Align The maltose/maltooligosaccharide transporter, MalI (541 aas) (characterized)
to candidate 6937501 Sama_1657 major facilitator transporter (RefSeq)
Query= TCDB::Q9A612 (541 letters) >FitnessBrowser__SB2B:6937501 Length = 518 Score = 567 bits (1460), Expect = e-166 Identities = 292/497 (58%), Positives = 362/497 (72%), Gaps = 10/497 (2%) Query: 44 LSFLQIWNMCFGFFGIQIGFGLQNANTSRIFQSLGVDVNHLAILWIAAPATGLLVQPIIG 103 LSF QI+NMCFGF GIQ GF LQNAN SRIFQ+LG ++ + ILWIA P TGLLVQPI+G Sbjct: 14 LSFWQIFNMCFGFLGIQFGFALQNANVSRIFQTLGASIDDIPILWIAGPLTGLLVQPIVG 73 Query: 104 HFSDKTWGRFGRRRPYFFWGAILTTLALLVMPNSPTLWVAAAALWIMDASINITMEPFRA 163 + SD TW RFGRRRPYF GA+LTTLAL +MP+SP LWVAA LWIMDASINI MEPFRA Sbjct: 74 YLSDNTWNRFGRRRPYFLIGAVLTTLALFIMPHSPYLWVAAGMLWIMDASINIAMEPFRA 133 Query: 164 FVGDNLPDEQRATGYAMQSFFIGLGAVFASALPWMLTNWFDVANTAPAGQVPDSVRIAFY 223 FVGD LP+ QRA G+AMQSFFIG+GAV ASALP++L+N+ V+N AP GQ+ D+VR +FY Sbjct: 134 FVGDKLPESQRAKGFAMQSFFIGIGAVVASALPYLLSNFAGVSNIAPEGQIADTVRYSFY 193 Query: 224 TGGAGLLLAVLWTVFTTREYSPEQLTAFEKAEREIAGLGLHEEPEPSVNAYIALGVGGVL 283 GG LL AV WT+ ++REYSPE+L+ F+ E+ +G H P AY + Sbjct: 194 LGGVVLLAAVGWTIISSREYSPEELSRFKGHEQ--SGFERHHGRTP--EAYRKGALVWST 249 Query: 284 LGAALALIVWGAGLEKELYVLAGLLFAFG-----LAGVAGARFKRIGRTDNGFSEVLADV 338 LG A+ + A L+K+LY+L +FAFG A A KR G+ F EV+ D+ Sbjct: 250 LGLAITAATFAADLDKQLYLLGLGVFAFGPLQWYCAQRLSAAGKRSGKLGMVF-EVVDDL 308 Query: 339 FRMPKTMRQLAVVQFFSWFGLFAMWIYTTPAVATVHFGAVDASSKAYNEGADWVGVLFAV 398 F MP M++LA+VQFFSWF LFAMWIYTT AV + H+ + D SKAYN+GADWVGVLFA Sbjct: 309 FHMPHAMKRLALVQFFSWFALFAMWIYTTAAVTSYHYHSSDVLSKAYNDGADWVGVLFAS 368 Query: 399 YNGVAALAALVIPLMVKVTSRRVSHAVCLGLGALGLLSFLVIRDPGLLWIGMVGVGFAWS 458 YNG AALAA+VIPL + + +H + L LG +GL+SF +I DP LLWI M+GVGFAW+ Sbjct: 369 YNGFAALAAMVIPLQCMLLGLKGAHIMNLCLGGIGLMSFYLIADPALLWIPMIGVGFAWA 428 Query: 459 SILSTPYSILAGALPARKMGVYMGIFNVFIVVPQLLAATLLGLMLKTFFGNQSIFALVLG 518 SILS PY+IL+ +LP K+GVYMGIFN FIV+PQL+AAT+LG +LK+ FGN+ I+AL+LG Sbjct: 429 SILSVPYAILSTSLPGNKLGVYMGIFNFFIVIPQLVAATVLGFILKSLFGNEPIYALILG 488 Query: 519 ALSFALAAAATFLVRDR 535 + LAA + V R Sbjct: 489 GSAMILAALSVLFVPAR 505 Lambda K H 0.327 0.140 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 808 Number of extensions: 35 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 541 Length of database: 518 Length adjustment: 35 Effective length of query: 506 Effective length of database: 483 Effective search space: 244398 Effective search space used: 244398 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory