Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate 6937211 Sama_1381 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq)
Query= SwissProt::O18404 (255 letters) >FitnessBrowser__SB2B:6937211 Length = 252 Score = 137 bits (344), Expect = 3e-37 Identities = 95/257 (36%), Positives = 136/257 (52%), Gaps = 12/257 (4%) Query: 2 IKNAVSLVTGGASGLGRATAERLAKQGASVILADLPSSKGNEVAKELGD--KVVFVPVDV 59 +K+ V ++TGGA GLG A A+ LA GA + L D+ + ++GD +V +D+ Sbjct: 3 LKDKVIVITGGAGGLGLAMAKDLAAHGAKLALIDVDQERLERACADIGDATEVQGYALDI 62 Query: 60 TSEKDVSAALQTAKDKFGRLDLTVNCAGT---ATAVKTFNFNKNVAHRLEDFQRVININT 116 T E+DV A + + FG + VN AG +K + L+ FQ VIN+N Sbjct: 63 TDEEDVVAGFRYILEDFGVIHGLVNNAGILRDGLLIKAKDGVVTDRMSLDQFQSVINVNL 122 Query: 117 VGTFNVIRLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPI 176 GTF R +A M + GQ GVIVN +S+A G +GQ Y+ASKA V M + Sbjct: 123 TGTFLCGREAAAAM-----IESGQGGVIVNISSLARA-GNMGQTNYAASKAGVATMAVGW 176 Query: 177 ARDLSTQGIRICTIAPGLFNTPMLAALPEKVRTFLAKSIPFPQRLGEPSEYAHLVQAIYE 236 A++L+ IR +APG+ T M AA+ + L K +P RLG+ E A V+ I E Sbjct: 177 AKELARFNIRAAAVAPGVIATEMTAAMKPEALERLEKMVPV-GRLGQAEEIASTVRFIME 235 Query: 237 NPLLNGEVIRIDGALRM 253 N +NG V IDG +R+ Sbjct: 236 NDYVNGRVFEIDGGIRL 252 Lambda K H 0.317 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 157 Number of extensions: 11 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 252 Length adjustment: 24 Effective length of query: 231 Effective length of database: 228 Effective search space: 52668 Effective search space used: 52668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory