GapMind for catabolism of small carbon sources


Protein SMc02037 in Sinorhizobium meliloti 1021

Annotation: FitnessBrowser__Smeli:SMc02037

Length: 256 amino acids

Source: Smeli in FitnessBrowser

Candidate for 7 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-sorbitol (glucitol) catabolism sdh hi L-iditol 2-dehydrogenase (EC (characterized) 50% 95% 243.4 D-arabinitol 4-dehydrogenase (EC; D-arabinitol dehydrogenase (NADP+) (EC 45% 215.3
D-mannitol catabolism mt2d lo NADP-dependent mannitol dehydrogenase; MtDH; Mannitol 2-dehydrogenase [NADP(+)]; Allergen Alt a 8; EC (characterized) 39% 95% 173.7 L-iditol 2-dehydrogenase (EC 50% 243.4
L-rhamnose catabolism LRA1 lo L-rhamnose-1-dehydrogenase; EC (characterized) 35% 98% 159.8 L-iditol 2-dehydrogenase (EC 50% 243.4
L-fucose catabolism fucDH lo Short-chain alcohol dehydrogenase protein (characterized, see rationale) 36% 100% 145.6 L-iditol 2-dehydrogenase (EC 50% 243.4
D-xylose catabolism DKDP-dehydrog lo SDR family oxidoreductase (characterized, see rationale) 36% 100% 144.1 L-iditol 2-dehydrogenase (EC 50% 243.4
2-deoxy-D-ribonate catabolism deoxyribonate-dehyd lo 2-deoxy-D-ribonate 3-dehydrogenase (characterized) 33% 94% 125.6 L-iditol 2-dehydrogenase (EC 50% 243.4
2-deoxy-D-ribose catabolism deoxyribonate-dehyd lo 2-deoxy-D-ribonate 3-dehydrogenase (characterized) 33% 94% 125.6 L-iditol 2-dehydrogenase (EC 50% 243.4

Sequence Analysis Tools

View SMc02037 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory