Alignments for a candidate for pimF in Shewanella amazonensis SB2B

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Shewanella amazonensis SB2B

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate 6938034 Sama_2167 multifunctional fatty acid oxidation complex subunit alpha (RefSeq)

Query= metacyc::MONOMER-20678
         (699 letters)



>FitnessBrowser__SB2B:6938034
          Length = 706

 Score =  373 bits (957), Expect = e-107
 Identities = 253/703 (35%), Positives = 367/703 (52%), Gaps = 34/703 (4%)

Query: 11  DQVAIVTVDSP--PVNALSAAVRRGILENVNAAVADPAVQAIVLVCA-GRTFIAGADITE 67
           D +A++T+D P   +N L A     I   +    AD +++ +VL+     +F+AGADI+ 
Sbjct: 12  DGIALLTMDVPGETMNTLKAQFAPEITAILQEIKADSSIKGLVLISGKADSFVAGADISM 71

Query: 68  FG--KPPQPPAL-----NDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEAK 120
               +  +   L     + V A LE    P +AAIHG  LGGGLE+AL CH RV    +K
Sbjct: 72  LDACETAEDARLLSRQGHHVFAELEGLNIPVVAAIHGACLGGGLELALACHQRVCSDSSK 131

Query: 121 --LGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVVENLV 178
             LG+PEV+LGLLPG GGTQRLPR +G   A+ +++ G  +   +A+K GLV++VV   +
Sbjct: 132 TMLGVPEVQLGLLPGGGGTQRLPRLIGIAKALDLMLTGKQVRPKQAVKMGLVDDVVPESI 191

Query: 179 AGAVAFAKKVLAEKRPLRRLRDD--DSKLAAAKADRSI-FTNAVAAMTKKARG-LEAPFA 234
               A  +  LA K+  + L+       L      R+I F  A   + KK +G   +P  
Sbjct: 192 LLDTAI-EMALAGKKTRKPLKQPLVTKLLEGTPVGRNIMFDQATKQVLKKTQGNYPSPLK 250

Query: 235 CADAIGAAIDLPFEEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRP 294
             D +   +    E+GL+ E   F  LV + +S A R  FFA  E  K  G  D  KPR 
Sbjct: 251 IIDCVREGMAKGMEKGLEVEAAHFGALVATPESAALRSIFFATTEMKKETGAGDA-KPRK 309

Query: 295 VSRVAIIGAGTMGGGIA-MSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPP 353
           V++  ++G G MGGGIA ++   A +PV + +  ++ L   LG   K  +    R  +  
Sbjct: 310 VAKAVVLGGGLMGGGIASVTTTKAKVPVRVKDISDKGLSNALGYAYKLLDKGVKRRHMTS 369

Query: 354 DAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYL 413
               K MAL+T       VKDAD+++EAVFE + +K ++   ++       + ASNTS L
Sbjct: 370 AERDKLMALMTTTTEYRGVKDADIVVEAVFEDLNLKHQMVRDIERECGEHTIFASNTSSL 429

Query: 414 SIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVV 473
            I +IAA   RP++V+G+H+FSP   M L E++   KT+P+ + T V+ A+K  K P+VV
Sbjct: 430 PITQIAAAASRPENVIGLHYFSPVEKMPLVEVIAHEKTSPETIATTVAFARKQGKTPIVV 489

Query: 474 GVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWR- 532
             C GF  NR+LA    ++ +LL EG     +D  + KFG P+GP  + D  G+D+G + 
Sbjct: 490 KDCAGFYVNRILALYMNEAAQLLLEGQAVDHLDKALVKFGFPVGPMTLLDEVGIDVGAKI 549

Query: 533 ---SRKDRG--IKSEIA-DALCEAGRFGQKTGKGYYKY-EQGSRAPMPDPEVETLINDTL 585
                K+ G   K+  A D L    R G+K GKG+Y Y +   +    D  V +L+  T 
Sbjct: 550 SPILEKELGERFKAPAAFDKLMADDRKGRKNGKGFYLYGKAAKKGKKVDESVYSLLGLTP 609

Query: 586 AKLGLKRRDITDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPM 645
           A            EI ER V  M+NE  R LEE I A P D D+  ++G G+P + GGP 
Sbjct: 610 A------TGKEAGEIAERCVVQMLNEAVRCLEEGIIASPRDGDIGAIFGIGFPPFLGGPF 663

Query: 646 HYADSVGLKHIAERLSAYAKATNDPSLEPAPLLARLAAEGKTF 688
            Y D++G   +   L  Y     D    PA LL  +AAEGKTF
Sbjct: 664 RYMDTLGAAKMVRLLEGYQSKYGD-RFAPAALLKAMAAEGKTF 705


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1038
Number of extensions: 52
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 706
Length adjustment: 39
Effective length of query: 660
Effective length of database: 667
Effective search space:   440220
Effective search space used:   440220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources