Align PTS system, fructose-specific, IIA component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized)
to candidate 6938992 Sama_3090 nitrogen regulatory IIA protein (RefSeq)
Query= TCDB::Q3JZE3 (149 letters) >FitnessBrowser__SB2B:6938992 Length = 147 Score = 60.5 bits (145), Expect = 1e-14 Identities = 39/123 (31%), Positives = 64/123 (52%), Gaps = 7/123 (5%) Query: 27 ELVSLL---KNEGFVSDEGGFIKDIYLRESEGQTGIGNYIAIPHGKSSHVTEPAVVIGIN 83 EL+S L +N S E + + RE G TGIGN IAIPHG+ + +P V+ Sbjct: 25 ELISDLAAAQNPSLSSQE--IFESLLAREKMGSTGIGNGIAIPHGRLGTIDKPLAVLIKC 82 Query: 84 SSEIPWETLDDNGVKVIVLFAVGNDSIAAQEHLKILSLFARKLGNDQIVEKLINAKNEDD 143 I ++ +D V ++ V +D Q+HL L+ A KL + QI+++L + +E + Sbjct: 83 EEAIGYDAIDKQPVDILFALLVPSDQ--CQQHLSTLAAMAEKLNDKQILKQLRKSHDEKE 140 Query: 144 VIE 146 + + Sbjct: 141 LYQ 143 Lambda K H 0.314 0.134 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 59 Number of extensions: 4 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 149 Length of database: 147 Length adjustment: 16 Effective length of query: 133 Effective length of database: 131 Effective search space: 17423 Effective search space used: 17423 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 42 (20.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory