Align Putrescine transporter PotE; Putrescine-proton symporter / putrescine-ornithine antiporter (characterized)
to candidate 6939241 Sama_3335 putrescine transporter (RefSeq)
Query= SwissProt::P0AAF1 (439 letters) >FitnessBrowser__SB2B:6939241 Length = 438 Score = 665 bits (1717), Expect = 0.0 Identities = 327/430 (76%), Positives = 376/430 (87%) Query: 6 SNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIISWLVTAVGSMALAWAFAKCGMF 65 +NK+GVVQLTILT+VNMMGSGIIMLPT+LA+VGTISI+SWLVTA GS ALA+AFAKCGMF Sbjct: 5 NNKIGVVQLTILTIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAFAFAKCGMF 64 Query: 66 SRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELLGASLSPVQIG 125 S+KSGGMGGYAEYAFG+SGNFMANYTY VSLLIANVAIAISAVGYG L LSP+ Sbjct: 65 SKKSGGMGGYAEYAFGRSGNFMANYTYAVSLLIANVAIAISAVGYGAVLFDVELSPIATC 124 Query: 126 LATIGVLWICTVANFGGARITGQISSITVWGVIIPVVGLCIIGWFWFSPTLYVDSWNPHH 185 LATIGVLW+ TVANFGGARITGQ+SSITVWG+I+PV+G+ +IGWFWF LY +WNPH Sbjct: 125 LATIGVLWLATVANFGGARITGQVSSITVWGIILPVIGVSLIGWFWFDFDLYKSAWNPHD 184 Query: 186 APFFSAVGSSIAMTLWAFLGLESACANTDVVENPERNVPIAVLGGTLGAAVIYIVSTNVI 245 PFF A+G SIAMTLWAFLGLESACAN++ VENPE+NVPIAV+GGTLGAA+IYIVSTNVI Sbjct: 185 LPFFEALGGSIAMTLWAFLGLESACANSEAVENPEKNVPIAVMGGTLGAALIYIVSTNVI 244 Query: 246 AGIVPNMELANSTAPFGLAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSD 305 AGIVPN ELA S APFGLAFAQMF P +G ++MA ++SC GSLLGWQFTIAQVFK+S+D Sbjct: 245 AGIVPNGELALSNAPFGLAFAQMFNPTIGAIVMACAIISCTGSLLGWQFTIAQVFKASAD 304 Query: 306 EGYFPKIFSRVTKVDAPVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYI 365 EG+FPK+FS+VTK DAPV GML IV IQS L+LMTISPSLN QF LVNLAVVTNIIPYI Sbjct: 305 EGFFPKLFSKVTKADAPVLGMLAIVSIQSVLSLMTISPSLNKQFEALVNLAVVTNIIPYI 364 Query: 366 LSMAALVIIQKVANVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLY 425 LSMAAL ++QK +P SKA++AN +A +GA+YSFYALYSSGE A++ GSI TF GWTLY Sbjct: 365 LSMAALGVMQKQMKIPVSKARIANCMAVMGALYSFYALYSSGETAVMLGSIATFFGWTLY 424 Query: 426 GLVSPRFELK 435 G++S + EL+ Sbjct: 425 GVISKKNELR 434 Lambda K H 0.323 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 829 Number of extensions: 28 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 439 Length of database: 438 Length adjustment: 32 Effective length of query: 407 Effective length of database: 406 Effective search space: 165242 Effective search space used: 165242 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory