GapMind for catabolism of small carbon sources

 

Alignments for a candidate for prpC in Shewanella amazonensis SB2B

Align 2-methylcitrate synthase (EC 2.3.3.5) (characterized)
to candidate 6937252 Sama_1422 type II citrate synthase (RefSeq)

Query= reanno::pseudo6_N2E2:Pf6N2E2_6062
         (375 letters)



>FitnessBrowser__SB2B:6937252
          Length = 428

 Score =  191 bits (484), Expect = 4e-53
 Identities = 129/389 (33%), Positives = 196/389 (50%), Gaps = 30/389 (7%)

Query: 11  GLRGQVAGQTALSTVGQSGAGLTYRGYDVRDLAADAQFEEVAYLLLYGELPTQAQLDAYT 70
           G     + ++A++ +      L +RGY +  LA ++ + E+ Y LLYGELPT+AQ + + 
Sbjct: 46  GFLATASCESAITYIDGDKGILLHRGYPIEQLAVESNYLELCYALLYGELPTKAQYEQFA 105

Query: 71  GKLRQLRDLPQALKEVLERIPADAHPMDVMRTGCSFLGNLEP-EQDFSQQHDKTDRLLAA 129
             ++    + + L         DAHPM ++   C   G L    QD    +D   R +AA
Sbjct: 106 HTVKSHTMVHEQLAFFFRGFRRDAHPMAML---CGVTGALSAFYQDSLDVNDARHREIAA 162

Query: 130 FP--------AIMCYWYRFSHQGQRIECVTDEVSIGGHFLHLLHGKKPSELHV-----KV 176
           +         A MCY Y     GQ      +++S  G+FL ++      E  V     + 
Sbjct: 163 YRLVSKMPTLAAMCYKYSI---GQPFVYPRNDLSYAGNFLSMMFAVPCEEYKVNPIIERA 219

Query: 177 MNVSLILYAEHEFNASTFTARVCASTLSDLFSCITAAIGSLRGPLHGGANEAAMEMIERF 236
           M+   IL+A+HE NAST T R+  S+ ++ F+CI A I SL GP HGGANEA + M+E  
Sbjct: 220 MDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIASLWGPAHGGANEACLRMLEEI 279

Query: 237 SSPQEAIEGTLGMLARKD--KIMGFGHAIYKDNDPRNEVIKGWSKKLADE--VGDTVLFP 292
            +     E       + D  ++MGFGH +YK+ DPR +V++    ++  E  V D +L  
Sbjct: 280 GTVDRIPEFIDRAKDKNDPFRLMGFGHRVYKNFDPRAKVMRETCHEVLSELKVNDPLLDV 339

Query: 293 VSE----AIDKTMWEQKKLFPNADFYHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVFE- 347
             E    A++   +  KKL+PN DFY       +GIPT +FT IF  +R  GW +H  E 
Sbjct: 340 AMELERIALNDEYFISKKLYPNVDFYSGIIMKAIGIPTSMFTVIFALARTVGWISHWKEM 399

Query: 348 -QRANNRIIRPSAEYTGVEQRKFVPIEQR 375
             +  ++I RP   YTG   R FV +++R
Sbjct: 400 LDQPGHKISRPRQLYTGEHSRDFVSVDKR 428


Lambda     K      H
   0.321    0.135    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 368
Number of extensions: 23
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 428
Length adjustment: 31
Effective length of query: 344
Effective length of database: 397
Effective search space:   136568
Effective search space used:   136568
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory