GapMind for catabolism of small carbon sources

 

Aligments for a candidate for etoh-dh-nad in Sinorhizobium meliloti 1021

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate SMc03116 SMc03116 alcohol dehydrogenase

Query= BRENDA::Q76HN6
         (526 letters)



>lcl|FitnessBrowser__Smeli:SMc03116 SMc03116 alcohol dehydrogenase
          Length = 531

 Score =  439 bits (1129), Expect = e-127
 Identities = 246/526 (46%), Positives = 311/526 (59%), Gaps = 8/526 (1%)

Query: 3   FDYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHVNWAF 62
           FDY+IVGAGSAGCVLANRLS DP   V LLEAG  D    IH P+G    +     +W F
Sbjct: 4   FDYIIVGAGSAGCVLANRLSEDPDRRVLLLEAGGSDNYHWIHIPVGYLYCINNPRTDWCF 63

Query: 63  KTTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDVLPYF 122
            T  + GL GR    PRGKVLGG SSINGMIY+RG   D++ W+ LG  GW ++DVLP+F
Sbjct: 64  TTAAEEGLNGRSLGYPRGKVLGGCSSINGMIYMRGQARDYDLWRQLGCPGWSWNDVLPFF 123

Query: 123 RKSEMHHGGSSEYHGGDGELYVSPAN-RHAASEAFVESALRAGHSYNPDFNGATQEGAGY 181
           RKSE H+ G+ + HG  GE  V  A  R A  +AF ++A  AG     DFN    EG+GY
Sbjct: 124 RKSENHYRGADDMHGASGEWRVEKARVRWAVLDAFQKAATEAGIPETDDFNRGNNEGSGY 183

Query: 182 YDVTIRDGRRWSTATAFLKPVRHRSNLTVLTHTHVESIVLLGKQATGVQALIKGSRVHLR 241
           +DV  R G RW+TA AFLKP R+R NLT+LT  HV  ++L   +  GV+    G+    R
Sbjct: 184 FDVNQRSGIRWNTAKAFLKPARNRRNLTILTKAHVRRLILEDGRVAGVEFQHDGTVKSAR 243

Query: 242 ARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDHADVVLCYKSN 301
           AR+E +LSAGA GSPH+L LSGIG    L   GI  RHELP VG+NLQDH  + L YK  
Sbjct: 244 ARRETVLSAGAIGSPHILELSGIGRPDVLHENGIEVRHELPSVGENLQDHLQLRLAYKVT 303

Query: 302 DTSLL---GFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDPGLERPDIQLHSV 358
               L     SL G   +G    +Y   R+GP+A   ++ G F ++ P  E PD+Q H  
Sbjct: 304 GVPTLNEKATSLFGKAAIG---LEYLVRRSGPMAMAPSQLGIFTRSGPEKETPDLQYHVQ 360

Query: 359 IGTVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHDDDVATLL 418
             T++     +H     +  VC LRP+S GSV L  PD   AP I P +L  + D    +
Sbjct: 361 PVTLEKFGEPVHPFPAVTASVCNLRPESRGSVHLKGPDFAAAPDIRPRYLTAEADRDVAV 420

Query: 419 KGYRITRDIIAQTPMASF-GLRDMYSAGLHNDEQLIELLRKRTDTIYHPIGTCKMGQDEM 477
           K  R+TR I+AQ   A +  +          DE+L         TI+HP+GTC+MG D  
Sbjct: 421 KSIRLTRRIVAQPAFARYKPVEFKPGPDYQTDEELKRAAGDIGTTIFHPVGTCRMGSDPD 480

Query: 478 AVVDSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWI 523
           +VVD +LR+ GI GLR+ DASIMPT+  GNTN+  IMIAE+AAE I
Sbjct: 481 SVVDPELRLRGIAGLRIADASIMPTITSGNTNSPTIMIAEKAAEMI 526


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 800
Number of extensions: 31
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 531
Length adjustment: 35
Effective length of query: 491
Effective length of database: 496
Effective search space:   243536
Effective search space used:   243536
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory