Align L-serine ammonia-lyase (EC 4.3.1.17) (characterized)
to candidate 6937155 Sama_1325 L-serine ammonia-lyase (RefSeq)
Query= BRENDA::P16095 (454 letters) >FitnessBrowser__SB2B:6937155 Length = 458 Score = 516 bits (1328), Expect = e-151 Identities = 257/456 (56%), Positives = 333/456 (73%), Gaps = 6/456 (1%) Query: 1 MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH 60 MIS+FD+FK+GIGPSSSHTVGPMKAGK+FV + GLL V + +++GSL TGKGH Sbjct: 1 MISVFDIFKIGIGPSSSHTVGPMKAGKEFVARVESMGLLAQVDELRAELFGSLGQTGKGH 60 Query: 61 HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGN- 119 T A+I+GL G +P TVD D+I G + V +E L LAQG+ V F R++G+ +H Sbjct: 61 GTGKAVILGLMGEDPETVDTDAIDGILAQVSSQETLTLAQGK-TVKFTREDGIIYHRRKT 119 Query: 120 LPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQDAANEVS----VPYPFKSAT 175 LP H N M ++A+ G E +Y +TYYS+GGGF++DE+ + + + PY F SA Sbjct: 120 LPAHANAMTLYAFAGGECLYQRTYYSVGGGFVLDEDQIERKNPSPATPIKAAPYDFNSAQ 179 Query: 176 ELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLR 235 +LL C + G S+S L MQNEL L S+ ++ H+WQTM+ CI++G EG+LPG L+ Sbjct: 180 QLLEMCTDNGLSISSLMMQNELTLASEDDVKARLWHIWQTMKTCIEKGYKKEGMLPGGLK 239 Query: 236 VPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPA 295 + RRA L R L + + + DP+N +DWV++FAL+VNE+NAAG RVVTAPTNGA GI+PA Sbjct: 240 LRRRAPGLYRRLQAEGRNNVDPLNAMDWVDLFALSVNEQNAAGDRVVTAPTNGAAGIIPA 299 Query: 296 VLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLA 355 VL Y+D F++ V DI RY + A AIG LYK NASISGAEVGCQGEVGVACSMAAA L Sbjct: 300 VLCYFDTFVQQVDVDICARYLLTAAAIGILYKKNASISGAEVGCQGEVGVACSMAAAALT 359 Query: 356 ELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRTS 415 E++GG+ E V AAEIGMEHNLGLTCDPV G VQVPCIERNA+ +VKAINA+R+ALR Sbjct: 360 EIMGGTVEHVENAAEIGMEHNLGLTCDPVGGLVQVPCIERNAMGAVKAINASRLALRGDG 419 Query: 416 APRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKV 451 +VSLDKVI+TM +TG+DM +KY+ET++GGLA+ + Sbjct: 420 NHKVSLDKVIKTMMDTGRDMRSKYKETAKGGLAVNI 455 Lambda K H 0.318 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 628 Number of extensions: 24 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 454 Length of database: 458 Length adjustment: 33 Effective length of query: 421 Effective length of database: 425 Effective search space: 178925 Effective search space used: 178925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate 6937155 Sama_1325 (L-serine ammonia-lyase (RefSeq))
to HMM TIGR00720 (L-serine ammonia-lyase (EC 4.3.1.17))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00720.hmm # target sequence database: /tmp/gapView.19342.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00720 [M=450] Accession: TIGR00720 Description: sda_mono: L-serine ammonia-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.1e-217 709.3 0.6 1.2e-217 709.1 0.6 1.0 1 lcl|FitnessBrowser__SB2B:6937155 Sama_1325 L-serine ammonia-lyase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6937155 Sama_1325 L-serine ammonia-lyase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 709.1 0.6 1.2e-217 1.2e-217 1 450 [] 2 455 .. 2 455 .. 0.99 Alignments for each domain: == domain 1 score: 709.1 bits; conditional E-value: 1.2e-217 TIGR00720 1 isvfdlfkiGiGPssshtvGPmkaakefveelkkkgkleqvkrvkvdlyGslaltGkGhktdkavllGleGelpeev 77 isvfd+fkiGiGPssshtvGPmka+kefv++++++g l+qv++++ +l+Gsl+ tGkGh+t kav+lGl+Ge+pe+v lcl|FitnessBrowser__SB2B:6937155 2 ISVFDIFKIGIGPSSSHTVGPMKAGKEFVARVESMGLLAQVDELRAELFGSLGQTGKGHGTGKAVILGLMGEDPETV 78 79*************************************************************************** PP TIGR00720 78 diesieklleeveeekklklanqkeikfdlekdlafk.devlplhenglrlkaydeegevlkektyysvGGGfivde 153 d++ i+ +l++v ++++l+la+ k++kf++e+ ++++ +++lp h+n+++l a+++ e l+++tyysvGGGf++de lcl|FitnessBrowser__SB2B:6937155 79 DTDAIDGILAQVSSQETLTLAQGKTVKFTREDGIIYHrRKTLPAHANAMTLYAFAGG-ECLYQRTYYSVGGGFVLDE 154 *************************************999**************998.9****************** PP TIGR00720 154 eelkkeeeeee....evpypfksaaellelCkeeglsisevvlenekalrseeevraklleiwkvmeecierglkae 226 +++++++ + + +py+f+sa++lle+C+++glsis+++++ne +l+se++v+a+l +iw++m++cie+g k+e lcl|FitnessBrowser__SB2B:6937155 155 DQIERKNPSPAtpikAAPYDFNSAQQLLEMCTDNGLSISSLMMQNELTLASEDDVKARLWHIWQTMKTCIEKGYKKE 231 ****9998877677789************************************************************ PP TIGR00720 227 gvlpGglkvkrraaslkrklkakeetskdplavldwvnlyalavneenaaGgrvvtaPtnGaagiiPavlayykkfv 303 g+lpGglk++rra+ l+r+l+a+ +++ dpl+++dwv l+al+vne+naaG+rvvtaPtnGaagiiPavl y+++fv lcl|FitnessBrowser__SB2B:6937155 232 GMLPGGLKLRRRAPGLYRRLQAEGRNNVDPLNAMDWVDLFALSVNEQNAAGDRVVTAPTNGAAGIIPAVLCYFDTFV 308 ***************************************************************************** PP TIGR00720 304 eeaseekvvrflltagaiGilykenasisgaevGCqgevGvacsmaaaglaellggtpeqvenaaeiamehnlGltC 380 +++ ++ r+llta+aiGilyk+nasisgaevGCqgevGvacsmaaa+l+e++ggt+e venaaei+mehnlGltC lcl|FitnessBrowser__SB2B:6937155 309 QQVDVDICARYLLTAAAIGILYKKNASISGAEVGCQGEVGVACSMAAAALTEIMGGTVEHVENAAEIGMEHNLGLTC 385 ***************************************************************************** PP TIGR00720 381 dPvgGlvqiPCiernaiaavkainaarlalkedgkkkvsldkvietmretGkdmkakyketskgGlavkv 450 dPvgGlvq+PCierna++avkaina+rlal++dg++kvsldkvi+tm++tG+dm++kyket+kgGlav++ lcl|FitnessBrowser__SB2B:6937155 386 DPVGGLVQVPCIERNAMGAVKAINASRLALRGDGNHKVSLDKVIKTMMDTGRDMRSKYKETAKGGLAVNI 455 ********************************************************************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (450 nodes) Target sequences: 1 (458 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 9.29 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory