Align 3-ketoacyl-CoA thiolase [isoleucine degradation] (EC 2.3.1.16) (characterized)
to candidate 6937205 Sama_1375 Acetyl-CoA C-acetyltransferase (RefSeq)
Query= reanno::MR1:200842 (396 letters) >FitnessBrowser__SB2B:6937205 Length = 392 Score = 664 bits (1712), Expect = 0.0 Identities = 341/388 (87%), Positives = 360/388 (92%) Query: 9 EIVIVAAKRTPMGSFQGSLSGITSLSLAATAIKALLADTQVAPDKVDEVLMGCVLPAGLG 68 +IVIVAAKRT MG FQGSLS + S LAATA+KALL DT + +VDE+LMGCVLPAGLG Sbjct: 5 DIVIVAAKRTAMGGFQGSLSEVPSPKLAATAVKALLDDTGLDGARVDELLMGCVLPAGLG 64 Query: 69 QAPARQATLGAGLPLSVGATTVNKVCGSGMKTVMLAHDLIKAGSAKVVIAGGMESMSQAP 128 QAPARQA LGAGLPLSVGATTVNKVCGSGMKTVMLAHDLIKAGSAKVVIAGGMESMSQAP Sbjct: 65 QAPARQAALGAGLPLSVGATTVNKVCGSGMKTVMLAHDLIKAGSAKVVIAGGMESMSQAP 124 Query: 129 YLLDKARAGIRMGHGKVLDHMFLDGLEDAYTGGAMGTFAQKTADEFGITREQMDAFALSS 188 YLLDKAR G+RMGHGKVLDHMFLDGLEDAYTGGAMGTFAQKTAD++G+TRE MDAFALSS Sbjct: 125 YLLDKARGGMRMGHGKVLDHMFLDGLEDAYTGGAMGTFAQKTADDYGLTRESMDAFALSS 184 Query: 189 LEKANAAINSGAFKTEIVPVTVSDRRGDVTIDTDEQPGNARPEKIPTLRPAFAKDGTITA 248 LEKANAAINSGAF+ EIVPVTVS R+GDV + DEQPGNARPEKIPTLRPAFAKDGTITA Sbjct: 185 LEKANAAINSGAFEAEIVPVTVSSRKGDVEVKVDEQPGNARPEKIPTLRPAFAKDGTITA 244 Query: 249 ANSSSISDGAAALMLTTRANAEQLGLTVLATIKGHTTHAQEPALFTTAPVGAMAKLLSNV 308 ANSSSISDGAAALML +R A+ LGL VLATIKGHTTHAQEPA+FTTAPVGAM KLLSNV Sbjct: 245 ANSSSISDGAAALMLMSRDQADALGLKVLATIKGHTTHAQEPAMFTTAPVGAMTKLLSNV 304 Query: 309 GWSKDEVDLFEINEAFAMVTMLAVSELGLDMTKVNVNGGACALGHPIGCSGARLLVTLIH 368 GWSKDEVDLFEINEAFAMVTMLA+SEL LD +VNVNGGACALGHPIGCSGAR+LVTLIH Sbjct: 305 GWSKDEVDLFEINEAFAMVTMLAISELKLDAARVNVNGGACALGHPIGCSGARVLVTLIH 364 Query: 369 ALKARGLKRGVASLCIGGGEATAMAIEV 396 ALKARGLKRGVASLCIGGGEATAMAIEV Sbjct: 365 ALKARGLKRGVASLCIGGGEATAMAIEV 392 Lambda K H 0.316 0.131 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 609 Number of extensions: 11 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 396 Length of database: 392 Length adjustment: 31 Effective length of query: 365 Effective length of database: 361 Effective search space: 131765 Effective search space used: 131765 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate 6937205 Sama_1375 (Acetyl-CoA C-acetyltransferase (RefSeq))
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.11769.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.6e-130 418.9 8.7 1.1e-129 418.7 8.7 1.0 1 lcl|FitnessBrowser__SB2B:6937205 Sama_1375 Acetyl-CoA C-acetyltra Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6937205 Sama_1375 Acetyl-CoA C-acetyltransferase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 418.7 8.7 1.1e-129 1.1e-129 1 385 [] 8 391 .. 8 391 .. 0.98 Alignments for each domain: == domain 1 score: 418.7 bits; conditional E-value: 1.1e-129 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaaglpesvpal 77 iv+a Rt++g ++gsl+e+++ +L+a+++k+ll+ +gld +++de+ +G+vl+ag ++++aR+aal aglp sv a+ lcl|FitnessBrowser__SB2B:6937205 8 IVAAKRTAMGGFQGSLSEVPSPKLAATAVKALLDDTGLDGARVDELLMGCVLPAGLGQAPARQAALGAGLPLSVGAT 84 8*********99***************************************************************** PP TIGR01930 78 tvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakledqllkdl...vktklsm 151 tvn+vC+Sg+++v+la + ikaG a+vv+aGG+EsmS++p+ll+++ r ++++g+ k+ d++ d+ ++t+ +m lcl|FitnessBrowser__SB2B:6937205 85 TVNKVCGSGMKTVMLAHDLIKAGSAKVVIAGGMESMSQAPYLLDKA--RGGMRMGHGKVLDHMFLDGledAYTGGAM 159 ********************************************98..89***********9999999*999***** PP TIGR01930 152 getAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..kkvvskDegirpnttlekLakLkpa 226 g+ A+++a++yg++Re++D++al+S +ka++Ai++g f++eivpv+v + ++ v+ De++ n ek+ +L+pa lcl|FitnessBrowser__SB2B:6937205 160 GTFAQKTADDYGLTRESMDAFALSSLEKANAAINSGAFEAEIVPVTVSSRkgDVEVKVDEQPG-NARPEKIPTLRPA 235 ***********************************************9999888888898876.99*********** PP TIGR01930 227 fkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivsaavagvdpeemglgpvpAiekaLkkaglsisd 303 f++ +g t+tA+Nss+++DGAaal+lms++ a++lgl++la+i ++++ + +p+ ++++pv A++k+L+ g+s ++ lcl|FitnessBrowser__SB2B:6937205 236 FAK-DG-TITAANSSSISDGAAALMLMSRDQADALGLKVLATIKGHTTHAQEPAMFTTAPVGAMTKLLSNVGWSKDE 310 *95.9*.6********************************************************************* PP TIGR01930 304 idlvEinEAFAaqvlavekelgsldlekvNvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGa 380 +dl+EinEAFA ++++++ el+ ld ++vNvnGGA AlGHP+G+sGar+++tl+++Lk rg k+G+a+lC+ggG ++ lcl|FitnessBrowser__SB2B:6937205 311 VDLFEINEAFAMVTMLAISELK-LDAARVNVNGGACALGHPIGCSGARVLVTLIHALKARGLKRGVASLCIGGGEAT 386 **********************.88**************************************************** PP TIGR01930 381 Avile 385 A+ +e lcl|FitnessBrowser__SB2B:6937205 387 AMAIE 391 **987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (392 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 8.39 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory