Align ABC transporter for L-Fucose, periplasmic substrate-binding protein (characterized)
to candidate SM_b21103 SM_b21103 sugar ABC transporter substrate-binding protein
Query= reanno::Smeli:SM_b21103 (439 letters) >FitnessBrowser__Smeli:SM_b21103 Length = 439 Score = 890 bits (2301), Expect = 0.0 Identities = 439/439 (100%), Positives = 439/439 (100%) Query: 1 MNRLLSGVSAGVIMLACAMGAAKAADLPGKFEGVTIDAKLIGGQQYEKLYERIGEWEKAT 60 MNRLLSGVSAGVIMLACAMGAAKAADLPGKFEGVTIDAKLIGGQQYEKLYERIGEWEKAT Sbjct: 1 MNRLLSGVSAGVIMLACAMGAAKAADLPGKFEGVTIDAKLIGGQQYEKLYERIGEWEKAT 60 Query: 61 GAKVNILSKKNHFELDKEIKSDIATGGLTWCVGSNHSSFAPQYPDIYADLFGLIPSEEVA 120 GAKVNILSKKNHFELDKEIKSDIATGGLTWCVGSNHSSFAPQYPDIYADLFGLIPSEEVA Sbjct: 61 GAKVNILSKKNHFELDKEIKSDIATGGLTWCVGSNHSSFAPQYPDIYADLFGLIPSEEVA 120 Query: 121 KFVPAVIDASTLEGKLVMLPRAQFDVSALYFQKSLYQDEAKKTEFKAKYGYDLAPPDTWA 180 KFVPAVIDASTLEGKLVMLPRAQFDVSALYFQKSLYQDEAKKTEFKAKYGYDLAPPDTWA Sbjct: 121 KFVPAVIDASTLEGKLVMLPRAQFDVSALYFQKSLYQDEAKKTEFKAKYGYDLAPPDTWA 180 Query: 181 QVSDQAEFFAAPPNFYGTQFAGKEEAINGRFYEMLVAEGGEYLDKDGRPAFNSDAGVRAL 240 QVSDQAEFFAAPPNFYGTQFAGKEEAINGRFYEMLVAEGGEYLDKDGRPAFNSDAGVRAL Sbjct: 181 QVSDQAEFFAAPPNFYGTQFAGKEEAINGRFYEMLVAEGGEYLDKDGRPAFNSDAGVRAL 240 Query: 241 EWFVKLYKDKAVPPGTTNYLWDDLGQGFASGSIAVNLDWPGWASFFNDPKSSKVAGNVGV 300 EWFVKLYKDKAVPPGTTNYLWDDLGQGFASGSIAVNLDWPGWASFFNDPKSSKVAGNVGV Sbjct: 241 EWFVKLYKDKAVPPGTTNYLWDDLGQGFASGSIAVNLDWPGWASFFNDPKSSKVAGNVGV 300 Query: 301 KVQPAGSSGKRTGWSGHHGFSVTESCADKEAAASLVWWLTNEDSQKLESAAGPLPTRSAV 360 KVQPAGSSGKRTGWSGHHGFSVTESCADKEAAASLVWWLTNEDSQKLESAAGPLPTRSAV Sbjct: 301 KVQPAGSSGKRTGWSGHHGFSVTESCADKEAAASLVWWLTNEDSQKLESAAGPLPTRSAV 360 Query: 361 WDFNIKAAEGDAYKTEVLQAFQEAAKHAFPVPQTAEWIEISNAVYPELQAAILGDKTSKE 420 WDFNIKAAEGDAYKTEVLQAFQEAAKHAFPVPQTAEWIEISNAVYPELQAAILGDKTSKE Sbjct: 361 WDFNIKAAEGDAYKTEVLQAFQEAAKHAFPVPQTAEWIEISNAVYPELQAAILGDKTSKE 420 Query: 421 ALDAAAEKATGILEDAGKL 439 ALDAAAEKATGILEDAGKL Sbjct: 421 ALDAAAEKATGILEDAGKL 439 Lambda K H 0.314 0.132 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 843 Number of extensions: 21 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 439 Length of database: 439 Length adjustment: 32 Effective length of query: 407 Effective length of database: 407 Effective search space: 165649 Effective search space used: 165649 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory