Alignments for a candidate for patA in Shewanella amazonensis SB2B

GapMind for catabolism of small carbon sources

Alignments for a candidate for patA in Shewanella amazonensis SB2B

Align putrescine-pyruvate transaminase (EC 2.6.1.113) (characterized)
to candidate 6938540 Sama_2643 putative aminotransferase (RefSeq)

Query= BRENDA::Q9I6J2
         (456 letters)



>FitnessBrowser__SB2B:6938540
          Length = 460

 Score =  593 bits (1529), Expect = e-174
 Identities = 289/445 (64%), Positives = 342/445 (76%), Gaps = 2/445 (0%)

Query: 14  QALSRDHHLPPFTDYKQLNEKGARIITKAEGVYIWDSEGNKILDAMAGLWCVNVGYGREE 73
           QA+   HHL PFTD   L ++G R+I +AEGVYIWD++GNK+LDAMAGLWCVNVGYGR+ 
Sbjct: 13  QAMDAAHHLHPFTDSADLAKRGTRVIERAEGVYIWDAKGNKLLDAMAGLWCVNVGYGRKS 72

Query: 74  LVQAATRQMRELPFYNLFFQTAHPPVVELAKAIADVAPEGMNHVFFTGSGSEANDTVLRM 133
           +  AA  Q++ LPFYN FFQ  H P + LA  IA +AP  MN VFFTGSGSEANDT LRM
Sbjct: 73  IADAAYAQLQTLPFYNNFFQCTHEPAIRLASKIASLAPGHMNRVFFTGSGSEANDTNLRM 132

Query: 134 VRHYWATKGQPQKKVVIGRWNGYHGSTVAGVSLGGMKALHEQGDFPIPGIVHIAQPYWYG 193
           VR YW  KG P KK +I R N YHGSTVAG SLGGM  +H+QGD PIPGIVHI QPYW+G
Sbjct: 133 VRRYWDLKGMPSKKTIISRKNAYHGSTVAGASLGGMGFMHQQGDLPIPGIVHIDQPYWFG 192

Query: 194 EGGDMSPDEFGVWAAEQLEKKILEVGEENVAAFIAEPIQGAGGVIVPPDTYWPKIREILA 253
           EG DMSP+ FG+  A+ LE KILE+GE+ VAAFIAEP QGAGGVI+PPD+YW +I+ IL 
Sbjct: 193 EGRDMSPEAFGIKTAQALEAKILELGEDKVAAFIAEPFQGAGGVIIPPDSYWNEIKRILE 252

Query: 254 KYDILFIADEVICGFGRTGEWFGSQYYGNAPDLMPIAKGLTSGYIPMGGVVVRDEIVEVL 313
           KY+ILFI DEVI GFGRTG WF +Q  G  PDL+ IAKG+TSGYIPMGGV+V D + +VL
Sbjct: 253 KYNILFILDEVISGFGRTGNWFAAQTLGLKPDLITIAKGMTSGYIPMGGVIVSDRVADVL 312

Query: 314 -NQGGEFYHGFTYSGHPVAAAVALENIRILREEKIIEKVKAETAPYLQKRWQELADHPLV 372
            + GGEF HGFTYSGHPVAAAVALENIRIL EE++++KV+ +T PYLQ R Q L+ HPLV
Sbjct: 313 ISDGGEFAHGFTYSGHPVAAAVALENIRILEEERLVDKVRTDTGPYLQDRLQTLSAHPLV 372

Query: 373 GEARGVGMVAALELVKNKKTRERF-TDKGVGMLCREHCFRNGLIMRAVGDTMIISPPLVI 431
           GE RG+GMV A+ELV +K +  RF ++   GMLCRE C  +GL+MRAVGDTMIISPPL I
Sbjct: 373 GEVRGMGMVGAIELVADKHSMVRFGSEISAGMLCREACIESGLVMRAVGDTMIISPPLCI 432

Query: 432 DPSQIDELITLARKCLDQTAAAVLA 456
              +IDELI  A + L  T   + A
Sbjct: 433 TRDEIDELIFKASQALSLTLEKIAA 457


Lambda     K      H
   0.320    0.138    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 692
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 456
Length of database: 460
Length adjustment: 33
Effective length of query: 423
Effective length of database: 427
Effective search space:   180621
Effective search space used:   180621
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources