Alignments for a candidate for gltP in Shewanella amazonensis SB2B

GapMind for catabolism of small carbon sources

Alignments for a candidate for gltP in Shewanella amazonensis SB2B

Align Na+/H+ dicarboxylate symporter (characterized, see rationale)
to candidate 6938665 Sama_2768 proton/glutamate symporter (RefSeq)

Query= uniprot:L0GT47
         (419 letters)



>FitnessBrowser__SB2B:6938665
          Length = 430

 Score =  476 bits (1225), Expect = e-139
 Identities = 237/400 (59%), Positives = 311/400 (77%), Gaps = 1/400 (0%)

Query: 17  LPLWQQILIGLALGVAAGMAFGADAQLLAPIGTLFLNAIKMLIVPLVFVSLVAGITSMQD 76
           +PLW QIL G+ LG+  G+  G DA  L PIGTLF+N IKMLIVPLVF SL+ G+TSMQD
Sbjct: 16  VPLWLQILTGMLLGIGVGLVLGPDASALKPIGTLFVNTIKMLIVPLVFCSLIVGVTSMQD 75

Query: 77  SAKLGRISLKTIAIYLVTTAFAVSIGLLFGALFSPGEGMNMVASG-NEQAKQAPSLVSIL 135
           +A++GRI  K+ A YL TTA A+S+GL+ G L  PG G+++     + + K APS++  L
Sbjct: 76  TARMGRIGFKSFAFYLATTAIAISVGLMVGWLLEPGAGLSLEGHDLSAEVKTAPSVMDTL 135

Query: 136 VGLVPANPVTAFAEGNILQIIVFAIALGVSINLIGERGAPAVRLFDALAETFYKLTDLVM 195
           + +VP NPV A A G ILQ+IVFA+ALGV++ LIG+ G PA+++F++LAE  YKLTD+VM
Sbjct: 136 INIVPTNPVAALASGQILQVIVFAVALGVALVLIGDHGKPAIKVFESLAEAMYKLTDMVM 195

Query: 196 RVAPIGVFALTAGVVGSHGAEVLLPLAGVIGVIYLASIAHVLLVYGGLLGLLARLNPLRF 255
           ++AP GVF L A V G +G ++LLPL  VI  +YL    H+L  Y  +L L+A L+P++F
Sbjct: 196 KLAPYGVFGLMAWVAGEYGIDMLLPLIKVIIAVYLGCALHILGFYSLVLKLVAGLSPIQF 255

Query: 256 FQGIAPALAVAFSTSSSSGTLPVSIECARKNLGVSEGVAGFVLPVGATINMDGTAIYQGV 315
           F+GI+ A+AVAF+TSSS+GTLP S++CA + LGV++ ++ FVLP+G TINMDGTA+YQGV
Sbjct: 256 FKGISNAMAVAFTTSSSAGTLPASMKCASEYLGVNKKISSFVLPLGTTINMDGTALYQGV 315

Query: 316 LALFIAQAFGIDLSAGQYAMIILTATLASIGTAGIPGAGLIMLGLVLTAAGLPLEGVALI 375
            ALF+AQAFG+DL+   Y  I+LTATLASIGTAG+PGAGL+ML LVLT  GLPLEGVA+I
Sbjct: 316 TALFVAQAFGVDLTWVDYLTIVLTATLASIGTAGVPGAGLVMLTLVLTTVGLPLEGVAII 375

Query: 376 AGIDRILDMARTTVNVAGDLMTTTLVGRSEQELDRAIYDS 415
           AGIDRILDMART VNV+GDL+ TT++ RSE E+D A Y++
Sbjct: 376 AGIDRILDMARTVVNVSGDLVATTVIARSEGEIDIAHYNA 415


Lambda     K      H
   0.324    0.140    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 652
Number of extensions: 27
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 419
Length of database: 430
Length adjustment: 32
Effective length of query: 387
Effective length of database: 398
Effective search space:   154026
Effective search space used:   154026
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources