Align glycerol kinase; EC 2.7.1.30 (characterized)
to candidate 6936144 Sama_0341 glycerol kinase (RefSeq)
Query= CharProtDB::CH_121461 (502 letters) >FitnessBrowser__SB2B:6936144 Length = 495 Score = 718 bits (1853), Expect = 0.0 Identities = 342/494 (69%), Positives = 415/494 (84%), Gaps = 1/494 (0%) Query: 3 EKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPMEIWATQSSTLV 62 +K+Y+VALDQGTTSSRA+V D DAN+++VSQREF QIYPK GWVEHDPMEIWA+QSSTL+ Sbjct: 2 DKQYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTLI 61 Query: 63 EVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLE 122 EVLA+A I SD++AAIGITNQRETTI+W+K TGKP+ NAIVWQCRR+A ICE LK GLE Sbjct: 62 EVLARAGIHSDEVAAIGITNQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGLE 121 Query: 123 DYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT 182 R TGL++DPYFSGTK+KWILD+V G RERA GELLFGTVDTWL+WK+T+G+VHVT Sbjct: 122 PLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADAGELLFGTVDTWLVWKLTEGKVHVT 181 Query: 183 DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISG 242 D TNASRT+++NIH+ +WD ++L+ LDIP +LPEV+ S VYG T I G+G T IP++G Sbjct: 182 DPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIAGEG-TEIPVAG 240 Query: 243 IAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYA 302 +AGDQQAALFGQLCV+ GMAKNTYGTGCF+LMNTG++AVKSE+GLLTTIA G G ++YA Sbjct: 241 MAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGISYA 300 Query: 303 LEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLGAPYWDPYAR 362 LEGAVFM GA+IQWLRDE+ LI DA D+EYFA+KV +TNGVY+VPAF GLGAPYWDP AR Sbjct: 301 LEGAVFMGGATIQWLRDELGLIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDAR 360 Query: 363 GAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQF 422 G + GLTRG N NHIIRA LESIAYQ+RD+L+AM DSG+ L L+VDGGAVAN+FLMQF Sbjct: 361 GTLVGLTRGANRNHIIRAALESIAYQSRDLLDAMSKDSGVALKQLKVDGGAVANDFLMQF 420 Query: 423 QSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIETTERNY 482 Q+DI G V RP E TA+GAA+L GLAVGFW N+DE++ K+ I+R F P ++ ER Sbjct: 421 QADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHKSGIDRRFVPLMDDKERAT 480 Query: 483 RYAGWKKAVKRAMA 496 Y GW+ AV+R+++ Sbjct: 481 LYLGWQDAVRRSLS 494 Lambda K H 0.318 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 815 Number of extensions: 18 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 502 Length of database: 495 Length adjustment: 34 Effective length of query: 468 Effective length of database: 461 Effective search space: 215748 Effective search space used: 215748 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate 6936144 Sama_0341 (glycerol kinase (RefSeq))
to HMM TIGR01311 (glpK: glycerol kinase (EC 2.7.1.30))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01311.hmm # target sequence database: /tmp/gapView.19240.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01311 [M=496] Accession: TIGR01311 Description: glycerol_kin: glycerol kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.9e-226 737.0 0.0 4.4e-226 736.8 0.0 1.0 1 lcl|FitnessBrowser__SB2B:6936144 Sama_0341 glycerol kinase (RefSe Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6936144 Sama_0341 glycerol kinase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 736.8 0.0 4.4e-226 4.4e-226 1 494 [. 4 493 .. 4 495 .] 0.99 Alignments for each domain: == domain 1 score: 736.8 bits; conditional E-value: 4.4e-226 TIGR01311 1 kliaaiDqGttssraivfdkegeevakaqkelsqifpkegwvEhdpleilesvvkvlaealekleikaeeiaaiGit 77 ++++a+DqGttssraivfd++++ va +q+e++qi+pk gwvEhdp+ei++s+ ++l e+l++++i+++e+aaiGit lcl|FitnessBrowser__SB2B:6936144 4 QYVVALDQGTTSSRAIVFDRDANLVAVSQREFTQIYPKGGWVEHDPMEIWASQSSTLIEVLARAGIHSDEVAAIGIT 80 69*************************************************************************** PP TIGR01311 78 nqREttvvWdketgkplvnaivWqdtrtakiveelkeetkeeelrektGLplstYfsatKlrWlldnveevrkaaee 154 nqREtt++Wdk tgkp++naivWq++r a i+e+lk+++ e +r+ktGL+l++Yfs+tK++W+ldnv++vr++a++ lcl|FitnessBrowser__SB2B:6936144 81 NQRETTIIWDKLTGKPVCNAIVWQCRRSAAICEALKAQGLEPLFRDKTGLLLDPYFSGTKIKWILDNVPGVRERADA 157 ***************************************************************************** PP TIGR01311 155 gellfGtvdtwliykLtggkvhvtdvtNASRtlllnletlkwdeellelfkipkellPeirsssevygeieekellk 231 gellfGtvdtwl++kLt+gkvhvtd tNASRtl++n+++ +wd+ell++++ip++llPe++ss++vyg+++ + + lcl|FitnessBrowser__SB2B:6936144 158 GELLFGTVDTWLVWKLTEGKVHVTDPTNASRTLMYNIHSGEWDSELLKALDIPASLLPEVKSSQAVYGTTRIA-GEG 233 *********************************************************************9998.89* PP TIGR01311 232 eevpitgvlGdqqaalvgqlclkkgeaKntYgtGcFlllntGekkviskhglLttvayklggkkptkyalEGsvava 308 +e+p++g++Gdqqaal+gqlc+++g+aKntYgtGcFll+ntG+++v s+hglLtt+a +g +yalEG+v+++ lcl|FitnessBrowser__SB2B:6936144 234 TEIPVAGMAGDQQAALFGQLCVEPGMAKNTYGTGCFLLMNTGKQAVKSEHGLLTTIAVGDDGGI--SYALEGAVFMG 308 *********************************************************9877766..6********** PP TIGR01311 309 GaavqwlrdnlklikkaeeveklaksvedsegvyfVPafsGLfaPyWdsdArgtivGltrkttkehiaraaleavaf 385 Ga++qwlrd+l+li++a+++e +a++v+d++gvy+VPaf GL+aPyWd+dArgt+vGltr+++++hi+raale++a+ lcl|FitnessBrowser__SB2B:6936144 309 GATIQWLRDELGLIRDASDTEYFASKVDDTNGVYLVPAFVGLGAPYWDPDARGTLVGLTRGANRNHIIRAALESIAY 385 ***************************************************************************** PP TIGR01311 386 qardileamekdagvevkvLkvDGglsknnllmqiqadilgvkverpkvaettalGaAlaaglavgvwkseeeleks 462 q+rd+l+am+kd+gv +k+LkvDGg+++n++lmq+qadi gv+v rp ++etta+GaA++ glavg+w++ +e++++ lcl|FitnessBrowser__SB2B:6936144 386 QSRDLLDAMSKDSGVALKQLKVDGGAVANDFLMQFQADISGVEVLRPAQTETTAMGAAFLGGLAVGFWSNVDEIRHK 462 ***************************************************************************** PP TIGR01311 463 aeaeektfepemdeeerekkykkwkeaversl 494 + + ++f p md++er++ y w++av+rsl lcl|FitnessBrowser__SB2B:6936144 463 SGID-RRFVPLMDDKERATLYLGWQDAVRRSL 493 **98.*************************97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (496 nodes) Target sequences: 1 (495 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.08 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory