Aligments for a candidate for adh in Shewanella amazonensis SB2B

GapMind for catabolism of small carbon sources

Aligments for a candidate for adh in Shewanella amazonensis SB2B

Align Aldehyde dehydrogenase (EC 1.2.1.3) (characterized)
to candidate 6939342 Sama_3435 aldehyde dehydrogenase (RefSeq)

Query= reanno::Marino:GFF3202
         (533 letters)



>FitnessBrowser__SB2B:6939342
          Length = 506

 Score =  786 bits (2031), Expect = 0.0
 Identities = 370/506 (73%), Positives = 433/506 (85%)

Query: 28  MIYAQPGKDGSVVSFKSRYENYIGGEWVAPVKGQYFENITPVTGNVICEIPRSSAEDIDL 87
           MIY+ PG  G++ SFK+RY+N+IGG+WV PV G+YF+N++PV G V C+  RS   DI+L
Sbjct: 1   MIYSAPGTAGAIASFKARYDNFIGGKWVPPVGGEYFDNVSPVDGKVFCQAARSDYRDIEL 60

Query: 88  ALDAAHKAAPAWGKTSPTERSNILLKIADRIEANLEKLAVAETWDNGKAVRETLNADIPL 147
           ALDAAH A  +WGKTS TERSN+LLKIADR+E +LE+LAV ETW+NGKAVRETLNAD+PL
Sbjct: 61  ALDAAHAAKDSWGKTSVTERSNLLLKIADRVEQHLERLAVVETWENGKAVRETLNADLPL 120

Query: 148 AADHFRYFAGCLRAQEGHMGEIDHNTVAYHFHEPLGVVGQIIPWNFPILMAAWKLGPCLA 207
             DHFRYFAGC+RAQEG   ++D NTV+YH  EPLGVVGQIIPWNFP+LMAAWK+ P LA
Sbjct: 121 FVDHFRYFAGCIRAQEGSAADLDANTVSYHLPEPLGVVGQIIPWNFPLLMAAWKIAPALA 180

Query: 208 AGNCTVLKPAEQTPASILVLMEIIGDLLPPGVLNIVNGYGIEAGQALATSKRIAKIAFTG 267
           AGNC VLKPAEQTPASI+VL+E I DLLPPGVLN+VNG+G EAG ALATSKRIAK+AFTG
Sbjct: 181 AGNCVVLKPAEQTPASIMVLLETIEDLLPPGVLNVVNGFGAEAGAALATSKRIAKLAFTG 240

Query: 268 STPVGSHILKCAAENIIPSTVELGGKSPNIYFSDVMKAEPEFIDKCVEGLVLAFFNQGEV 327
           ST VG+HILKCAAEN+IPSTVELGGKSPNIYF+DVM  E  ++DK +EG++LAFFNQGEV
Sbjct: 241 STEVGNHILKCAAENLIPSTVELGGKSPNIYFADVMNHEDNYLDKAIEGMLLAFFNQGEV 300

Query: 328 CTCPSRALVQEDMFEEFMQKVVQRTKSIKRGNPLDTDVQVGAQASKEQFDKIMSYLAIGK 387
           CTCPSR LVQE +++ F++KV+ R K+I++G+PLDTD QVGAQAS+EQ+DKI+ YL IG+
Sbjct: 301 CTCPSRVLVQESIYDAFIEKVIARAKTIRQGSPLDTDTQVGAQASREQYDKILGYLDIGR 360

Query: 388 EEGAVVLTGGDREHLDEEFNNGFYIQPTLFKGDNKMRVFQEEIFGPVVGVTTFKTEEEAL 447
            EGA VL GG+ +  D     G+YIQPT+ KG NKMRVFQEEIFGPVV VTTFK E EAL
Sbjct: 361 AEGAKVLMGGEFKLQDGPEKGGYYIQPTILKGHNKMRVFQEEIFGPVVSVTTFKDEAEAL 420

Query: 448 AIANDTEFGLGAGVWTRDTNLAYRMGRNIQAGRVWMNCYHAYPAHAAFGGYKKSGVGRET 507
           AIANDT++GLGAGVWTRD NLA RMGR IQAGRVW+NCYHAYPAHAAFGGYKKSG+GRET
Sbjct: 421 AIANDTQYGLGAGVWTRDMNLAQRMGRGIQAGRVWINCYHAYPAHAAFGGYKKSGIGRET 480

Query: 508 HKMALEHYQQTKCMLTSYDTNPLGFF 533
           HKM L HYQ TK +L S+DTNPLGFF
Sbjct: 481 HKMMLSHYQNTKNLLVSFDTNPLGFF 506


Lambda     K      H
   0.319    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 853
Number of extensions: 32
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 533
Length of database: 506
Length adjustment: 35
Effective length of query: 498
Effective length of database: 471
Effective search space:   234558
Effective search space used:   234558
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources