Aligments for a candidate for astD in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Aligments for a candidate for astD in Sinorhizobium meliloti 1021

Align N-succinylglutamate 5-semialdehyde dehydrogenase 1; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase 1; SGSD 1 (uncharacterized)
to candidate SMa0260 SMa0260 GabD3 succinate-semialdehyde dehdyrogenase

Query= curated2:Q9AAL5
         (472 letters)



>FitnessBrowser__Smeli:SMa0260
          Length = 487

 Score =  190 bits (483), Expect = 8e-53
 Identities = 158/477 (33%), Positives = 219/477 (45%), Gaps = 46/477 (9%)

Query: 2   SASRLISRDPYTGEAIADFAVNDARSIDAACHSARAAFAEWAMTPLAERRAIALRFAETV 61
           S S +   DP TG  +A+ A         A  +A AA A W  TP  +R  I  R+ + +
Sbjct: 28  SGSGITVLDPSTGNLLAEVADASIEDAQRAVDAADAAAAGWRATPARQRSEILRRWYQLM 87

Query: 62  RARREEIATLIARETGKPMWEALTEADSVAAKVAISIRAQDERA----GE-RSEPMADAT 116
               EE+ATLIA E GK    AL +A    A  A   R   E A    GE R  P     
Sbjct: 88  TQHAEELATLIALENGK----ALADARGEVAYAAEFFRWYAEEATRIPGEFRHTPSGSHN 143

Query: 117 ARLAHRPHGVLAVIGPFNFPMHLANGHIVPALLAGNAVVFKPSEKTPACGQLMGELWRAA 176
             + H P G+  +I P+NFP  +A   I PAL AG  V+ KP+ +TP     M  L   A
Sbjct: 144 ILVDHEPIGIAVLITPWNFPAAMATRKIGPALAAGCTVILKPASETPLTAYAMARLGEEA 203

Query: 177 GLPDHVLTIVIGGGEAG--EALVRHEALDGVLFTGGVQAGRAIHRALADAPHKIL--ALE 232
           G+P  V+ ++      G   A++    +  + FTG    GR +   LA+A   ++  ++E
Sbjct: 204 GVPPGVVNVLTTSNPGGITNAMLADPRVRKLSFTGSTGVGRVL---LAEAAKSVVSCSME 260

Query: 233 LGGNAPLVVWDVADIEAAAHLIVQSAYVTAGQRCTCARRLILPEGARGDALLEALTMLMD 292
           LGGNAP +V+D AD+E A    + +    AG+ CT A R  +  G   DA +  LT  M 
Sbjct: 261 LGGNAPFIVFDDADLEVALDGAMIAKMRNAGEACTAANRFYVQAGIH-DAFVAGLTARMK 319

Query: 293 RLVIGGPFQSPAPFMGPVIDAHA-------AAQVLAAQDRMTADGGRPLRLAAVREARSA 345
            L + GP   P    GP+I  +A        ++ LAA  R T  GG+PL           
Sbjct: 320 SLKL-GPGYDPETQCGPMITQNAVRKIDRLVSEALAAGARATT-GGKPL------TENGY 371

Query: 346 LLSPGLIE--LTDAPLRDEEIFGPLLQVRRAADFDAALALANATRFGLAAGLISDDEALY 403
              P ++E    +A +  EEIFGP+  V +    D A+ LAN T +GLAA + S D    
Sbjct: 372 FYPPTVLENVPVNASIAREEIFGPVAPVYKFESDDEAIRLANNTEYGLAAYIYSRDLKRA 431

Query: 404 RRFWTSVRAGIVNWNRPTTGASSAAPFGGVGGSGNHRPSA-----------YYAADY 449
            +    +  G++  NR    +  AAPFGGV  SG  R              Y+A DY
Sbjct: 432 MKVGKRIETGMLGINRGLM-SDPAAPFGGVKQSGLGREGGVTGILEFMEPKYFAVDY 487


Lambda     K      H
   0.320    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 603
Number of extensions: 27
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 472
Length of database: 487
Length adjustment: 34
Effective length of query: 438
Effective length of database: 453
Effective search space:   198414
Effective search space used:   198414
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources