GapMind for catabolism of small carbon sources

 

Alignments for a candidate for gtsD in Sinorhizobium meliloti 1021

Align GtsD (GLcK), component of Glucose porter, GtsABCD (characterized)
to candidate SM_b21216 SM_b21216 sugar ABC transporter ATP-binding protein

Query= TCDB::Q88P35
         (384 letters)



>FitnessBrowser__Smeli:SM_b21216
          Length = 360

 Score =  357 bits (915), Expect = e-103
 Identities = 187/367 (50%), Positives = 250/367 (68%), Gaps = 8/367 (2%)

Query: 1   MATLELRNVNKTYGSGLPDTLKDIQLSIKDGEFLILVGPSGCGKSTLMNCIAGLEQITGG 60
           M+ LE+RN+ K YG    +TLK I ++++ GEFL+L+G SGCGKSTL+N IAGL + +GG
Sbjct: 1   MSALEIRNIRKRYGE--VETLKGIDIALESGEFLVLLGSSGCGKSTLLNIIAGLAEPSGG 58

Query: 61  AILIDEQDVSGMSPKDRDIAMVFQSYALYPTMSVRENIEFGLKIRKLPQAAIDEEVARVA 120
            ILI E+ V G+ PKDRDIAMVFQSYALYP +SV  NI FGL++R++PQA  D+ V   A
Sbjct: 59  DILIGERSVLGVHPKDRDIAMVFQSYALYPNLSVARNIGFGLEMRRVPQAEHDKAVRDTA 118

Query: 121 KLLQIEHLLARKPAQLSGGQQQRVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTEMKL 180
           +LLQIE+LL RKP+QLSGGQ+QRVA+GRAL R P+++LFDEPLSNLDAKLR+EMRTE+K 
Sbjct: 119 RLLQIENLLDRKPSQLSGGQRQRVAIGRALVRNPQVFLFDEPLSNLDAKLRMEMRTELKR 178

Query: 181 MHQRLKTTTVYVTHDQIEAMTLGDKVAVMKDGIIQQFGTPQQIYNDPANQFVASFIGSPP 240
           +HQ L+TT VYVTHDQIEAMTL  ++AVM+DG I+Q   P ++Y+ PA  +VA F+GSPP
Sbjct: 179 LHQMLRTTVVYVTHDQIEAMTLATRIAVMRDGRIEQLAAPDEVYDRPATLYVAGFVGSPP 238

Query: 241 MNFIPVRLARQDGRLLALLDSGQARCELPLGEAADALEGREIILGIRPEQIALGAADGNG 300
           MN +   +     +    ++  +    LP      A  GR + +GIRPE + L A  G+ 
Sbjct: 239 MNILDAEMTANGLK----IEGCEEVLPLPAAFNGAAWAGRRVKVGIRPEALRLAA--GSE 292

Query: 301 LPAIRAEVQVTEPTGPDLLVFVTLNQTKVCCRLAPDVACRVGDTLNLQFDPARVLLFDAA 360
              + A V+V E TGP+L+   T+   ++   L P  A  +G      FD   + LFD  
Sbjct: 293 AQRLTASVEVVELTGPELVTTATVGSQRITACLPPRTAVGMGSAHAFTFDGTALHLFDPE 352

Query: 361 NGERLHL 367
           +G  L +
Sbjct: 353 SGRSLRM 359


Lambda     K      H
   0.320    0.137    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 387
Number of extensions: 10
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 384
Length of database: 360
Length adjustment: 30
Effective length of query: 354
Effective length of database: 330
Effective search space:   116820
Effective search space used:   116820
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory