GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Shewanella amazonensis SB2B

Align lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)
to candidate 6938534 Sama_2637 succinate-semialdehyde dehydrogenase (NAD(P)(+)) (RefSeq)

Query= BRENDA::P25553
         (479 letters)



>lcl|FitnessBrowser__SB2B:6938534 Sama_2637 succinate-semialdehyde
           dehydrogenase (NAD(P)(+)) (RefSeq)
          Length = 480

 Score =  334 bits (857), Expect = 3e-96
 Identities = 177/468 (37%), Positives = 274/468 (58%), Gaps = 3/468 (0%)

Query: 10  YIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPAIERA 69
           YI+G++        + + NPAT   I+ +P    ++ R+AI AAE A P W AL A ER 
Sbjct: 14  YINGEWRDALSGETVTIANPATNEAIASVPVMGRDETREAIAAAEAALPAWRALTAKERG 73

Query: 70  SWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGEIIQS 129
           + LR+    + E A +++ ++  E GK    A+ EV + A +I++ AE A+R  G+ I  
Sbjct: 74  AKLRRWYELMLENADDLALMMTTEQGKPLAEAKGEVVYAASFIEWFAEEAKRLYGDTIPG 133

Query: 130 DRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNAIAFA 189
            +  + I++ K+ +GVT  I PWNFP  +I RK  PAL  G T+++KP+  TP  A+A A
Sbjct: 134 HQGDKRIMVIKQGVGVTAAITPWNFPAAMITRKAGPALAAGCTMIVKPAPQTPFTALALA 193

Query: 190 KIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNITKVC 249
           ++  E G+P GVF++V G    +G EL  NP V  +S TGS   G K+M   A  + KV 
Sbjct: 194 ELAAEAGIPPGVFSVVTGDAVAIGNELCENPVVRKLSFTGSTGVGIKLMQQCAPTLKKVS 253

Query: 250 LELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRLGEAM 309
           LELGG AP IV +DADL+ AV+  + S+  N+GQ C CA R+YVQ G+YD F  +L  A+
Sbjct: 254 LELGGNAPFIVFNDADLDAAVEGAMISKYRNAGQTCVCANRLYVQDGVYDAFAQKLAAAV 313

Query: 310 QAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEGKGYYYPPTL 369
             ++ GN AE   +  GPLINAAALE+V+  +  A+++GA +  GGK + G   +  P +
Sbjct: 314 AKLKVGNGAEPG-VTTGPLINAAALEKVQSHLQDALDKGATLVAGGKPLGGN--FMEPAI 370

Query: 370 LLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKG 429
           + +V   M +  EETFGP+ P+  F  ++D I  AND+++GL +  Y +++++  K  + 
Sbjct: 371 VTNVDASMKVAREETFGPLAPLFRFSDVDDVIRQANDTEFGLAAYFYGRDISLIWKVAEA 430

Query: 430 LKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYL 477
           L++G   +N            G + SG+G    K+G+ EY++ + + L
Sbjct: 431 LEYGMVGVNTGLISTEVAPFGGMKSSGLGREGSKYGIDEYVEIKYICL 478


Lambda     K      H
   0.318    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 553
Number of extensions: 24
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 479
Length of database: 480
Length adjustment: 34
Effective length of query: 445
Effective length of database: 446
Effective search space:   198470
Effective search space used:   198470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory