Aligments for a candidate for gntB in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Aligments for a candidate for gntB in Sinorhizobium meliloti 1021

Align TRAP-type large permease component (characterized, see rationale)
to candidate SM_b20035 SM_b20035 ABC transporter permease

Query= uniprot:Q930R2
         (425 letters)



>lcl|FitnessBrowser__Smeli:SM_b20035 SM_b20035 ABC transporter
           permease
          Length = 426

 Score =  509 bits (1310), Expect = e-149
 Identities = 247/423 (58%), Positives = 331/423 (78%)

Query: 1   MTLVVFIVSLLGAMAIGVPVAFSLMFCGVVLMWYMGMFNTQIIAQNMIAGADTFTLLAIP 60
           MT+ +F+ +LLG MA+GVP+AF+L+  GV LM Y+G+F+ QI+AQN++ GAD+F L+A+P
Sbjct: 1   MTISIFLGALLGPMALGVPIAFALILSGVALMLYLGLFDAQIVAQNVLNGADSFPLMAVP 60

Query: 61  FFILAGELMNAGGLSRRIIDFAIACVGHIRGGLGIVAIMAAVIMASISGSAAADTAALAA 120
           FF+LAGE+MN GGLSRRI+  A+A VGHIRGGLG VAI AA I++S+SGSA AD AAL A
Sbjct: 61  FFLLAGEVMNTGGLSRRIVALAMAMVGHIRGGLGFVAIFAACILSSLSGSAVADAAALGA 120

Query: 121 ILIPMMAKAGYNVPRSAGLIAAGGVIAPVIPPSMAFIVFGVAANVSITQLFMAGIVPGLI 180
           +L+PMM K+G++  R++GLIA+  +I P+IPPS+ FI+FGV   VSIT+LF+AGI PGL+
Sbjct: 121 LLLPMMLKSGHDPARASGLIASASIIGPIIPPSIGFILFGVVGGVSITKLFLAGIFPGLM 180

Query: 181 MGIALVATWLLVVRKDDIQPLPRTPMKERVGATGRALWALGMPVIILGGIKAGVVTPTEA 240
           +  AL  TWL+V RK+  +  PR   + R+ A   +LWAL +PVII+ G+K GV TPTEA
Sbjct: 181 IAAALSITWLIVARKEQFELPPRQSGRLRLRAFVDSLWALFLPVIIIAGLKFGVFTPTEA 240

Query: 241 AVVAAVYALFVGMVIYRELKPRDLPGVILQAAKTTAVIMFLVCAALVSSWLITAANIPSE 300
            V+AAVY+LFV MV+YREL P  L  V + AAK +AV+MFLV  A VS+WLIT A++P  
Sbjct: 241 GVIAAVYSLFVSMVVYRELAPAQLFHVFVSAAKISAVVMFLVACAAVSAWLITVADVPGA 300

Query: 301 ITGFISPLIDRPTLLMFVIMLVVLVVGTALDLTPTILILTPVLMPIIKQAGIDPVYFGVL 360
           +   + PL+   T L+  IM+++++VGTA+D+TPTILI+TPVLMP+IKQAGIDPVYFGVL
Sbjct: 301 LAALLEPLMGNQTALLIAIMVLIVIVGTAMDMTPTILIMTPVLMPVIKQAGIDPVYFGVL 360

Query: 361 FIMNTCIGLLTPPVGVVLNVVSGVGRVPLGKVIVGVTPFLVAQILVLFLLVLFPDIVIVP 420
           FI+N  IGL+TPPVG VLNV+ GV ++ +  ++ GV PFL A+++VLFLLVLFP++V VP
Sbjct: 361 FIINNSIGLITPPVGTVLNVICGVSKLSMEDLMKGVMPFLFAELIVLFLLVLFPELVTVP 420

Query: 421 ARW 423
             W
Sbjct: 421 VSW 423


Lambda     K      H
   0.331    0.145    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 550
Number of extensions: 23
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 425
Length of database: 426
Length adjustment: 32
Effective length of query: 393
Effective length of database: 394
Effective search space:   154842
Effective search space used:   154842
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources