Align Methylmalonate-semialdehyde dehydrogenase [acylating]; MMSDH; EC 1.2.1.27 (characterized)
to candidate 6937206 Sama_1376 methylmalonate-semialdehyde dehydrogenase (RefSeq)
Query= SwissProt::P28810 (497 letters) >FitnessBrowser__SB2B:6937206 Length = 497 Score = 752 bits (1942), Expect = 0.0 Identities = 363/497 (73%), Positives = 412/497 (82%) Query: 1 MSVPVRHLIAGAFVEGLGAQRIPVSNPLDNSTLAEIACASAEQVEQAVASARETFASWKE 60 M V+H I G F G G + I V+NP +N +AE+ CAS E+V A+ASA+ F SWKE Sbjct: 1 MITQVKHYIDGEFTTGQGERIITVTNPANNQPIAEVRCASDEEVHGAIASAKAAFQSWKE 60 Query: 61 TPVSERARVMLRYQALLKEHHDELAKIVSSELGKTFEDAKGDVWRGIEVVEHACNVPSLL 120 PVSERARVMLRYQALLKEHHDELA I++ E GKTFEDAKGDVWRGIEV EHACN+ SLL Sbjct: 61 VPVSERARVMLRYQALLKEHHDELATILAKETGKTFEDAKGDVWRGIEVAEHACNIASLL 120 Query: 121 MGETVENVARNIDTYSITQPLGVCVGITPFNFPAMIPLWMFPLAIACGNAFILKPSEQVP 180 MGETVENVAR IDTYS TQPLGVC GITPFNFPAMIPLWMFPLA+ACGN F+LKPSEQ P Sbjct: 121 MGETVENVARKIDTYSYTQPLGVCAGITPFNFPAMIPLWMFPLAVACGNTFVLKPSEQDP 180 Query: 181 LTSVRLAELFLEAGAPKGVLQVVHGGKEQVDQLLKHPQVKAVSFVGSVAVGQYVYHTGTA 240 +T +RLAELF +AGAPKGVLQ+VHG K VD LL HP +KA+SFVGSV VGQYVY TGT Sbjct: 181 MTPLRLAELFEQAGAPKGVLQLVHGDKSAVDILLTHPDIKAISFVGSVGVGQYVYKTGTD 240 Query: 241 HNKRVQSFAGAKNHMVIMPDADKAQVISNLVGASVGAAGQRCMAISVAVLVGAAREWIPE 300 + KRVQ+FAGAKNH VIMPDA+K QVI+NLVGASVGAAGQRCMAISVAV VGAA+EWIPE Sbjct: 241 NLKRVQAFAGAKNHCVIMPDANKQQVINNLVGASVGAAGQRCMAISVAVFVGAAKEWIPE 300 Query: 301 IRDALAKVRPGPWDDSGASYGPVINPQAKARIERLIGQGVEEGAQLLLDGRGYKVEGYPD 360 +++ALAKVRPG WDD A YGP+I+P AKAR+ +LI QG EGA+ LLDG + V GY Sbjct: 301 LKEALAKVRPGLWDDKDAGYGPLISPAAKARVLKLIEQGKAEGAECLLDGSDFTVPGYES 360 Query: 361 GNWVGPTLFAGVRPDMAIYREEVFGPVLCLAEVDSLEQAIRLINESPYGNGTSIFTSSGA 420 GNWVGPT+F V DM+IY+EE+FGPVLC EVD LE AI L+N+SPYGNGTSIFT+SGA Sbjct: 361 GNWVGPTMFDKVTTDMSIYKEEIFGPVLCCMEVDELEDAIELVNKSPYGNGTSIFTASGA 420 Query: 421 AARTFQHHIEVGQVGINIPIPVPLPFFSFTGWKGSFYGDLHAYGKQGVRFYTETKTVTAR 480 AAR +QH IEVGQVGIN+PIPVPLPFFSFTGWKGSFYGD HAYGKQ +RF+TETKT+T R Sbjct: 421 AARKYQHEIEVGQVGINVPIPVPLPFFSFTGWKGSFYGDQHAYGKQAIRFFTETKTITCR 480 Query: 481 WFDSDSVAGTNFSIQMR 497 WF+ D G N +I +R Sbjct: 481 WFEDDIPHGPNMTIALR 497 Lambda K H 0.319 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 864 Number of extensions: 35 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 497 Length of database: 497 Length adjustment: 34 Effective length of query: 463 Effective length of database: 463 Effective search space: 214369 Effective search space used: 214369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate 6937206 Sama_1376 (methylmalonate-semialdehyde dehydrogenase (RefSeq))
to HMM TIGR01722 (mmsA: methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01722.hmm # target sequence database: /tmp/gapView.21017.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01722 [M=477] Accession: TIGR01722 Description: MMSDH: methylmalonate-semialdehyde dehydrogenase (acylating) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.9e-253 826.7 0.2 3.2e-253 826.5 0.2 1.0 1 lcl|FitnessBrowser__SB2B:6937206 Sama_1376 methylmalonate-semiald Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6937206 Sama_1376 methylmalonate-semialdehyde dehydrogenase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 826.5 0.2 3.2e-253 3.2e-253 1 477 [] 5 481 .. 5 481 .. 1.00 Alignments for each domain: == domain 1 score: 826.5 bits; conditional E-value: 3.2e-253 TIGR01722 1 vkhlidGkfvegksdkyipvsnpatnevlakvaeasaeevdaavasaretfaawaetsvaerarvllryqallkehr 77 vkh+idG+f g++++ i v+npa+n+ +a+v +as eev+ a+asa+ +f++w+e +v+erarv+lryqallkeh+ lcl|FitnessBrowser__SB2B:6937206 5 VKHYIDGEFTTGQGERIITVTNPANNQPIAEVRCASDEEVHGAIASAKAAFQSWKEVPVSERARVMLRYQALLKEHH 81 89*************************************************************************** PP TIGR01722 78 deiaklisaeqGktledakGdvarGlevvehacsvtslllGetvesvakdvdvysirqplGvvaGitpfnfpamipl 154 de+a +++ e Gkt+edakGdv+rG+ev ehac+++sll+Getve+va+++d+ys++qplGv+aGitpfnfpamipl lcl|FitnessBrowser__SB2B:6937206 82 DELATILAKETGKTFEDAKGDVWRGIEVAEHACNIASLLMGETVENVARKIDTYSYTQPLGVCAGITPFNFPAMIPL 158 ***************************************************************************** PP TIGR01722 155 wmfplaiacGntfvlkpsekvpsaavklaellseaGapdGvlnvvhGdkeavdrllehpdvkavsfvGsvavgeyiy 231 wmfpla+acGntfvlkpse++p + ++lael+++aGap+Gvl++vhGdk avd ll hpd+ka+sfvGsv vg+y+y lcl|FitnessBrowser__SB2B:6937206 159 WMFPLAVACGNTFVLKPSEQDPMTPLRLAELFEQAGAPKGVLQLVHGDKSAVDILLTHPDIKAISFVGSVGVGQYVY 235 ***************************************************************************** PP TIGR01722 232 etgsahgkrvqalaGaknhmvvlpdadkeaaldalvgaavGaaGqrcmaisaavlvGaakelveeireraekvrvga 308 +tg+ + krvqa+aGaknh v++pda+k++++++lvga+vGaaGqrcmais+av vGaake+++e++e+++kvr+g lcl|FitnessBrowser__SB2B:6937206 236 KTGTDNLKRVQAFAGAKNHCVIMPDANKQQVINNLVGASVGAAGQRCMAISVAVFVGAAKEWIPELKEALAKVRPGL 312 ***************************************************************************** PP TIGR01722 309 gddpgaelGplitkqakervasliasgakeGaevlldGrgykveGyeeGnfvGitllervkpdmkiykeeifGpvlv 385 +dd a +Gpli+++ak+rv +li++g +eGae lldG ++v Gye Gn+vG+t++++v +dm+iykeeifGpvl+ lcl|FitnessBrowser__SB2B:6937206 313 WDDKDAGYGPLISPAAKARVLKLIEQGKAEGAECLLDGSDFTVPGYESGNWVGPTMFDKVTTDMSIYKEEIFGPVLC 389 ***************************************************************************** PP TIGR01722 386 vleadtleeaiklinespyGnGtaiftsdGaaarkfqheievGqvGvnvpipvplpffsftGwkdslfGdlhiyGkq 462 +e+d le+ai+l+n+spyGnGt+ift++Gaaark+qheievGqvG+nvpipvplpffsftGwk+s++Gd+h+yGkq lcl|FitnessBrowser__SB2B:6937206 390 CMEVDELEDAIELVNKSPYGNGTSIFTASGAAARKYQHEIEVGQVGINVPIPVPLPFFSFTGWKGSFYGDQHAYGKQ 466 ***************************************************************************** PP TIGR01722 463 Gvrfytrlktvtarw 477 +rf+t++kt+t+rw lcl|FitnessBrowser__SB2B:6937206 467 AIRFFTETKTITCRW 481 *************** PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (477 nodes) Target sequences: 1 (497 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 10.86 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory