Alignments for a candidate for nupA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for nupA in Sinorhizobium meliloti 1021

Align Purine/cytidine ABC transporter ATP-binding protein, component of General nucleoside uptake porter, NupABC/BmpA (transports all common nucleosides as well as 5-fluorocytidine, inosine, deoxyuridine and xanthosine) (Martinussen et al., 2010) (Most similar to 3.A.1.2.12). NupA is 506aas with two ABC (C) domains. NupB has 8 predicted TMSs, NupC has 9 or 10 predicted TMSs in a 4 + 1 (or 2) + 4 arrangement (characterized)
to candidate SMa2365 SMa2365 ABC transporter ATP-binding protein

Query= TCDB::A2RKA7
         (506 letters)



>FitnessBrowser__Smeli:SMa2365
          Length = 508

 Score =  390 bits (1001), Expect = e-113
 Identities = 210/500 (42%), Positives = 316/500 (63%), Gaps = 6/500 (1%)

Query: 3   NETVIQMIDVTKRFGDFVANDKVNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEGE 62
           +E ++ +  V+KRFGD +AND ++L L KGEI ALLGENGAGK+TLM+IL G   P  G+
Sbjct: 2   SEAILNICSVSKRFGDNLANDDISLSLGKGEIVALLGENGAGKTTLMSILFGHYVPDSGK 61

Query: 63  VHVKGKLENIDSPSKAANLGIGMVHQHFMLVDAFTVTENIILGNEVTKGINLDLKTAKKK 122
           V V+G+      P  A   GIGMVHQHF L    TV EN++ G E    +      A++K
Sbjct: 62  VLVEGRELPPGKPRAAIRAGIGMVHQHFSLAPNLTVLENVMAGTERLWHLRSGTSAARRK 121

Query: 123 ILELSERYGLSVEPDALIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITELM 182
           +  + +R+GL+VEPDA + D+SVG+QQRVEILK LY  A IL+ DEPTAVLT  E   L 
Sbjct: 122 LHRICQRFGLTVEPDARVGDLSVGEQQRVEILKALYNDAHILVLDEPTAVLTNLEAERLF 181

Query: 183 QIMKNLIKEGKSIILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQ-ELAELMVGR 241
             +K++ +EG S+I I+HKLDE+ A A+RI V+R G+ +    L  +TN+ ELAELMVGR
Sbjct: 182 STLKDMAREGLSLIFISHKLDEVMAAANRIVVLRGGRKV-AERLAKETNKAELAELMVGR 240

Query: 242 SVSFITEKAAAQPKDVVLEIKDLNIKESRGSLKVKGLSLDVRAGEIVGVAGIDGNGQTEL 301
            V+    + +  P +VVL++ D+++    G  ++K +   +RAGE++G+ G+ GNGQT L
Sbjct: 241 RVARPVREPST-PGEVVLKVADVSVSID-GVERLKSIDFSLRAGEVLGIIGVSGNGQTTL 298

Query: 302 VKAITGLTKVDSGSIKLHNKDITNQRPRKITEQSVGHVPEDRHRDGLVLEMTVAENIALQ 361
              ++G  + D G + L  + I +          +G +PEDR+++G + EM + EN  L+
Sbjct: 299 AHLLSGTLRRDKGDLLLFGEPIGDLTVDDAVRAGIGRIPEDRNKEGAIGEMAIWENAVLE 358

Query: 362 TYYKPPMSKYGFLDYNKINSHARELMEEFDVRGAGEWVSASSLSGGNQQKAIIAREIDRN 421
               P  S+YG +D     + A ++++ FDVRG         LSGGN QK I+ R +   
Sbjct: 359 RL--PRFSRYGLVDRPSGQAFAGQIIDAFDVRGGRPTTRTRLLSGGNMQKLILGRNLMDR 416

Query: 422 PDLLIVSQPTRGLDVGAIEYIHKRLIQARDEGKAVLVISFELDEILNVSDRIAVIHDGQI 481
           P +L+ +QP RGLD GA+  +H+RL++AR  G AVL+IS +L+E++ ++DRI  I +G++
Sbjct: 417 PRILLAAQPARGLDEGAVAAVHERLLEARRAGTAVLLISEDLEEVMALADRIQAIVNGRL 476

Query: 482 QGIVSPETTTKQELGILMVG 501
              ++ ++ +  +LG++M G
Sbjct: 477 SPPIAADSASATKLGLMMAG 496



 Score = 73.2 bits (178), Expect = 2e-17
 Identities = 61/229 (26%), Positives = 103/229 (44%), Gaps = 12/229 (5%)

Query: 278 LSLDVRAGEIVGVAGIDGNGQTELVKAITGLTKVDSGSIKLHNKDITNQRPRKITEQSVG 337
           +SL +  GEIV + G +G G+T L+  + G    DSG + +  +++   +PR      +G
Sbjct: 24  ISLSLGKGEIVALLGENGAGKTTLMSILFGHYVPDSGKVLVEGRELPPGKPRAAIRAGIG 83

Query: 338 HVPEDRHRDGLVLEMTVAENIALQTYYKPPMSKYGFLDYNKINSHARE--LMEEFDVRGA 395
            V +      L   +TV EN+   T     +         K++   +   L  E D R  
Sbjct: 84  MVHQ---HFSLAPNLTVLENVMAGTERLWHLRSGTSAARRKLHRICQRFGLTVEPDAR-- 138

Query: 396 GEWVSASSLSGGNQQKAIIAREIDRNPDLLIVSQPTRGLDVGAIEYIHKRLIQARDEGKA 455
                   LS G QQ+  I + +  +  +L++ +PT  L     E +   L     EG +
Sbjct: 139 -----VGDLSVGEQQRVEILKALYNDAHILVLDEPTAVLTNLEAERLFSTLKDMAREGLS 193

Query: 456 VLVISFELDEILNVSDRIAVIHDGQIQGIVSPETTTKQELGILMVGGNI 504
           ++ IS +LDE++  ++RI V+  G+       + T K EL  LMVG  +
Sbjct: 194 LIFISHKLDEVMAAANRIVVLRGGRKVAERLAKETNKAELAELMVGRRV 242


Lambda     K      H
   0.315    0.135    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 663
Number of extensions: 41
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 508
Length adjustment: 34
Effective length of query: 472
Effective length of database: 474
Effective search space:   223728
Effective search space used:   223728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources