GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bgtA in Sinorhizobium meliloti 1021

Align ATPase (characterized, see rationale)
to candidate SMa0489 SMa0489 ABC transporter ATP-binding protein

Query= uniprot:Q31RN8
         (261 letters)



>FitnessBrowser__Smeli:SMa0489
          Length = 255

 Score =  281 bits (720), Expect = 7e-81
 Identities = 145/243 (59%), Positives = 182/243 (74%), Gaps = 1/243 (0%)

Query: 19  ETMIYAEGVEKWYGNQFQALCGVSLTVQRGEVVVMMGPSGSGKSTFLRTLNALESHQRGE 78
           +TMI   GV K +G QF+AL  VSL V+RGE +V+ GPSGSGKST +R +N +E H  G 
Sbjct: 12  DTMIQLIGVGKRFG-QFEALKQVSLEVRRGEKIVLCGPSGSGKSTLIRCINRMEEHTSGR 70

Query: 79  IWIEGHRLSHDRRDIATIRQEVGMVFQQFNLFPHLTVLQNLMLAPVQVRRWPVAQAEATA 138
           I I+G  L+   +DI  +R+EVGMVFQ FNLFPH+T+L+NL +A   VR+ P  +A+  A
Sbjct: 71  IIIDGRELTDRTKDINAVRREVGMVFQSFNLFPHMTILKNLTIAQRLVRKTPEKEAKEVA 130

Query: 139 RQLLERVRIAEQADKYPGQLSGGQQQRVAIARALAMQPRILLFDEPTSALDPEMVREVLD 198
              L+RV+I EQA KYP QLSGGQQQRVAIARAL M+P+I+LFDEPTSALDPEM+ EVLD
Sbjct: 131 MHYLKRVKIPEQASKYPVQLSGGQQQRVAIARALCMKPQIMLFDEPTSALDPEMISEVLD 190

Query: 199 VMRDLASEGMTMLVATHEVGFAREVADRVVLMADGQIVEEAPPDRFFTAPQSDRAKQFLA 258
           VM DLA +GMTM+  THE+GFAR VADRV+ M  GQ++EE  P+ FF  P+++R   FL 
Sbjct: 191 VMVDLARDGMTMICVTHEMGFARSVADRVMFMDGGQLIEEGDPETFFANPRNERTALFLR 250

Query: 259 QIL 261
           QIL
Sbjct: 251 QIL 253


Lambda     K      H
   0.321    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 232
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 255
Length adjustment: 24
Effective length of query: 237
Effective length of database: 231
Effective search space:    54747
Effective search space used:    54747
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory