Alignments for a candidate for putP in Shewanella amazonensis SB2B

GapMind for catabolism of small carbon sources

Alignments for a candidate for putP in Shewanella amazonensis SB2B

Align L-proline uptake porter, PutP (characterized)
to candidate 6938044 Sama_2177 sodium/proline symporter (RefSeq)

Query= TCDB::Q9I5F5
         (506 letters)



>FitnessBrowser__SB2B:6938044
          Length = 482

 Score =  512 bits (1319), Expect = e-149
 Identities = 274/499 (54%), Positives = 351/499 (70%), Gaps = 26/499 (5%)

Query: 1   MSVNTPTLITFVIYIAAMVLIGLAAYRSTNNFSDYILGGRSLGSFVTALSAGASDMSGWL 60
           MS++TP L+TFV Y+A M+ IG  AY++T++  DYILGGR +G  VTALS GASDMSGWL
Sbjct: 1   MSIDTPILVTFVGYLALMMGIGFWAYKATDSVDDYILGGRKMGPGVTALSVGASDMSGWL 60

Query: 61  LMGLPGAVYLSGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNGNALTLPDYFTNRFED 120
           L+GLPGAVYL GL E+WI IGL++GA+LNWLFVA RLR+ T+   NALTLPD+F  RF D
Sbjct: 61  LLGLPGAVYLGGLGEAWIGIGLVIGAWLNWLFVAKRLRIYTQLADNALTLPDFFEKRFHD 120

Query: 121 NSRLLRIFSALVILVFFTIYCASGIVAGARLFESTFGLSYETALWAGAAATIAYTFIGGF 180
            + LLR  SAL ILVFFT Y +SG+V GA LFE  FGL Y  AL  G+A  + YTF+GGF
Sbjct: 121 KAGLLRFVSALTILVFFTFYASSGMVGGAILFEKVFGLDYTAALVIGSAIIVGYTFVGGF 180

Query: 181 LAVSWTDTVQASLMIFALILTPVIVMLATGGVEPTFTAIELK---DATSFDML----KGA 233
            AVSWTD  Q  LM+ AL++ P+ +          FT  E     D  S +ML    +G 
Sbjct: 181 FAVSWTDFFQGCLMLIALLIVPMAI----------FTNSESHASIDTLSPEMLSLIGEGT 230

Query: 234 SFIGVISLMAWGLGYFGQPHILARFMAADSVKSIPAARRISMTWMILCLGGAVAVGFFGI 293
           + +GV SLMAWGLGYFGQPHIL+RFMA +  + +P +RRI+M+WMI+ L GA+A G  G 
Sbjct: 231 TLMGVASLMAWGLGYFGQPHILSRFMAIERPEHLPVSRRIAMSWMIVSLMGALATGLAGS 290

Query: 294 AYFQAHPEQAGAVSENPERVFIELAKILFNPWIAGVLLSAILAAVMSTLSCQLLVCSSAL 353
            YF   P        N E VFI LA++ FNPWIAG+L++AIL+A+MST+  QLLVCSS +
Sbjct: 291 LYFAKEP------LANAETVFIHLAQVAFNPWIAGLLIAAILSAIMSTIDSQLLVCSSVV 344

Query: 354 TEDFYKAFLRKGASQLELVWVGRAMVLLVAVIAIWLASNPENRVLGLVSYAWAGFGAAFG 413
           TEDFY+  LR  A   EL+ VGR  VL +A+IA  +A +P++ VLGLVSYAWAGFGAAFG
Sbjct: 345 TEDFYRK-LRPKADDNELMRVGRIGVLAIAIIAGVVALDPQSSVLGLVSYAWAGFGAAFG 403

Query: 414 PLVLFSLLWKRMTRNGALAGMIVGAATVILWKNLL-GWTGLYEIIPGFLFASVAIVVFSL 472
           P+VL SL W+  ++ GALA +I GA TV++WK L  G+  LYEI+PGF+ A++A V+ SL
Sbjct: 404 PVVLLSLFWRGYSKTGALATIITGAVTVVVWKQLSGGFFDLYEILPGFILATLAGVLISL 463

Query: 473 LGKAPSTSMLKRFDDAEQE 491
           +  A + S+   FD  + +
Sbjct: 464 VAPA-NDSVRAEFDSFQSK 481


Lambda     K      H
   0.326    0.138    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 835
Number of extensions: 41
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 506
Length of database: 482
Length adjustment: 34
Effective length of query: 472
Effective length of database: 448
Effective search space:   211456
Effective search space used:   211456
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources