Align ABC transporter for D-Glucosamine, permease component 1 (characterized)
to candidate SM_b21136 SM_b21136 amino acid ABC transporter permease
Query= reanno::pseudo5_N2C3_1:AO356_00470 (220 letters) >FitnessBrowser__Smeli:SM_b21136 Length = 228 Score = 129 bits (323), Expect = 6e-35 Identities = 72/210 (34%), Positives = 117/210 (55%) Query: 11 LWVARDTLWAGFLTSVQCSLLAIVLGTLIGLVAGLVLTYGRTWMRAPFRFYVDLIRGTPV 70 +W + D L G S+ + +AI+ G +IGL+ L R +R P YV +IR TP+ Sbjct: 18 VWRSFDLLLEGLALSLGLAFVAILAGCVIGLITAFGLISRRALLRKPAGLYVTVIRNTPI 77 Query: 71 FVLVLACFYMAPALGWQIGAFQAGVLGLTLFCGSHVAEIVRGALQALPRGQMEASQAIGL 130 VLVL ++ P LG ++G ++ V L ++ G+++AE+ RG L A+P GQ EA AIGL Sbjct: 78 LVLVLFSYFALPELGVRLGKIESFVATLAIYSGAYLAEVFRGGLIAIPPGQREAGLAIGL 137 Query: 131 TFYQSLGYVLLPQALRQILPTWVNSSTEIVKASTLLSVIGVAELLLSTQQIIARTFMTLE 190 T Q +++P LR +LP+ ++ + K ++L + I V EL ++I TF +E Sbjct: 138 TEMQIRTSIIIPLMLRSVLPSLGSTMISLFKDTSLAAAIAVPELTFEARKINVETFRVIE 197 Query: 191 FYLFAGFLFFIINYAIELLGRHIEKRVALP 220 ++ A L+ + L R E+R+A+P Sbjct: 198 TWIVASCLYVATCSLLAALMRAAERRLAVP 227 Lambda K H 0.330 0.142 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 134 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 220 Length of database: 228 Length adjustment: 22 Effective length of query: 198 Effective length of database: 206 Effective search space: 40788 Effective search space used: 40788 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory