Alignments for a candidate for pgmA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for pgmA in Sinorhizobium meliloti 1021

Align phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) (EC 5.4.2.2) (characterized)
to candidate SMc03925 SMc03925 phosphoglucomutase

Query= BRENDA::Q9SCY0
         (623 letters)



>FitnessBrowser__Smeli:SMc03925
          Length = 542

 Score =  607 bits (1566), Expect = e-178
 Identities = 305/555 (54%), Positives = 405/555 (72%), Gaps = 14/555 (2%)

Query: 69  IKSLPTKPIEGQKTGTSGLRKKVKVFMEDNYLANWIQALFNSLPLEDYKNATLVLGGDGR 128
           I+++ T P   QK GTSGLRKKV VF + NY  N+IQ++F+SL  E ++  TLV+GGDGR
Sbjct: 2   IRTVSTNPYGDQKPGTSGLRKKVPVFQQKNYAENFIQSIFDSL--EGFEGQTLVIGGDGR 59

Query: 129 YFNKEASQIIIKIAAGNGVGQILVGKEGILSTPAVSAVIRKRKANGGFIMSASHNPGGPE 188
           Y+N+E  Q  +K+AA NG G++LVG+ GILSTPA S VIRK KA GG ++SASHNPGGP 
Sbjct: 60  YYNREVIQKAVKMAAANGFGRVLVGRGGILSTPAASNVIRKYKAFGGIVLSASHNPGGPT 119

Query: 189 YDWGIKFNYSSGQPAPETITDKIYGNTLSISEIKVAEIPDIDLSQVGVTKYGNFSVEVID 248
            D+GIK+N  +G PAPE +TD IY  + +I   K+A+ PD++L   G  +  + +V VID
Sbjct: 120 EDFGIKYNVGNGGPAPEKVTDAIYSRSKAIDSYKIADAPDVNLDVEGSQQVEDMTVTVID 179

Query: 249 PVSDYLELMEDVFDFDLIRGLLSRSDFGFMFDAMHAVTGAYAKPIFVDNLGAKPDSISNG 308
           PV+DY ELME +FDFD IR L++   F  +FDAM AVTG YAK I    LGA   S+ N 
Sbjct: 180 PVADYAELMESLFDFDAIRKLIA-GGFRVVFDAMSAVTGPYAKEIIEKRLGAPKGSVMNF 238

Query: 309 VPLEDFGHGHPDPNLTYAKDLVDVMYRDNGPDFGAASDGDGDRNMVLGNKFFVTPSDSVA 368
           +PL DFG  HPDPNL +A+ L + M   + PDFGAASDGDGDRN+++G   FVTPSDS+A
Sbjct: 239 IPLPDFGGHHPDPNLVHARALYETMMAPDAPDFGAASDGDGDRNLIIGKGIFVTPSDSLA 298

Query: 369 IIAANAQEAIPYFRAGPKGLARSMPTSGALDRVAEKLKLPFFEVPTGWKFFGNLMDAGKL 428
           ++AANA  A P +  G  G+ARSMPTSGA DRVAEKL +  +E PTGWKFFGNL+D G  
Sbjct: 299 MLAANAHLA-PGYAKGLAGIARSMPTSGAADRVAEKLGVGIYETPTGWKFFGNLLDEGLA 357

Query: 429 SICGEESFGTGSDHIREKDGIWAVLAWLSILAHRNKDTKPGDKLVSVADVVKEYWATYGR 488
           +ICGEES GTGS+H+REKDG+WAVL WL+ILA R +         S  ++ +++WATYGR
Sbjct: 358 TICGEESAGTGSNHVREKDGLWAVLLWLNILAARKE---------SALEIARKHWATYGR 408

Query: 489 NFFSRYDYEECESEGANKMIEYLREILSKSKAGDVYGNYVLQFADDFSYTDPVDGSVASK 548
           N++SR+DYEE +++ AN +I  LR+ L+ +  G  +G   ++ ADDFSY DPVD SV+  
Sbjct: 409 NYYSRHDYEEVDTDAANGLIAALRDKLA-ALPGKSFGALTVETADDFSYHDPVDKSVSKN 467

Query: 549 QGVRFVFTDGSRIIFRLSGTGSAGATVRIYIEQFEPDVSKHDVDAQIALKPLIDLALSVS 608
           QGVR +F  GSR++FRLSGTG++GAT+R+YIE++EPD ++HD+D Q AL  LI +A  ++
Sbjct: 468 QGVRILFKGGSRVVFRLSGTGTSGATLRVYIERYEPDPTRHDLDTQEALADLIAVADEIA 527

Query: 609 KLKDFTGREKPTVIT 623
           ++K  TGR++P+VIT
Sbjct: 528 EIKANTGRDEPSVIT 542


Lambda     K      H
   0.317    0.136    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1019
Number of extensions: 59
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 623
Length of database: 542
Length adjustment: 36
Effective length of query: 587
Effective length of database: 506
Effective search space:   297022
Effective search space used:   297022
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources