGQAGKSVPGYNVMILDDNM 491
           TL W++ +   PV+DHWWQTETG P+ ++ +G+       PG   + VPGY V ++D+  
Sbjct: 384 TLLWAQEMLGKPVIDHWWQTETGWPVASNLMGIAQMPI-KPGSPARPVPGYQVDVVDEAG 442

Query: 492 QKLKARCLGNIVVKLPLPPGAFSGLWKNQEAFKHLYFEKFPGYYDTMDAGYMDEEGYLYV 551
             + A   GN+V++LPLPPG    LW+N + +   Y   +P  Y T DAGYMDE+GYLY+
Sbjct: 443 NSVGAGEYGNVVIRLPLPPGTLPTLWQNDKRYIDSYLSMYPDCYLTGDAGYMDEDGYLYI 502

Query: 552 MSRVDDVINVAGHRISAGAIEESILSHGTVADCAVVGKEDPLKGHVPLALCVLRKDINAT 611
           MSR+DDVINVAGHR+S G  EE +  H  VA+ AV+G ED LKG VPL L VL+  ++ T
Sbjct: 503 MSRIDDVINVAGHRLSTGRFEEVLCQHPAVAEAAVIGVEDKLKGQVPLGLVVLKNGVDIT 562

Query: 612 EEQVLEEIVKHVRQNIGPVAAFRNAVFVKQLPKTRSGKIPRSALSAIVNGKPYKITSTIE 671
           +EQ+ +E+V  VR  IGPVAAF+    + +LPKTRSGKI R+ +  I + + Y + +TIE
Sbjct: 563 DEQLNKELVALVRSEIGPVAAFKLVSAIPKLPKTRSGKILRATMRKIADNQEYSVPATIE 622

Query: 672 DPSIFGHVEEML 683
           DP+    V   L
Sbjct: 623 DPATLELVRNTL 634


Lambda     K      H
   0.318    0.136    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1250
Number of extensions: 60
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 686
Length of database: 644
Length adjustment: 38
Effective length of query: 648
Effective length of database: 606
Effective search space:   392688
Effective search space used:   392688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources