GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Dshi_0546 in Shewanella amazonensis SB2B

Align ABC transporter for Xylitol, ATPase component (characterized)
to candidate 6939439 Sama_3531 molybdenum ABC transporter, ATP-binding protein (RefSeq)

Query= reanno::Dino:3607124
         (338 letters)



>lcl|FitnessBrowser__SB2B:6939439 Sama_3531 molybdenum ABC
           transporter, ATP-binding protein (RefSeq)
          Length = 370

 Score =  141 bits (356), Expect = 2e-38
 Identities = 83/228 (36%), Positives = 128/228 (56%), Gaps = 10/228 (4%)

Query: 29  GEFVVFVGPSGCGKSTLLRTLAGLEGVSSGRIEIGGR------DVTTVEPADRDLAMVFQ 82
           GEF+  VGPSG GK+TLLR +AGL    +G I  G R      +     P +R +  V Q
Sbjct: 28  GEFLAVVGPSGGGKTTLLRMIAGLAKPENGSIRCGKRIWFDSDEGIHCSPQNRHIGFVPQ 87

Query: 83  SYALYPHMTVRENMEFGMKVNGFEPDLRKERIAEAARVLQLEDYLDRKPGQLSGGQRQRV 142
            + L+P ++   N+   +  +      R+ R   A   + L    DR P QLSGGQ+QRV
Sbjct: 88  HFGLFPKLSALGNIMAAL--DHLPSQERRPRALAALEKVNLHGLTDRLPSQLSGGQKQRV 145

Query: 143 AIGRAIVKNPSVFLFDEPLSNLDAKLRVQMRVELEGLHKQLGATMIYVTHDQVEAMTMAD 202
           A+ RA+ + P V L DEP S +D + R ++ +EL  L  +L   +I VTHD  EA+ +AD
Sbjct: 146 ALARALAREPRVLLLDEPFSAVDRETRERLYLELARLKAELNIPVIMVTHDLNEALLLAD 205

Query: 203 KIVVLNRGRIEQVGSPMDLYHKPNSRFVAEFIGSPAMNVFSSDVGLQD 250
            ++++++G + Q G+P D++ +P +  VA  +G    N+F++ V  QD
Sbjct: 206 SMLLISQGHMLQHGTPQDVFSRPRNEAVARQMG--LRNIFNAHVVAQD 251


Lambda     K      H
   0.320    0.139    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 261
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 338
Length of database: 370
Length adjustment: 29
Effective length of query: 309
Effective length of database: 341
Effective search space:   105369
Effective search space used:   105369
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory