GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aglK' in Shewanella amazonensis SB2B

Align Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale)
to candidate 6937298 Sama_1468 putative macrolide efflux ABC transporter, ATP-binding protein (RefSeq)

Query= uniprot:A8LLL2
         (373 letters)



>FitnessBrowser__SB2B:6937298
          Length = 230

 Score =  117 bits (292), Expect = 4e-31
 Identities = 75/209 (35%), Positives = 117/209 (55%), Gaps = 14/209 (6%)

Query: 15  DVKVLSNINLDIQQGELIVFVGPSGCGKSTLLRMIAGLEKITGGTLEIDGTVVNDVPP-- 72
           +V+ L  +++ I+Q E +  +GPSG GKSTL+ +I  L++ T G   ++G+ V  +    
Sbjct: 17  EVQALRGVDIHIRQNEFVAIMGPSGSGKSTLMNIIGCLDRPTSGNYLLNGSAVGGLSDDA 76

Query: 73  ----AQRGIAMVFQSYALYPHMTVRENMSFALKIAKKSQAEIDAAVEAAAEKLQLGQYLD 128
                 R I  VFQS+ L P ++  +N+   L+ ++  + +   A+E   E++ LGQ LD
Sbjct: 77  LSAVRNREIGFVFQSFHLLPRLSALDNVLLPLRFSETPRGDRQHAIELL-ERVGLGQRLD 135

Query: 129 RLPKALSGGQRQRVAIGRSIVRDPKVYLFDEPLSNLDAALRVATRLEIAQLKEAMPES-- 186
             P  LSGGQRQRVAI R++V  P + L DEP   LD+     T +EI  L + +  S  
Sbjct: 136 HRPNQLSGGQRQRVAIARALVNRPTLLLADEPTGALDS----KTSVEIMALFDELHLSGQ 191

Query: 187 TMVYVTHDQVEAMTLATRIVVLAGGGIAQ 215
           T+V VTH++ E    A RI+ +  G + Q
Sbjct: 192 TIVLVTHEE-EVAECAGRIIRMRDGVVQQ 219


Lambda     K      H
   0.316    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 191
Number of extensions: 19
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 373
Length of database: 230
Length adjustment: 26
Effective length of query: 347
Effective length of database: 204
Effective search space:    70788
Effective search space used:    70788
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory