Align phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) (EC 5.4.2.2) (characterized)
to candidate 6937550 Sama_1706 phosphoglucomutase (RefSeq)
Query= BRENDA::A0A0H3NJ17 (546 letters) >FitnessBrowser__SB2B:6937550 Length = 560 Score = 648 bits (1671), Expect = 0.0 Identities = 322/549 (58%), Positives = 401/549 (73%), Gaps = 5/549 (0%) Query: 1 MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGTSGHRGSAGRHSFNEPH 60 MAIH+RAG+ AQQ DL+N+ +L + YY L P+A + V FGTSGHRG A SFNE H Sbjct: 1 MAIHDRAGKQAQQGDLVNIPKLMSHYYRLLPDASDPAQKVSFGTSGHRGCAFHRSFNEQH 60 Query: 61 ILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVLAANGVDVIVQENNGFTPT 120 ILAIAQA+ + R + ITGP Y+G DTHALS+ A++SV+EVL AN V V+ Q ++ +TPT Sbjct: 61 ILAIAQAVVDWRTQADITGPLYLGMDTHALSQAAYLSVIEVLIANKVQVLAQRDDAYTPT 120 Query: 121 PAVSNAILVHNK----KGGPLADGIVITPSHNPPEDGGIKYNPPNGGPADTNVTKVVEDR 176 P VS AI+ N+ + L+DGI+ITPSHNPP+DGGIKYNPP+GGPA+ ++T +E R Sbjct: 121 PVVSQAIVAANRHQTGEDALLSDGIIITPSHNPPQDGGIKYNPPHGGPAEGDITSWIETR 180 Query: 177 ANALLAGGLQGVKRISLDAAMASGHVKAVDLVQPFVEGLADIVDMAAIQKAGLTLGVDPL 236 AN LA GL GV+R++ A +K VDL+ P+V+ LA+++DMAAI KAG+ +GVDPL Sbjct: 181 ANHYLAHGLDGVRRVNYGIASGLSLLKHVDLISPYVQSLAEVIDMAAIAKAGVRMGVDPL 240 Query: 237 GGSGIEYWKRIAEHYKLNLTLVNDQVDQTFRFMHLDKDGAIRMDCSSECAMAGLLALRDK 296 GGSGI YW+ IAEHY LN+ +VN +D TF FM LDKDG IRMDCSS AMAGLL +D+ Sbjct: 241 GGSGIHYWQPIAEHYGLNIEVVNQAIDPTFDFMPLDKDGKIRMDCSSPYAMAGLLVHKDE 300 Query: 297 FDLAFANDPDYDRHGIVTP-AGLMNPNHYLAVAINYLFQHRPLWGKDVAVGKTLVSSAMI 355 FD+ NDPDYDRHGIV P +GLM+PNHYLAVAI+YL HRP W K +AVGKTLVSSA+I Sbjct: 301 FDICVGNDPDYDRHGIVCPGSGLMDPNHYLAVAIDYLLGHRPNWAKTLAVGKTLVSSALI 360 Query: 356 DRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEESAGASFLRFDGTPWSTDKDGIIMC 415 D+V G L+EVPVGFKWFVDGL D S FGGEESAGA+FL+ DG+ W TDKDG I+ Sbjct: 361 DKVCMAHGVPLMEVPVGFKWFVDGLADSSVCFGGEESAGAAFLKMDGSTWCTDKDGFILA 420 Query: 416 LLAAEITAVTGKNPQEHYNELAARFGAPSYNRLQASATSAQKAALSKLSPEMVSASTLAG 475 LLAAEI AVTGK P E Y EL A G Y R+ + KA L+ + + A+++AG Sbjct: 421 LLAAEILAVTGKTPAERYEELVAEHGRSFYKRIDSPLNPKMKAKFGALNAQTLGATSVAG 480 Query: 476 DPITARLTAAPGNGASIGGLKVMTDNGWFAARPSGTEDAYKIYCESFLGEEHRKQIEKEA 535 D ITA LT APGNGA+IGG+KV TD GWFAARPSGTE +K+Y ESF+ E H ++ +A Sbjct: 481 DAITAVLTKAPGNGAAIGGIKVCTDKGWFAARPSGTEPLFKLYAESFVSEAHLGELISDA 540 Query: 536 VEIVSEVLK 544 ++ LK Sbjct: 541 QSLLGTALK 549 Lambda K H 0.316 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 913 Number of extensions: 31 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 546 Length of database: 560 Length adjustment: 36 Effective length of query: 510 Effective length of database: 524 Effective search space: 267240 Effective search space used: 267240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate 6937550 Sama_1706 (phosphoglucomutase (RefSeq))
to HMM TIGR01132 (pgm: phosphoglucomutase, alpha-D-glucose phosphate-specific (EC 5.4.2.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01132.hmm # target sequence database: /tmp/gapView.20426.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01132 [M=546] Accession: TIGR01132 Description: pgm: phosphoglucomutase, alpha-D-glucose phosphate-specific Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.2e-260 849.1 0.1 7e-260 848.9 0.1 1.0 1 lcl|FitnessBrowser__SB2B:6937550 Sama_1706 phosphoglucomutase (Re Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6937550 Sama_1706 phosphoglucomutase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 848.9 0.1 7e-260 7e-260 1 545 [. 1 548 [. 1 549 [. 0.98 Alignments for each domain: == domain 1 score: 848.9 bits; conditional E-value: 7e-260 TIGR01132 1 mainpraGqkaqqedlldvaklvadyyllkpdaenaaqkvefGtsGhrGsalkgtfneahilaiaqavvevraaqGi 77 mai++raG++aqq dl++++kl+++yy+l pda ++aqkv+fGtsGhrG+a + +fne+hilaiaqavv+ r++ i lcl|FitnessBrowser__SB2B:6937550 1 MAIHDRAGKQAQQGDLVNIPKLMSHYYRLLPDASDPAQKVSFGTSGHRGCAFHRSFNEQHILAIAQAVVDWRTQADI 77 89*************************************************************************** PP TIGR01132 78 tGplyiGkdthalsepafvsvlevlaanqvevivqennrytptpavshailtynkgkkea...ladGivitpshnpp 151 tGply+G+dthals+ a+ sv+evl+an+v+v+ q + ytptp vs+ai++ n+ ++++ l+dGi+itpshnpp lcl|FitnessBrowser__SB2B:6937550 78 TGPLYLGMDTHALSQAAYLSVIEVLIANKVQVLAQRDDAYTPTPVVSQAIVAANRHQTGEdalLSDGIIITPSHNPP 154 ***************************************************99986654422278************ PP TIGR01132 152 edGGikynppnGGpaetevtkaiedranellkdrlkgvkrldlekalksetvkekdlvkpyvddladvvdlaairka 228 +dGGikynpp+GGpae+++t +ie ran++l+++l gv+r+++ a +++k++dl++pyv+ la+v+d+aai ka lcl|FitnessBrowser__SB2B:6937550 155 QDGGIKYNPPHGGPAEGDITSWIETRANHYLAHGLDGVRRVNYGIASGLSLLKHVDLISPYVQSLAEVIDMAAIAKA 231 ***************************************************************************** PP TIGR01132 229 glrlGvdplGGagvdywkeiaekynldltlvneavdatfrfmtldkdGkirmdcsspyamagllklkdkydlafgnd 305 g+r+GvdplGG+g++yw++iae+y+l++ +vn+a+d+tf fm ldkdGkirmdcsspyamagll kd++d+ +gnd lcl|FitnessBrowser__SB2B:6937550 232 GVRMGVDPLGGSGIHYWQPIAEHYGLNIEVVNQAIDPTFDFMPLDKDGKIRMDCSSPYAMAGLLVHKDEFDICVGND 308 ***************************************************************************** PP TIGR01132 306 adadrhGivtpdkGllnpnhylavaieylykhrqqwaaevavGktlvssalidrvvadlgrklvevpvGfkwfvdGl 382 +d+drhGiv p +Gl++pnhylavai+yl hr++wa+++avGktlvssalid+v g l+evpvGfkwfvdGl lcl|FitnessBrowser__SB2B:6937550 309 PDYDRHGIVCPGSGLMDPNHYLAVAIDYLLGHRPNWAKTLAVGKTLVSSALIDKVCMAHGVPLMEVPVGFKWFVDGL 385 ***************************************************************************** PP TIGR01132 383 ldgslGfGGeesaGasflrkdGtvwstdkdGiilallaaeitavtGknpqqrydelaakyGdpiyaridaaatsaqk 459 d+s+ fGGeesaGa+fl+ dG++w+tdkdG ilallaaei+avtGk+p +ry+el a++G +y+rid++ +++ k lcl|FitnessBrowser__SB2B:6937550 386 ADSSVCFGGEESAGAAFLKMDGSTWCTDKDGFILALLAAEILAVTGKTPAERYEELVAEHGRSFYKRIDSPLNPKMK 462 ***************************************************************************** PP TIGR01132 460 arlkklspdevsattlaGdaitakltkapGngaaiGGlkvttdegwfaarpsGtedvykiyaesfkgeehlkeieke 536 a++ l ++++ at++aGdaita ltkapGngaaiGG+kv td+gwfaarpsGte ++k+yaesf e+hl e+ ++ lcl|FitnessBrowser__SB2B:6937550 463 AKFGALNAQTLGATSVAGDAITAVLTKAPGNGAAIGGIKVCTDKGWFAARPSGTEPLFKLYAESFVSEAHLGELISD 539 ***************************************************************************** PP TIGR01132 537 aeeivdevl 545 a+ ++ +l lcl|FitnessBrowser__SB2B:6937550 540 AQSLLGTAL 548 **9987776 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (546 nodes) Target sequences: 1 (560 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 8.25 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory