Align Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale)
to candidate SM_b20661 SM_b20661 sugar uptake ABC transporter ATP-binding protein
Query= uniprot:D4GP38 (383 letters) >FitnessBrowser__Smeli:SM_b20661 Length = 355 Score = 291 bits (744), Expect = 3e-83 Identities = 170/368 (46%), Positives = 227/368 (61%), Gaps = 22/368 (5%) Query: 1 MGQIQLTDLTKRFGDTVAVDDLSLDIDDEEFLVLVGPSGCGKSTTLRMLAGLETPTSGDI 60 M + D+ K FG + ++++I+D EF++LVGPSGCGKST LRMLAGLE T+G+I Sbjct: 1 MAGVDFVDVRKSFGAFPVIKGVNIEIEDGEFVILVGPSGCGKSTLLRMLAGLENITAGEI 60 Query: 61 YIGGDHMNYRVPQNRDIAMVFQDYALYPHMTVRQNIRFGLEEEEGYTSAERDERVVEVAE 120 IG +N P++RDIAMVFQ+YALYPHMTV N+ F L +E D+RV AE Sbjct: 61 RIGNQVVNRLPPKDRDIAMVFQNYALYPHMTVADNMAFSLMLA-ARPKSEIDKRVGVAAE 119 Query: 121 TLGIADLLDRKPDELSGGQQQRVALGRAIVRDPEVFLMDEPLSNLDAKLRAEMRTELQNL 180 LG++ LLDR P +LSGGQ+QRVA+GRAIVRDP+VFL DEPLSNLDAKLR MR E++ L Sbjct: 120 ILGLSKLLDRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEPLSNLDAKLRVAMRAEIKEL 179 Query: 181 QDQLAVTTVYVTHNQTEAMTMADRIAVMDDGELQQVASPFECYHEPNNLFVAEFIGEPMI 240 +L TTVYVTH+Q EAMTMAD+I VM DG ++Q+ +P E Y P NLFVA FIG P + Sbjct: 180 HQRLKTTTVYVTHDQIEAMTMADKIVVMHDGIVEQIGAPLELYDNPANLFVAGFIGSPAM 239 Query: 241 NLVRG---TRSESTFV-GEHFSYPLDEDVMESVDDRDDFVLGVRPEDIEVADAAPDDAAL 296 N+++G S F+ + + P+ + D V G+RPE + AL Sbjct: 240 NMLKGRLDPADPSVFLTADGTALPVARPA--AAAQGRDLVYGLRPEYM----------AL 287 Query: 297 DDHDLQMDVTVVEPHGDQNVLHLSHPDQPSADDALQAVTEGMHLVTRGDRVTVTIPPDKI 356 D + L ++ V+EP G + L + D E ++ G+ + + I + Sbjct: 288 DPNGLPAEIAVIEPTGYETQLIA----RLGGHDVTCVFRERVN-AKPGETIHLAIDAAHV 342 Query: 357 HLFDAETG 364 HLFDA TG Sbjct: 343 HLFDAGTG 350 Lambda K H 0.317 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 340 Number of extensions: 7 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 355 Length adjustment: 30 Effective length of query: 353 Effective length of database: 325 Effective search space: 114725 Effective search space used: 114725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory