Align The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up (characterized)
to candidate 6937149 Sama_1319 sodium:dicarboxylate symporter (RefSeq)
Query= TCDB::P96603 (421 letters) >FitnessBrowser__SB2B:6937149 Length = 437 Score = 237 bits (604), Expect = 6e-67 Identities = 130/413 (31%), Positives = 233/413 (56%), Gaps = 22/413 (5%) Query: 7 LTVQVITAVIIGVIVGLVWPDV--GKE----------MKPLGDTFINAVKMVIAPIIFFT 54 LT +++ + G+++GL+ + G E +G FIN++KM++ P++F + Sbjct: 11 LTGKILIGMGAGILIGLLLRNFFGGSEWVQDYITEGFFHVIGTIFINSLKMLVVPLVFIS 70 Query: 55 IVLGIAKMGDMKKVGKVGGKAFIYFEVVTTLALIIGLFVVNIMKPGAGLDYSKLEKGDVS 114 +V G + + K+G++GGK ++ T +AL++ + +++PG S + Sbjct: 71 LVCGTCSLSEPSKLGRLGGKTLAFYLFTTAIALVVAISAAVLVQPGNASLAS-----ESM 125 Query: 115 QYTQNGGQGIDWIEFITHIVPSNMVDAFAKGDILQVLFFSILFGVGLAALGEKGKSVIDF 174 QY+ + + + +IVPSN + A ++G++LQ++ F+++FG ++ +GE+G+ V Sbjct: 126 QYSAKEAPSL--ADVLINIVPSNPMKALSEGNMLQIIIFAVIFGFAISHIGERGRRVAAL 183 Query: 175 FDKVSHVFFKIIGYIMRAAPIGAFGAMAYTIGHFGLDSIKPLASLMMSVYITMFLFVFVA 234 FD ++ V +++ IM+ AP G F M G+++++ + M V + + FV Sbjct: 184 FDDLNEVIMRVVTLIMQLAPYGVFALMGKLALTLGMETLESVIKYFMLVLVVLLFHGFVV 243 Query: 235 LNIICKLY-GFSLWNYLRFIKDELLIVLGTSSSESVLPRMMDKME-RYGCSKSVVGLVIP 292 + KL+ G S ++R ++D L T+SS + LP M+ E R G V +P Sbjct: 244 YPTLLKLFSGLSPLMFIRKMRDVQLFAFSTASSNATLPVTMEASEHRLGADNKVASFTLP 303 Query: 293 TGYSFNLDGTSIYLSMATVFLAQVFGVDLSIGQQITIILVLMLTSKGAAGVTGSGFIVLA 352 G + N+DGT+I +ATVF+AQVFG+DL+I +++ L S G AGV G G ++LA Sbjct: 304 LGATINMDGTAIMQGVATVFIAQVFGIDLTITDYAMVVMTATLASIGTAGVPGVGLVMLA 363 Query: 353 STLSALQVIPLEGLALLLGVDRFMSEGRAIVNLIGNGIATIIVAKSENEFDEA 405 L+ + +P+EG+AL+LGVDR + R VN+ G+ +AT+++AKSE +EA Sbjct: 364 MVLNQVG-LPVEGIALILGVDRMLDMVRTAVNVTGDTVATVVIAKSEGALNEA 415 Lambda K H 0.326 0.143 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 437 Number of extensions: 16 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 421 Length of database: 437 Length adjustment: 32 Effective length of query: 389 Effective length of database: 405 Effective search space: 157545 Effective search space used: 157545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory