Align Purine/cytidine ABC transporter ATP-binding protein, component of General nucleoside uptake porter, NupABC/BmpA (transports all common nucleosides as well as 5-fluorocytidine, inosine, deoxyuridine and xanthosine) (Martinussen et al., 2010) (Most similar to 3.A.1.2.12). NupA is 506aas with two ABC (C) domains. NupB has 8 predicted TMSs, NupC has 9 or 10 predicted TMSs in a 4 + 1 (or 2) + 4 arrangement (characterized)
to candidate SMc04127 SMc04127 ABC transporter ATP-binding protein
Query= TCDB::A2RKA7 (506 letters) >FitnessBrowser__Smeli:SMc04127 Length = 511 Score = 471 bits (1212), Expect = e-137 Identities = 249/502 (49%), Positives = 346/502 (68%), Gaps = 4/502 (0%) Query: 3 NETVIQMIDVTKRFGDFVANDKVNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEGE 62 N I++ + K FG AN +NL ++KG IH ++GENGAGKSTLM+IL G + GE Sbjct: 2 NNIAIELRGIDKSFGLVHANRNINLRVRKGTIHGIIGENGAGKSTLMSILYGFYQADNGE 61 Query: 63 VHVKGKLENIDSPSKAANLGIGMVHQHFMLVDAFTVTENIILGNEVTKGINLDLKTAKKK 122 + V GK +I P+ A ++GIGMVHQHFMLV+ FTV EN++LG E ++ +N + A+++ Sbjct: 62 ILVDGKPVSIRDPNAAISVGIGMVHQHFMLVENFTVLENVMLGAEDSQILNKGIAKARQE 121 Query: 123 ILELSERYGLSVEPDALIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITELM 182 + L + Y L V PDALI ++ VG QQRVEILK LYR ADILI DEPT VLTPAE L Sbjct: 122 LKRLEKEYALEVNPDALIEELPVGLQQRVEILKALYRRADILILDEPTGVLTPAEADHLF 181 Query: 183 QIMKNLIKEGKSIILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQELAELMVGRS 242 +I+ L +GK+IILITHKL EI A+ D ++V+RRG+ + T + + +ELAELMVGR Sbjct: 182 RILGQLKAQGKTIILITHKLREIMAITDEVSVMRRGEMVATRTTRETSVEELAELMVGRR 241 Query: 243 VSFITEKAAAQPKDVVLEIKDLNIKESRGSLKVKGLSLDVRAGEIVGVAGIDGNGQTELV 302 V EK + P DV L ++ L +K+SRG V +S DVRAGEIVG+AG+ GNGQ+EL+ Sbjct: 242 VLLRVEKGESNPGDVKLAVQGLTVKDSRGVTMVDNVSFDVRAGEIVGIAGVAGNGQSELL 301 Query: 303 KAITGLTKVDSGSIKLHNK--DIT-NQRPRKITEQSVGHVPEDRHRDGLVLEMTVAENIA 359 +AI G+ K SG++ L+ + D+T N ++ + + HVPEDRH GLVL+ EN Sbjct: 302 EAIAGIRKAASGTVLLNGRPVDVTGNADAAELRGRGLAHVPEDRHHVGLVLKFEECENAI 361 Query: 360 LQTYYKPPMSKYGFLDYNKINSHARELMEEFDVRGAGEWVSASSLSGGNQQKAIIAREID 419 L ++ P + FL+ + I A E + ++D+R + ++ SGGNQQK ++ARE++ Sbjct: 362 LGYHHDPRFANGMFLNIDAIRKDAEEKIAKYDIRPPNARLRTANFSGGNQQKVVLAREME 421 Query: 420 RNPDLLIVSQPTRGLDVGAIEYIHKRLIQARDEGKAVLVISFELDEILNVSDRIAVIHDG 479 R+PD+LI+ QPTRG+DVGAIE+IHKR+I+ RD+GKAVL++S ELDEI ++SDRI V+ G Sbjct: 422 RDPDVLIIGQPTRGVDVGAIEFIHKRIIEMRDQGKAVLLVSVELDEIRSLSDRILVMFAG 481 Query: 480 QIQGIVSPETTTKQELGILMVG 501 ++ G SPE T+ ELG+LM G Sbjct: 482 RVVGERSPE-ATEGELGLLMAG 502 Score = 84.7 bits (208), Expect = 7e-21 Identities = 59/230 (25%), Positives = 117/230 (50%), Gaps = 10/230 (4%) Query: 276 KGLSLDVRAGEIVGVAGIDGNGQTELVKAITGLTKVDSGSIKLHNKDITNQRPRKITEQS 335 + ++L VR G I G+ G +G G++ L+ + G + D+G I + K ++ + P Sbjct: 22 RNINLRVRKGTIHGIIGENGAGKSTLMSILYGFYQADNGEILVDGKPVSIRDPNAAISVG 81 Query: 336 VGHVPEDRHRDGLVLE-MTVAENIALQTYYKPPMSKYGFLDYNKINSHARELMEEFDVRG 394 +G V H+ +++E TV EN+ L ++K K + L +E+ + Sbjct: 82 IGMV----HQHFMLVENFTVLENVMLGAEDSQILNK----GIAKARQELKRLEKEYALEV 133 Query: 395 AGEWVSASSLSGGNQQKAIIAREIDRNPDLLIVSQPTRGLDVGAIEYIHKRLIQARDEGK 454 + + L G QQ+ I + + R D+LI+ +PT L +++ + L Q + +GK Sbjct: 134 NPDAL-IEELPVGLQQRVEILKALYRRADILILDEPTGVLTPAEADHLFRILGQLKAQGK 192 Query: 455 AVLVISFELDEILNVSDRIAVIHDGQIQGIVSPETTTKQELGILMVGGNI 504 +++I+ +L EI+ ++D ++V+ G++ + T+ +EL LMVG + Sbjct: 193 TIILITHKLREIMAITDEVSVMRRGEMVATRTTRETSVEELAELMVGRRV 242 Lambda K H 0.315 0.135 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 715 Number of extensions: 34 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 506 Length of database: 511 Length adjustment: 34 Effective length of query: 472 Effective length of database: 477 Effective search space: 225144 Effective search space used: 225144 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory