Alignments for a candidate for nagF in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Sinorhizobium meliloti 1021

Align N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA (characterized)
to candidate SMc02437 SMc02437 phosphoenolpyruvate phosphotransferase PTSP (enzyme I-Ntr) protein

Query= TCDB::Q9HXN5
         (842 letters)



>FitnessBrowser__Smeli:SMc02437
          Length = 755

 Score =  264 bits (674), Expect = 2e-74
 Identities = 201/600 (33%), Positives = 290/600 (48%), Gaps = 25/600 (4%)

Query: 240 EDSEAALGALLAALASATAGAPKDAPRAIAPG---EPARPAAVAGTLAGVCASPGLASGP 296
           ED   AL      LA   A    +  +   PG   + +RP  + G   G     G     
Sbjct: 143 EDEVEALETTAMVLAEMVATG--ELKKITKPGLELDLSRPVTIEGNSYGEGIGLGYVVLH 200

Query: 297 LARLGAISLPADDGRHRPEEQHLALDQALQRVRDDVQGSLQQARLGGDENEAAIFSAHLA 356
             R+   +L  +D  H  E Q LA  +AL  +R  +   L +  +  +    A+   +  
Sbjct: 201 EPRIVVTNLLNEDTEH--ELQRLA--EALGSLRISIDDMLSRRDVSMEGEHRAVLETYRM 256

Query: 357 LLEDPGLLDAADMLIDQGVGAAHAWHRAIQAQCEILQALGNLLLAERANDLRDLEKRVLR 416
              D G +   +  I  G+ A  A  R        +  L +  L ER +D  DL  R+LR
Sbjct: 257 FAHDRGWVRKLEEAIRNGLTAEAAVERVQSETKARMIRLTDPYLRERMHDFDDLANRLLR 316

Query: 417 VLLGDTAPLRV---PAGAIVAAREITPSDLAPLVDAGAAGLCMAEGGATSHVAILARSKG 473
            L G  A L     P  AIV AR +  ++L         GL + EG  TSHV I+AR+ G
Sbjct: 317 QLSGYGAKLSAADFPNDAIVVARAMGAAELLDYPRENVRGLVLEEGAVTSHVVIVARAMG 376

Query: 474 LPCLVALGAGLLELEEGRQ-VVLDAGQGRLELSPDARRLEQVALQVAQREEQRRRQQADA 532
           +P +V   AG + L E R  +++D    ++ L P A    Q A +   R   RR+ Q  A
Sbjct: 377 IP-VVGQAAGAVALAENRDAIIVDGDDAKVHLRPMADL--QRAYEEKVRFRARRQAQFRA 433

Query: 533 QR--EALTRDGRRIEIGANVASPREAAEAFANGADGVGLLRTEFLFLERRAAPDEEEQRN 590
            R  E LT+DG+RI +  N     +       GA+G+GL RTE  F+     P  EEQ  
Sbjct: 434 LRDVEPLTKDGKRITLQMNAGLLVDLPHLNEAGAEGIGLFRTELQFMIASTMPKAEEQEA 493

Query: 591 AYQEVLDAMGQRKVIIRTIDVGGDKHLDYLPLPVEENPALGLRGIHLGQARPELLDQQLR 650
            Y+ V+     + V  RT+D+GGDK + Y     EENPALG R I L   RP LL  Q R
Sbjct: 494 FYRNVMKQTAGKPVTFRTLDIGGDKVVPYFRAAEEENPALGWRAIRLSLDRPGLLRTQFR 553

Query: 651 ALLRVEPLERCRILLPMVSEVDELRAIRRRLG---ELATQLGIERLP---ELGVMIEVPS 704
           A+LR       +++LPMV+EV EL++ R  L    E  ++LG E+LP   + G M+EVP+
Sbjct: 554 AMLRATAGAELKMMLPMVTEVAELKSARELLQKEIERQSKLG-EQLPRKLQFGAMLEVPA 612

Query: 705 AALLADQLAEHADFLSIGTNDLSQYALAMDRCHAGLADRIDALHPALLRLIAQTCAGAAR 764
                D+L    DF+S+G+NDL Q+A+A+DR +A +++R D L    LR++ +      R
Sbjct: 613 LLWQLDELMAEVDFVSVGSNDLFQFAMAVDRGNARVSERFDILGRPFLRILREIVRAGER 672

Query: 765 HGRWVGVCGALASDPLATPVLVGLGVEELSVGPNLVGEIKTRVRQLDAAECRRHAQALLD 824
           +   V +CG +AS PL+   L+GLG   +S+ P  VG +K  +  LDA       +A LD
Sbjct: 673 NNTPVTLCGEMASKPLSAMALLGLGFRSVSMSPTAVGPVKAMLLALDAGRLNELLEAALD 732


Lambda     K      H
   0.319    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1258
Number of extensions: 61
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 842
Length of database: 755
Length adjustment: 41
Effective length of query: 801
Effective length of database: 714
Effective search space:   571914
Effective search space used:   571914
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources