Align alpha-ketoglutarate TRAP transporter, 4TM/12TM components (characterized)
to candidate SM_b20291 SM_b20291 hypothetical protein
Query= reanno::psRCH2:GFF84 (674 letters) >FitnessBrowser__Smeli:SM_b20291 Length = 827 Score = 364 bits (935), Expect = e-105 Identities = 225/654 (34%), Positives = 345/654 (52%), Gaps = 80/654 (12%) Query: 44 RAGHVGFLLLLVFLCYPA-RGNGKPFQPVA-WLLGLAGFATFFYQWYFEADLIQRSGDMT 101 RA H+GF L L FL YPA + + + P+A W+LG G Y + F +L R G + Sbjct: 29 RAIHLGFALFLTFLAYPALKSSPRDRVPLADWVLGALGAFAGAYLFLFYGELAGRPGQPS 88 Query: 102 TADMVVGLTLIVLVFEAARRVMGIALPIICALFLAYGLLGEYLPGDLAHRGYYLDQIVNQ 161 T D+V G I+L+ EA RR +G+ + + +F+ Y G+Y+P + HRG L + +N Sbjct: 89 TLDLVTGTAGILLLLEATRRALGLPMVFVAGVFIFYTFAGQYMPDVIQHRGASLVKFINH 148 Query: 162 LSFGTEGLYGTPTYVSATYIFLFILFGSFLEQAGMIKLFTDFAMGLFGHKLGGPAKVSVV 221 TEG++G VS +++FLF+LFG+ LE+AG ++ L GH GGPAKV+VV Sbjct: 149 QWLTTEGVFGIALGVSTSFVFLFVLFGTLLEKAGAGNWMMQISIALLGHLRGGPAKVAVV 208 Query: 222 SSALMGTITGSGVANVVTTGQFTIPLMKRFGYRPAFAGGVEATSSMGSQIMPPVMGAVAF 281 SSAL G ++GS V+NVV+ G FTIPLMKR G AG +EA++S+ QIMPPVMGA AF Sbjct: 209 SSALNGVVSGSSVSNVVSGGIFTIPLMKRTGLSGVKAGAIEASASINGQIMPPVMGAAAF 268 Query: 282 IMAETINVPFVEIAKAALIPALLYFGSVFWMVHLEAKRAGLKGLPKDECPSAMAAVKERW 341 +M E + +P+ EI K AL+PA+ + ++ +MVHLEA + ++ +P+ P ++ERW Sbjct: 269 LMVEYVGIPYSEIVKHALLPAVFSYIALLYMVHLEAVKLNMQPIPQRPTP-----MRERW 323 Query: 342 Y--------------LLIPLVVLVWLLFSGRTPMFAGTIGLALTAIVILGS--------- 378 LL ++ + F P+ G+AL I S Sbjct: 324 LRMGLGLAGSVLAICLLYYGIIAIRAAFGESAPLLLAIAGVALYIATIWYSSRYPDLELD 383 Query: 379 ---AIILKVS----------NFALRI-----------------AFWIALGLLCAGFFQLG 408 A IL++ +F + I AFW L ++ + Sbjct: 384 DPDAPILELPRAWDVTRTGLDFLIPIVVLLWCLMVEQLSPGLSAFWATLTIIAIVATRKP 443 Query: 409 IGVIF---GVIAALVAVCWFIKGGRDTLVICLHALVEGARHAVPVGIACALVGVIIGVVS 465 + IF V ++ A W + G L GAR+ + + +A A G+++G ++ Sbjct: 444 LMAIFRKEDVAGSVRAAAWDLTDG----------LALGARNMIGIAVATATAGIVVGTIT 493 Query: 466 LTGVASTFAGYILAVGENNLFLSLLLTMLTCLVLGMGIPTIPNYIITSSIAAPALLDLG- 524 LTG+ + + N+ + L+L LVLGMGIPT NYI+ +++ AP +++LG Sbjct: 494 LTGLGLMMTELVEFISGGNVIMMLILIAAISLVLGMGIPTTANYILVATLMAPVVVELGS 553 Query: 525 -----VPLIVSHMFVFYFGIMADLTPPVALACFAAAPIAKERGLKISMWAIRIAIAGFIV 579 +PLI H+FVFYFGIMAD+TPPV LA FAAA I+KE + ++ I+ Sbjct: 554 QAGLAIPLIAVHLFVFYFGIMADITPPVGLAAFAAAAISKEDPIATGFQGALYSLRTAIL 613 Query: 580 PFMAVYNPALMMQGGD-WGATLYMLFKAAFAVGLWGAVFTGYLQRPMALWEKVL 632 PF+ ++NPA+++ G D W T+++ + A+ ++ A + LWE + Sbjct: 614 PFVFIFNPAILLIGVDTWAHTIWVAAISLAAILIFSAATMNWFVTKSRLWESAV 667 Lambda K H 0.330 0.143 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1593 Number of extensions: 89 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 4 Number of HSP's successfully gapped: 2 Length of query: 674 Length of database: 827 Length adjustment: 40 Effective length of query: 634 Effective length of database: 787 Effective search space: 498958 Effective search space used: 498958 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory